RRC ID 11193
著者 Lin H, Wang Y, Wang Y, Tian F, Pu P, Yu Y, Mao H, Yang Y, Wang P, Hu L, Lin Y, Liu Y, Xu Y, Chen CD.
タイトル Coordinated regulation of active and repressive histone methylations by a dual-specificity histone demethylase ceKDM7A from Caenorhabditis elegans.
ジャーナル Cell Res
Abstract H3K9me2 and H3K27me2 are important epigenetic marks associated with transcription repression, while H3K4me3 is associated with transcription activation. It has been shown that active and repressive histone methylations distribute in a mutually exclusive manner, but the underlying mechanism was poorly understood. Here we identified ceKDM7A, a PHD (plant homeodomain)- and JmjC domain-containing protein, as a histone demethylase specific for H3K9me2 and H3K27me2. We further demonstrated that the PHD domain of ceKDM7A bound H3K4me3 and H3K4me3 co-localized with ceKDM7A at the genome-wide level. Disruption of the PHD domain binding to H3K4me3 reduced the demethylase activity in vivo, and loss of ceKDM7A reduced the expression of its associated target genes. These results indicate that ceKDM7A is recruited to the promoter to demethylate H3K9me2 and H3K27me2 and activate gene expression through the binding of the PHD domain to H3K4me3. Thus, our study identifies a dual-specificity histone demethylase and provides novel insights into the regulation of histone methylation.
巻・号 20(8)
ページ 899-907
公開日 2010-8-1
DOI 10.1038/cr.2010.84
PII cr201084
PMID 20567262
MeSH Animals Caenorhabditis elegans / enzymology* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / metabolism* Histones / metabolism Jumonji Domain-Containing Histone Demethylases / chemistry Jumonji Domain-Containing Histone Demethylases / metabolism* Protein Binding Protein Structure, Tertiary Substrate Specificity
IF 20.507
引用数 23
WOS 分野 CELL BIOLOGY
リソース情報
線虫 tm3713