RRC ID 15394
著者 Lee G, Liang C, Park G, Jang C, Jung JU, Chung J.
タイトル UVRAG is required for organ rotation by regulating Notch endocytosis in Drosophila.
ジャーナル Dev Biol
Abstract Heterotaxy characterized by abnormal left-right body asymmetry causes diverse congenital anomalies. Organ rotation is a crucial developmental process to establish the left-right patterning during animal development. However, the molecular basis of how organ rotation is regulated is poorly understood. Here we report that Drosophila UV-resistance associated gene (UVRAG), a tumor suppressor that regulates autophagy and endocytosis, plays unexpected roles in controlling organ rotation. Loss-of-function mutants of UVRAG show seriously impaired organ rotation phenotypes, which are associated with defects in endocytic trafficking rather than autophagy. Blunted endocytic degradation by UVRAG deficiency causes endosomal accumulation of Notch, resulting in abnormally enhanced Notch activity. Knockdown of Notch itself or expression of a dominant negative form of Notch transcriptional co-activator Mastermind is sufficient to rescue the rotation defect in UVRAG mutants. Consistently, UVRAG-mutated heterotaxy patient cells also display highly increased Notch protein levels. These results suggest evolutionarily conserved roles of UVRAG in organ rotation by regulating Notch endocytic degradation.
巻・号 356(2)
ページ 588-97
公開日 2011-8-15
DOI 10.1016/j.ydbio.2011.06.024
PII S0012-1606(11)01083-9
PMID 21729695
PMC PMC4414087
MeSH Animals Autophagy Cell Proliferation Drosophila Proteins / metabolism* Drosophila melanogaster / embryology* Endocytosis* HCT116 Cells Humans Receptors, Notch / metabolism* Rotation Tumor Suppressor Proteins / metabolism*
IF 2.896
引用数 29
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
ショウジョウバエ 6116R-1 6116R-2 2411R-1 GS17330 (Drosophila Genetic Resource Center Kyoto Institute of Technology Japan)