更新情報研究支援ツール - 文献 - 詳細
文献詳細
| 文献 | ||
|---|---|---|
| 著者 | Park S.H., Chung P.J., Juntawong P., Bailey-Serres J., Kim Y.S., Jung H., Bang S.W., Kim Y.K., Do Choi Y., Kim J.K. | |
| タイトル | Posttranscriptional Control of Photosynthetic mRNA Decay under Stress Conditions Requires 3' and 5' Untranslated Regions and Correlates with Differential Polysome Association in Rice. | |
|
Abstract: Abiotic stress, including drought, salinity, and temperature extremes, regulates gene expression at the transcriptional and posttranscriptional levels. Expression profiling of total messenger RNAs (mRNAs) from rice (Oryza sativa) leaves grown under stress conditions revealed that the transcript levels of photosynthetic genes are reduced more rapidly than others, a phenomenon referred to as stress-induced mRNA decay (SMD). By comparing RNA polymerase II engagement with the steady-state mRNA level, we show here that SMD is a posttranscriptional event. The SMD of photosynthetic genes was further verified by measuring the half-lives of the small subunit of Rubisco (RbcS1) and Chlorophyll a/b-Binding Protein1 (Cab1) mRNAs during stress conditions in the presence of the transcription inhibitor cordycepin. To discern any correlation between SMD and the process of translation, changes in total and polysome-associated mRNA levels after stress were measured. Total and polysome-associated mRNA levels of two photosynthetic (RbcS1 and Cab1) and two stress-inducible (Dehydration Stress-Inducible Protein1 and Salt-Induced Protein) genes were found to be markedly similar. This demonstrated the importance of polysome association for transcript stability under stress conditions. Microarray experiments performed on total and polysomal mRNAs indicate that approximately half of all mRNAs that undergo SMD remain polysome associated during stress treatments. To delineate the functional determinant(s) of mRNAs responsible for SMD, the RbcS1 and Cab1 transcripts were dissected into several components. The expressions of different combinations of the mRNA components were analyzed under stress conditions, revealing that both 3' and 5' untranslated regions are necessary for SMD. Our results, therefore, suggest that the posttranscriptional control of photosynthetic mRNA decay under stress conditions requires both 3' and 5' untranslated regions and correlates with differential polysome association. |
||
| 掲載誌 | Plant Physiol. | |
| Country | South Korea | |
| 刊数 | 159(3) | |
| ページ | 1111-24 | |
| 発刊年 | 2012 | |
| PubMed ID | 22566494 | |
| PubMed Central ID | 3387698 | |
| DOI | - | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | ABC1 ATASE3 CAB1R CABE CBSSIS1 CIN5 CP24 CP26 ENODL12 ENODL4 F2KP2 FKII GBSSII LHCB3 PFK6 PFPB PHO1 PKPBETA2 PPGM PSAA PSAD PSAK PSAN PSBX PSBY RBCS RBCS3 RCA SSIIB TCD9 UCL35 UCL4 YGDL1 YGL6 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
- |
| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
|
組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
