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著者 Kim H.B., Cho J.I., Ryoo N., Shin D.H., Park Y.I., Hwang Y.S., Lee S.K., An G., Jeon J.S.
タイトル Role of rice cytosolic hexokinase OsHXK7 in sugar signaling and metabolism.
Abstract:
We characterized the function of the rice cytosolic hexokinase OsHXK7 (Oryza sativa Hexokinase7), which is highly upregulated when seeds germinate under O2 -deficient conditions. According to transient expression assays that used the promoter:luciferase fusion construct, OsHXK7 enhanced the glucose (Glc)-dependent repression of a rice alpha-amylase gene (RAmy3D) in the mesophyll protoplasts of maize, but its catalytically inactive mutant alleles did not. Consistently, the expression of OsHXK7, but not its catalytically inactive alleles, complemented the Arabidopsis glucose insensitive2-1 (gin2-1) mutant, thereby resulting in the wild type characteristics of Glc-dependent repression, seedling development, and plant growth. Interestingly, OsHXK7-mediated Glc-dependent repression was abolished in the O2 -deficient mesophyll protoplasts of maize. This result provides compelling evidence that OsHXK7 functions in sugar signaling via a glycolysis-dependent manner under normal conditions, but its signaling role is suppressed when O2 is deficient. The germination of two null OsHXK7 mutants, OsHXK7-1 and OsHXK7-2, was affected by O2 deficiency, but overexpression enhanced germination in rice. This result suggests the distinct role that OsHXK7 plays in sugar metabolism and efficient germination by enforcing glycolysis-mediated fermentation in O2 -deficient rice.
掲載誌 J Integr Plant Biol
Country South Korea
刊数
ページ
発刊年 2015
PubMed ID 25951042
PubMed Central ID -
DOI 10.1111/jipb.12366
URL -
関連情報
遺伝子 AMY3D HXK5 HXK6 HXK7
INSD -
系統 野生イネ
コアコレクション 		-
突然変異系統(遺伝研 Collection) -
種子不稔系統 -
致死変異体系統 -
組織別発生段階
- 突然変異系統(遺伝子) 		-
栽培イネ品種(遺伝研 Collection) -
組織別発生段階 -
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