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| 文献 | ||
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| 著者 | Morita K., Hatanaka T., Misoo S., Fukayama H. | |
| タイトル | Unusual Small Subunit, OsRbcS1, that is not Expressed in Photosynthetic Cells Alters the Catalytic Properties of Rubisco in Rice. | |
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Abstract: Rubisco small subunits (RbcS) are encoded by a nuclear multigene family in plants. Five RbcS genes (OsRbcS1-5) have been identified in rice (Oryza sativa). Among them, the amino acid sequence of OsRbcS1 differs notably from those of OsRbcS2-5. Phylogenetic analysis showed that OsRbcS1 is genetically distant from other rice RbcS and more closely related to RbcS from a fern and two woody plants. RT-PCR and promoter GUS analyses revealed that OsRbcS1 was not expressed in leaf blade, a major photosynthetic organ in rice, but was expressed in leaf sheath, culm, anther and root central cylinder. In leaf blade of transgenic rice overexpressing OsRbcS1 and leaf sheath of non-transgenic rice, OsRbcS1 was incorporated into the Rubisco holoenzyme. Incorporation of OsRbcS1 into Rubisco increased the catalytic turnover rate and Km for CO2 of the enzyme and slightly decreased the specificity for CO2, indicating that the catalytic properties were shifted to those of a high activity type Rubisco. The CO2 assimilation rate at low CO2 partial pressure was decreased in overexpression lines, but was not changed under ambient and high CO2 partial pressure compared to non-transgenic rice. Although the Rubisco content was increased, Rubisco activation state was decreased in overexpression lines. These results indicate that the catalytic properties of Rubisco can be altered by ectopic expression of OsRbcS1, with substantial effects on photosynthetic performance in rice. We believe this is the first demonstration of organ specific expression of individual members of the RbcS gene family resulting in marked effects on Rubisco catalytic activity. |
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| 掲載誌 | Plant Physiol. | |
| Country | unknown | |
| 刊数 | 164(1) | |
| ページ | 69-79 | |
| 発刊年 | 2014 | |
| PubMed ID | 24254313 | |
| PubMed Central ID | - | |
| DOI | 10.1104/pp.113.228015 | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | RBCS RBCS1 RBCS3 RBCS4 RBCS5 | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
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| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
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組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
