RRC ID 12784
著者 Shibata Y, Uchida M, Takeshita H, Nishiwaki K, Sawa H.
タイトル Multiple functions of PBRM-1/Polybromo- and LET-526/Osa-containing chromatin remodeling complexes in C. elegans development.
ジャーナル Dev Biol
Abstract The SWI/SNF-like chromatin remodeling complexes consist of two evolutionarily conserved subclasses, which are characterized by specific accessory components, the OSA/BAF250 and Polybromo proteins. These complexes regulate the expressions of distinct sets of target genes, with some overlap, and the regulatory components are thought to determine the target specificity for each complex. Here we isolated C. elegans mutants of the genes for the OSA/BAF250 homolog, LET-526, and the Polybromo homolog, PBRM-1, in a screen for the abnormal asymmetric cell division phenotype. In the asymmetric division of the T cell, both LET-526 and PBRM-1 regulated the asymmetric expression of psa-3/Meis between the T cell daughters, suggesting that the two subclasses share the same target. In the gonad, PBRM-1 regulated gonad primordium formation during embryogenesis, whereas LET-526 was required post-embryonically for distal tip cell (DTC) production from the gonad primordium, suggesting that these proteins have distinct targets for DTC development. Thus, the same cellular process is regulated by LET-526 and PBRM-1 in the asymmetric division of the T cell, but they regulate distinct cellular processes in the gonad morphogenesis. Although disruption of the core component PSA-1 or PSA-4 caused similar defects in the gonad and T cell, it also caused early embryonic arrest, which was not observed in the let-526, pbrm-1, or let-526 pbrm-1 double mutants, suggesting that some targets of SWI/SNF-like complexes do not require LET-526 or PBRM-1 for their transcription. Our results show that the target selection by SWI/SNF-like complexes during C. elegans development is intricately regulated by accessory components.
巻・号 361(2)
ページ 349-57
公開日 2012-1-15
DOI 10.1016/j.ydbio.2011.10.035
PII S0012-1606(11)01373-X
PMID 22119053
MeSH Animals Caenorhabditis elegans / cytology Caenorhabditis elegans / embryology* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Lineage Chromatin Assembly and Disassembly* Chromosomal Proteins, Non-Histone / metabolism Cloning, Molecular Embryonic Development / genetics Genes, Helminth / genetics Gonads / cytology Gonads / growth & development Gonads / metabolism Mitosis / genetics Mutation / genetics Phenotype Protein Transport Transcription Factors / metabolism Wnt Signaling Pathway / genetics
IF 2.896
引用数 14
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
線虫 tm415