RRC ID 11116
著者 Savla U, Benes J, Zhang J, Jones RS.
タイトル Recruitment of Drosophila Polycomb-group proteins by Polycomblike, a component of a novel protein complex in larvae.
ジャーナル Development
Abstract Polycomb-group (PcG) proteins are highly conserved epigenetic transcriptional repressors that play central roles in numerous examples of developmental gene regulation. Four PcG repressor complexes have been purified from Drosophila embryos: PRC1, PRC2, Pcl-PRC2 and PhoRC. Previous studies described a hierarchical recruitment pathway of PcG proteins at the bxd Polycomb Response Element (PRE) of the Ultrabithorax (Ubx) gene in larval wing imaginal discs. The DNA-binding proteins Pho and/or Phol are required for target site binding by PRC2, which in turn is required for chromosome binding by PRC1. Here, we identify a novel larval complex that contains the PcG protein Polycomblike (Pcl) that is distinct from PRC1 and PRC2 and which is also dependent on Pho and/or Phol for binding to the bxd PRE in wing imaginal discs. RNAi-mediated depletion of Pcl in larvae disrupts chromosome binding by E(z), a core component of PRC2, but Pcl does not require E(z) for chromosome binding. These results place the Pcl complex (PCLC) downstream of Pho and/or Phol and upstream of PRC2 and PRC1 in the recruitment hierarchy.
巻・号 135(5)
ページ 813-7
公開日 2008-3-1
DOI 10.1242/dev.016006
PII dev.016006
PMID 18216170
MeSH Animals Cell Nucleus / physiology* Chromatin / isolation & purification Chromatography, Gel Chromosomes / physiology Drosophila / genetics Drosophila / growth & development* Drosophila Proteins / genetics Drosophila Proteins / isolation & purification Drosophila Proteins / physiology* Gene Expression Regulation, Developmental* Genome Larva Polycomb Repressive Complex 1 Protein Binding Repressor Proteins / genetics Repressor Proteins / isolation & purification Repressor Proteins / physiology* Wings, Animal / embryology Wings, Animal / growth & development
IF 5.611
引用数 53
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
情報 nigfly
ショウジョウバエ 5109R-1