| RRC ID |
11132
|
| 著者 |
Junion G, Bataillé L, Jagla T, Da Ponte JP, Tapin R, Jagla K.
|
| タイトル |
Genome-wide view of cell fate specification: ladybird acts at multiple levels during diversification of muscle and heart precursors.
|
| ジャーナル |
Genes Dev
|
| Abstract |
Correct diversification of cell types during development ensures the formation of functional organs. The evolutionarily conserved homeobox genes from ladybird/Lbx family were found to act as cell identity genes in a number of embryonic tissues. A prior genetic analysis showed that during Drosophila muscle and heart development ladybird is required for the specification of a subset of muscular and cardiac precursors. To learn how ladybird genes exert their cell identity functions we performed muscle and heart-targeted genome-wide transcriptional profiling and a chromatin immunoprecipitation (ChIP)-on-chip search for direct Ladybird targets. Our data reveal that ladybird not only contributes to the combinatorial code of transcription factors specifying the identity of muscle and cardiac precursors, but also regulates a large number of genes involved in setting cell shape, adhesion, and motility. Among direct ladybird targets, we identified bric-a-brac 2 gene as a new component of identity code and inflated encoding alphaPS2-integrin playing a pivotal role in cell-cell interactions. Unexpectedly, ladybird also contributes to the regulation of terminal differentiation genes encoding structural muscle proteins or contributing to muscle contractility. Thus, the identity gene-governed diversification of cell types is a multistep process involving the transcriptional control of genes determining both morphological and functional properties of cells.
|
| 巻・号 |
21(23)
|
| ページ |
3163-80
|
| 公開日 |
2007-12-1
|
| DOI |
10.1101/gad.437307
|
| PII |
21/23/3163
|
| PMID |
18056427
|
| PMC |
PMC2081981
|
| MeSH |
Animals
Animals, Genetically Modified
Cell Adhesion / genetics
Cell Differentiation / genetics
Cell Fusion
Cell Movement / genetics
Chromatin Immunoprecipitation
Drosophila / cytology*
Drosophila / embryology
Drosophila / genetics*
Gene Expression Profiling
Gene Expression Regulation, Developmental
Gene Targeting
Genes, Homeobox*
Genes, Insect*
Genome, Insect
Models, Biological
Myoblasts / cytology*
Myoblasts, Cardiac / cytology*
RNA Interference
|
| IF |
9.527
|
| 引用数 |
32
|
|
WOS 分野
|
GENETICS & HEREDITY
DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ショウジョウバエ |
31916R-1
4696R-4
16858R-1 |
