論文 - 詳細
| RRC ID | 11041 |
|---|---|
| 著者 | Walker J, Kwon SY, Badenhorst P, East P, McNeill H, Svejstrup JQ. |
| タイトル | Role of elongator subunit Elp3 in Drosophila melanogaster larval development and immunity. |
| ジャーナル | Genetics |
| Abstract |
The Elongator complex has been implicated in several cellular processes, including gene expression and tRNA modification. We investigated the biological importance of the Elp3 gene in Drosophila melanogaster. Deletion of Elp3 results in larval lethality at the pupal stage. During early development, larval growth is dramatically impaired, with progression to the third instar delayed for ∼24 hr, and pupariation occurring only at day 14 after egg laying. Melanotic nodules appear after 4 days. Microarray analysis shows that stress response genes are induced and ecdysone-induced transcription factors are severely repressed in the mutant. Interestingly, the phenotypes of Elp3 flies are similar to those of flies lacking the domino gene, encoding a SWI/SNF-like ATP-dependent chromatin-remodeling enzyme. Indeed, the gene expression profiles of these mutants are also remarkably similar. Together, these data demonstrate that Drosophila Elp3 is essential for viability, normal development, and hematopoiesis and suggest a functional overlap with the chromatin remodeler Domino. |
| 巻・号 | 187(4) |
| ページ | 1067-75 |
| 公開日 | 2011-4-1 |
| DOI | 10.1534/genetics.110.123893 |
| PII | genetics.110.123893 |
| PMID | 21288872 |
| PMC | PMC3070516 |
| MeSH | Animals Chromatin / genetics Chromatin / metabolism Chromatin Assembly and Disassembly Drosophila Proteins / genetics Drosophila melanogaster / genetics* Drosophila melanogaster / growth & development* Ecdysone / physiology Gene Deletion Gene Expression Profiling Gene Expression Regulation, Developmental Genes, Lethal* Histone Acetyltransferases / genetics Histone Acetyltransferases / metabolism* Larva / genetics Larva / metabolism Linear Models Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism* Oligonucleotide Array Sequence Analysis Pupa / metabolism Transcription Factors / genetics Transcription Factors / metabolism |
| IF | 4.015 |
| 引用数 | 35 |
| WOS 分野 | GENETICS & HEREDITY |
| リソース情報 | |
| ショウジョウバエ | 15433R-2 15433R-3 |