| RRC ID |
17560
|
| 著者 |
Geffeney SL, Cueva JG, Glauser DA, Doll JC, Lee TH, Montoya M, Karania S, Garakani AM, Pruitt BL, Goodman MB.
|
| タイトル |
DEG/ENaC but not TRP channels are the major mechanoelectrical transduction channels in a C. elegans nociceptor.
|
| ジャーナル |
Neuron
|
| Abstract |
Many nociceptors detect mechanical cues, but the ion channels responsible for mechanotransduction in these sensory neurons remain obscure. Using in vivo recordings and genetic dissection, we identified the DEG/ENaC protein, DEG-1, as the major mechanotransduction channel in ASH, a polymodal nociceptor in Caenorhabditis elegans. But DEG-1 is not the only mechanotransduction channel in ASH: loss of deg-1 revealed a minor current whose properties differ from those expected of DEG/ENaC channels. This current was independent of two TRPV channels expressed in ASH. Although loss of these TRPV channels inhibits behavioral responses to noxious stimuli, we found that both mechanoreceptor currents and potentials were essentially wild-type in TRPV mutants. We propose that ASH nociceptors rely on two genetically distinct mechanotransduction channels and that TRPV channels contribute to encoding and transmitting information. Because mammalian and insect nociceptors also coexpress DEG/ENaCs and TRPVs, the cellular functions elaborated here for these ion channels may be conserved.
|
| 巻・号 |
71(5)
|
| ページ |
845-57
|
| 公開日 |
2011-9-8
|
| DOI |
10.1016/j.neuron.2011.06.038
|
| PII |
S0896-6273(11)00596-4
|
| PMID |
21903078
|
| PMC |
PMC3170654
|
| MeSH |
Amiloride / pharmacology
Animals
Animals, Genetically Modified
Behavior, Animal / physiology
Biophysical Phenomena / drug effects
Biophysical Phenomena / genetics
Biophysical Phenomena / physiology*
Caenorhabditis elegans
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / physiology*
Electric Stimulation / methods
Mechanotransduction, Cellular / genetics
Mechanotransduction, Cellular / physiology*
Membrane Potentials / drug effects
Membrane Potentials / genetics*
Membrane Proteins / genetics
Membrane Proteins / physiology*
Mutation, Missense / genetics
Nociceptors / metabolism*
Patch-Clamp Techniques / methods
Reaction Time / drug effects
Reaction Time / genetics
Sodium / metabolism
Sodium Channel Blockers / pharmacology
TRPC Cation Channels / metabolism*
Touch / physiology
|
| IF |
14.415
|
| 引用数 |
66
|
|
WOS 分野
|
NEUROSCIENCES
|
| リソース情報 |
| 線虫 |
unc-8(tm2701) |
