RRC ID 12317
著者 Deguchi T, Fujimori KE, Kawasaki T, Xianghai L, Yuba S.
タイトル Expression patterns of the Egr1 and Egr3 genes during medaka embryonic development.
ジャーナル Gene Expr Patterns
Abstract Egr1 and Egr3 are zinc finger-type transcription factors and known as synaptic activity-inducible immediate-early genes. Egr1 also plays important roles in many aspects of vertebrate development. Egr3 is known as the gene that is related to biological rhythm and muscular development, but its behavior in the central nervous system during development is not clear. We cloned the cDNA of the egr1 and egr3 orthologs in medaka, and examined their expression patterns during embryonic development using whole-mount in situ hybridization. Medaka egr3 was the first cloned egr3 gene in fish. The expression of egr1 mRNA was first detected at 1day post-fertilization (dpf). It was expressed in the whole embryonic body. At 3dpf, the egr1 mRNA was strongly expressed in the telencephalon, diencephalon, hypothalamus, optic tectum, dorsal medulla oblongata, retina, heart, pharynx, and pectoral fin. The expression of egr3 mRNA was first detected at 3dpf. It was expressed in the telencephalon, hypothalamus, optic tectum, and pharynx. By sectioning the whole-mount specimens, expression of both the egr1 and egr3 mRNAs were observed in the telencephalon, hypothalamus, and optic tectum. However, the positions at which the genes were expressed were different.
巻・号 9(4)
ページ 209-14
公開日 2009-4-1
DOI 10.1016/j.gep.2008.12.004
PII S1567-133X(08)00136-1
PMID 19154798
MeSH Amino Acid Sequence Animals Early Growth Response Protein 1 / classification Early Growth Response Protein 1 / genetics* Early Growth Response Protein 3 / classification Early Growth Response Protein 3 / genetics* Embryo, Nonmammalian / embryology Embryo, Nonmammalian / metabolism Fish Proteins / genetics Gene Expression Profiling* Gene Expression Regulation, Developmental* Hypothalamus / embryology Hypothalamus / metabolism In Situ Hybridization Molecular Sequence Data Optic Lobe, Nonmammalian / embryology Optic Lobe, Nonmammalian / metabolism Oryzias / embryology Oryzias / genetics* Phylogeny RNA, Messenger / genetics RNA, Messenger / metabolism Sequence Homology, Amino Acid Telencephalon / embryology Telencephalon / metabolism Time Factors
IF 0.897
引用数 4
WOS 分野 GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY
リソース情報
メダカ HdrR-II1 (IB178) STII