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| 著者 | Fukayama H., Ueguchi C., Nishikawa K., Katoh N., Ishikawa C., Masumoto C., Hatanaka T., Misoo S. | |
| タイトル | Overexpression of Rubisco Activase Decreases the Photosynthetic CO2 Assimilation Rate by Reducing Rubisco Content in Rice Leaves. | |
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Abstract: The effects of overexpression of Rubisco Activase on photosynthesis were studied in transgenic rice expressing barley or maize Rubisco Activase. Immunoblot and SDS-PAGE analyses showed that transgenic lines from both gene constructs expressed the foreign Rubisco Activase at high levels. The activation state of Rubisco in transgenic lines was slightly higher than that in non-transgenic plants (NT). In addition, light activation of Rubisco was significantly more rapid in transgenic lines compared with NT. These findings indicate that the overexpression of Rubisco Activase can enhance Rubisco activation. However, despite enhanced activation of Rubisco in these transgenic plants, CO(2) assimilation rate at ambient CO(2) conditions was decreased. This decrease in CO(2) assimilation rate was observed in both young developing and mature leaves independent of N nutrition. The contents of nitrogen and chlorophyll did not differ significantly between transformants and NT, however, Rubisco content was substantially decreased in transgenic lines. There was no evidence for reduced transcription of RbcS or RbcL in these transgenic lines, in fact transcript levels were marginally increased compared to NT. These results indicate that the overexpression of Rubisco Activase leads to a decrease in Rubisco content, possibly due to post-transcriptional mechanisms. |
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| 掲載誌 | Plant Cell Physiol. | |
| Country | Japan | |
| 刊数 | 53(6) | |
| ページ | 976-86 | |
| 発刊年 | 2012 | |
| PubMed ID | 22470057 | |
| PubMed Central ID | - | |
| DOI | - | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | RBCL RBCS RBCS1 RBCS3 RBCS4 RBCS5 RCA | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
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| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
|
組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
