| Vol. 9 |
Osamu IDETA, Atsushi YOSHIMURA, Tadayuki MATSUMOTO, Hiroshi TSUNEMATSU and Nobuo IWATA
Plant Breeding Laboratory, Faculty of Agriculture, Kyushu University, Fukuoka, 812 Japan
To furnish significant information for genome analysis of rice, we have tried to integrate the conventional and RFLP linkage maps of chromosomes 1, 2, 3 and 4. Seven F2 populations were observed, which were between an Indica variety IR24 and Japonica marker lines with different genetic markers and 32 RFLP markers (Saito et al. 1991). The markers segregating in the F2 populations were as follows: chromosome 1, fs-2, d-18, rl-2, spl-6 and eg; chromosome 2, tri, bl-1 and spl-2; chromosome 3, chl-1, fc-1 and spl-3; and chromosome 4, d-11, Ph, lg and d-2. The RFLP markers without prefix XNpb are shown on the RFLP linkage map (Fig. .1). Some RFLP markers were found to be closely linked to morphological markers. In addition, a low degree of saturation was found out for the RFLP linkage map on the chromosomes 2 and 4. The RFLP map lacked the regions corresponding to tri and spl-2 on the chromosome 2 and the d-2 region on the chromosome 4 in the conventional linkage map, respectively.
Fig. 1. Arrangement of conventional, RFLP and integrated linkage maps on chromosomes 1, 2, 3 and 4 in rice. Numbers at the top indicate chromosomes. For each chromosome, the integrated map is shown in the center, the conventional linkage map (Chromosome 1: Iwata et al. 1989a, Chromosome 2: Iwata et al. 1989b, Chromosome 3: Iwata et al. 1984, Chromosome 4: Iwata and Omura 1971) is on the left and the RFLP linkage map (Saito et al. 1991) is on the right. The letters of prefix of the RFLP markers, XNpb, are omitted in this figure. The loci in ellipses are morphological markers used in this study, and in boxes are RFLP markers.
References
Iwata, N. and T. Omura, 1971. Linkage analysis by reciprocal translocation method in rice plants (Oryza sativa L.). II. Linkage groups corresponding to the chromosomes 5, 6, 8, 9, 10 and I 1. Sci. Bull. Fac. Agr., Kyushu Univ. 25: 137-153. (Japanese/English)
Iwata, N., H. Satoh and T. Omura, 1984. The relationships between chromosomes identified cytologically and linkage groups. RGN 1: 128-132.
Iwata, N., H. Satoh and A. Yoshimura, 1989a. Linkage studies in rice (Oryza sativa L.). Linkage map of chromosome 3. Bull. Inst. Trop. Agr., Kyushu Univ. 12: 1-9.
Iwata, N., H. Satoh and A. Yoshimura, 1989b. Linkage map for Nishimura's chromosome 8. RGN 6: 106-108.
Saito, A., M. Yano, N. Kishimoto, M. Nakagahra, A. Yoshimura, K. Saito, S. Kuhara, Y. Ukai, M. Kawase, T. Nagamine, S. Yoshimura, O. Ideta, R. Ohsawa, Y. Hayano, N. Iwata and M. Sugiura, 1991. Linkage map of restriction fragment length polymorphism loci in rice. Jpn. J. Breed. 41: 665-670.
| Vol. 9 |