RRC ID 11085
著者 Wang L, Jahren N, Miller EL, Ketel CS, Mallin DR, Simon JA.
タイトル Comparative analysis of chromatin binding by Sex Comb on Midleg (SCM) and other polycomb group repressors at a Drosophila Hox gene.
ジャーナル Mol Cell Biol
Abstract Sex Comb on Midleg (SCM) is a transcriptional repressor in the Polycomb group (PcG), but its molecular role in PcG silencing is not known. Although SCM can interact with Polycomb repressive complex 1 (PRC1) in vitro, biochemical studies have indicated that SCM is not a core constituent of PRC1 or PRC2. Nevertheless, SCM is just as critical for Drosophila Hox gene silencing as canonical subunits of these well-characterized PcG complexes. To address functional relationships between SCM and other PcG components, we have performed chromatin immunoprecipitation studies using cultured Drosophila Schneider line 2 (S2) cells and larval imaginal discs. We find that SCM associates with a Polycomb response element (PRE) upstream of the Ubx gene which also binds PRC1, PRC2, and the DNA-binding PcG protein Pleiohomeotic (PHO). However, SCM is retained at this Ubx PRE despite genetic disruption or knockdown of PHO, PRC1, or PRC2, suggesting that SCM chromatin targeting does not require prior association of these other PcG components. Chromatin immunoprecipitations (IPs) to test the consequences of SCM genetic disruption or knockdown revealed that PHO association is unaffected, but reduced levels of PRE-bound PRC2 and PRC1 were observed. We discuss these results in light of current models for recruitment of PcG complexes to chromatin targets.
巻・号 30(11)
ページ 2584-93
公開日 2010-6-1
DOI 10.1128/MCB.01451-09
PII MCB.01451-09
PMID 20351181
PMC PMC2876513
MeSH Amino Acid Sequence Animals Chromatin / metabolism* DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / anatomy & histology Drosophila melanogaster / genetics Drosophila melanogaster / metabolism Female Gene Silencing Genes, Homeobox* Homeodomain Proteins / genetics Homeodomain Proteins / metabolism Humans Male Molecular Sequence Data Polycomb-Group Proteins Protein Binding RNA Interference Repressor Proteins / genetics Repressor Proteins / metabolism* Sequence Alignment Transcription Factors / genetics Transcription Factors / metabolism Wings, Animal / anatomy & histology Wings, Animal / physiology
IF 3.611
引用数 35
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
ショウジョウバエ 32443R-4