The following rice genetic resources possessed by our laboratory are ready to open to the public for seed and information distribution.
  Seed distribution will be done according to the MTA. Please request the material after carefully reading the conditions stated in MTA.
  List of experimental strains indicates the availability of seed material in the column named as distribution. If it is an "ok", the seed is available. For the strains obtained from other organization, the strains of low amount of seed stock or those which are under research work in our laboratory at present, it is left blank, indicating that they are not available for distribution.
  If you have any questions or you find any errors in the list, please contact the person in charge for the experimental strain group indicated in the "Outline of Rice Experimental Strain Groups" page.

1. List Of Strains

Family Name	Outline	Count
SG	Stocks for genes	194 Strains	Please refer to Rice Mutant Mapping Database about the gene mapping position.
FL	F Lines	588 Strains	Please refer to Rice Mutant Mapping Database about the gene mapping position.
Fn(T65)	Backcross Fn generation (T65)	287 Strains	Please refer to Rice Mutant Mapping Database about the gene mapping position.
RT	Reciprocal Translocation	288 Strains
RIA	Recombinant Inbred Lines A	83 Strains	These are strains currently opened to the public from before under the family name "KRIL".（Rice Genetics Newsletter 12:154(1995)）． Genotype of each strain can be viewed http://rgp.dna.affrc.go.jp/dnabank/publicdata/asoir24 /readme.html.
IAS	Chromosome Substitution Lines (genetic background is "IR24")	70 Strains	Please refer to IAS Genotype List
AIS	Chromosome Substitution Lines (genetic background is "asominori")	98 Strains	Please refer to AIS Genotype List
GILs	Chromosome segment substitution lines( O. glaberrima introgression line)	41Strains	Please refer to GILs Genotype List
glumILs[T65]	Chromosome Substitution Lines (O. glumaepatula introgression line (T65 cytoplasm)	34Strains	Please refer to glumILs[T65] Genotype List
glumILs[glum]	Chromosome Substitution Lines (O. glumaepatula introgression line (glumaepatula cytoplasm))	35Strains	Please refer to glumILs[glum] Genotype List
merILs[T65]	Chromosome Substitution Lines (O. meridionalis introgression line (T65 cytoplasm))	42Strains	Please refer to merILs[T65] Genotype List
merILs[mer]	Chromosome Substitution Lines (O. meridionalis introgression line (meridionalis cytoplasm))	36Strains	Please refer to merILs[mer] Genotype List

  Details of each strain group are presented as follows:

SG Strain Group or "Stock for genes"
	Person in charge: Professor Atsusi Yoshimura
	Category: Marker gene stocks
	Genetic background: japonica cultivars
	No. of strains: 194
	Use: They are used for gene mapping.
	Origin & building procedure : They are based on the marker gene strains which have been used in our laboratory and the strains used for RFLP mapping of genes within these ten years.
	Features: They maintain original strains as possible. Most genetic loci are known for the position on RFLP linkage map.
	Reference: Yoshimura, A., O.Ideta and N. Iwata (1997) Linkage map of phenotype and RFLP markers in rice. Plant Mol. Biol. 35:49-60.
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FL Strain Group or "F lines"
	Person in charge: Professor A. Yoshimura
	Category: Marker gene accumulation stocks
	Genetic background: japonica cultivars
	No. of strains: 588
	Use: These strains have been used for "Mapping of rice genes", which is the main research theme of Plant Breeding Laboratory.
	Origin & building procedure : From the offspring of hybridization between marker strains, the plants having multiple markers were chosen and self-propagated at least to the F16 generation.
	Features: More than two markers are included.
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Fn(T65) Strain Group or "Backcross Fn generation (T65)"
	Person in charge: Professor A. Yoshimura
	Category: Marker gene stocks
	Genetic background: japonica cultivar "Taichung 65"
	Donor parent: Mainly FL stocks
	No. of strains: 287
	Use: Gene mapping and a tester for various genetic experiments. "Taichung 65" is convenient material for the transformation experiment as its efficiency for callus differentiation and redifferentiation. Origin & building procedure: F1 between Taichung 65 and FL strain was backcrossed to Taichung 65.
	Features: Uniformity of heading date is useful for genetic experiments.
	Reference: Yoshimura, A., O. Ideta and N. Iwata (1997) Linkage map of phenotype and RFLP markers in rice. Plant Mol. Biol. 35: 49-60.
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Triplo Strain Group
	Person in charge: Dr. Hideshi Yasui
	Category : Trisomics
	Origin :	Progeny of autotriploids x diploid ("Nipponbare") Progeny of desynapsis mutant x diploid ("Kinmaze” and "Taichung 65")
	Use: Belonging chromosome is determined for marker genes and DNA markers.
	Construction procedure: (1) From the progeny of hybridization between autotriploid and diploid of "Nipponbare", trisomics having various morphological characteristics were chosen and trisomic analysis was performed by crossing to marker gene strains. Primary trisomic series with genetic background of "Nipponbare" was constructed. (2) Desynaptic variants induced by MNU treatment on fertilized eggs of "Kinmaze" and "Taichung 65" were crossed to their diploid parent, and from their progeny, primary trisomics series having each variety as genetic background were established based on the morphological characteristics.
	Features of strains: Distinctive characteristics of grain shape, panicle shape and plant type are associated with the concerning extra chromosome or area of extra chromosome.
	Reference: Iwata, N., and Omura, T. 1984. Studies on the trisomics in rice plants (Oryza sativa L.). VI. An accomplishment of a trisomic series in japonica rice plants. Japan. J. Genet. 59: 199-204. Yasui, H., H. Satoh and N. Iwata 1989. Establishment of a trisomic series in rice by using a desynaptic mutant. Rice Genetics Newsletter 6: 50-51. Nonomura, K. I., A. Yoshimura and N. Iwata 1997. Cytogenetical gene mapping by reciprocal translocation and tertiary trisomic analyses in rice (Oryza sativa L.). Genes Genet. Syst. 72:41-49.
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RT (Reciprocal Translocation) Strains
	Person in charge: Professor A. Yoshimura
	Genetic background: japonica cultivars
	No. of strains: 288
	Use: These strains have been used for the source of various aneuploids and the materials of gene mapping. It is expected to be useful for a material of chromosome research.
	Origin : (1) RT strains identified in X-rayed and atomic bomb induced variants by Dr. Y. Nishimura: 29 strains. (2) RT strains chosen from the atomic bomb induced rice variant progeny and identified for their participant chromosomes in our Laboratory: 9 strains. (3) RT strains obtained from Dr. H.I. Oka and identified for their participant chromosomes in our Laboratory: 18 strains. (4) RT strains obtained from the progeny of gamma ray (Co60) treated "Taichung 65" and "Nipponbare": 195 strains among which 38 strains are identified for their participant chromosomes. (5) Isogenic RT strains having the "Taichung 65" background obtained from Dr. S. Sato, Ryukyu University: 40 strains.
	Features: Many RT strains are identified for their participating chromosomes. Among them, the strains originated from the atomic bomb induced variants are of historical significance.
	Reference: Yoshimura, A., N. Iwata, T. Kawasaki, M. Ali and T. Omura (1988) Identification of interchanged chromosomes in twenty-five reciprocal translocation lines newly established in rice cultivar Nipponbare. Rice Genetics Newsletter 5: 62-64.
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RIA (Recombinant Inbred Lines A)
	Person in charge: Professor Atsusi Yoshimura
	Category: Recombinant inbred lines.
	No. of strains: 83
	Use: In our laboratory, they were used for detection of quantitative trait loci (QTL), and mapping of genes and DNA clones.
	Origin & construction procedure : From the progeny of the cross between "Asominori" (japonica) and "IR24" (indica), selfing generation has been advanced by Single Seed Decent Method (SSD) to the F12 generation at present.
	Features: RIA strains are genetically homozygous and can be used 7 for QTL analysis. RIA is useful in linkage analysis for synthesizing map information and determining map position of newly isolated gene or molecular marker. These strains are available for distribution at present.
	Linkage map : The analysis based on 71 strains of the F6 and F7 generations determined the position of 375 RFLP markers. The constructed linkage map covers almost the entire rice genome, the total map distance being 1275cM.
	QTL analysis : Various QTL analyses have been done on this material. Based on the results, further details are being analyzed using IAS and AIS strains.
	Reference: Tsunematsu, H, A. Yoshimura, Y. Harushima, Y. Nagamura, N. Kurata, M. Yano, T. Sasaki and N. Iwata (1996) RFLP Framework map using recombinant inbred lines in rice. Breeding Science 46:279-284.
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RIB Strain Group (Recombinant Inbred Lines B)
	Person in charge: Professor Atsusi Yoshimura
	Category: Recombinant inbred lines.
	Origin: "Kinmaze" (japonica) x "DV85" (indica)
	No. of strains: 81
	Use : Detection of QTL in japonica and indica cultivars.
	Construction procedure : Based on the progeny of the crossing between "Kinmaze" and "DV85", selfing generation has been advanced by SSD method to the F12 generation at present.
	Linkage map : Map positions of 138 RFLP markers have been determined. The constructed linkage map covers almost entire rice genome, the total map distance being 1332cM.
	QTL analysis : Various QTL analyses are being performed on this material.
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RIC Strain Group (Recombinant Inbred Lines C)
	Person in charge:Dr. H. Yasui
	Category: Recombinant inbred lines.
	Origin: "Taichung 65" (japonica) x "DV85" (indica)
	No. of strains: 123
	Use: Detection of QTL in japonica and indica cultivars.
	Construction procedure : Based on the progeny of the crossing between "Taichung 65" and "DV85", selfing generation has been advanced by SSD method to the F12 generation at present.
	Linkage map : Using 123 strains of the F9 generation, map positions of 110 RFLP markers have been determined.
	QTL analysis : Various QTL analyses are being performed in our Laboratory.
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RID Strain Group (Recombinant Inbred Lines D)
	Person in charge: Dr. H. Yasui
	Category: Recombinant inbred lines.
	Origin: "Taichung 65" (japonica) x "ARC10313" (indica)
	No. of strains: 136
	Use: Detection of QTL in japonica and indica cultivars.
	Construction procedure : Based on the progeny of the crossing between “Taichung 65” and “ARC10313”, selfing generation has been advanced by SSD method to the F12 generation at present.
	Linkage map :Using 136 strains of the F10 generation, map positions of 88 RFLP markers have been determined.
	QTL analysis : Various QTL analyses are being performed in our Laboratory.
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IAS Strain Group
	Person in charge: Professor Atsusi Yoshimura
	Category: Chromosome segment substitution line
	Genetic background: "IR24" (indica)
	Donor parent: "Asominori" (japonica)
	No. of strains: 70
	Use: Detailed QTL analysis, gene mapping and isolation, analysis of gene interaction.
	Construction procedure : In each backcross generation of 18 RIA strains to "IR24", RFLP marker selection was performed to establish chromosome segment substitution line. Substitution chromosome segments of donor parent “Asominori”, overlapping among strains, covers entire genome. From 888 candidate plants chosen at BC2F2 generation by marker selection, finally 70 chromosome segment substitution lines were established. They have been kept by selfing and reached to BC2F7 generation at present.
	Features: IAS strains cover almost entire genome area with substitution segment of "Asominori" chromosome and their genetic background became essentially that of "IR24", recurrent parent. They are efficient experimental populations for the analysis of traits controlled by multiple genes. They may be used for construction of NIL and map based cloning. Using this group, principal genes concerning heading date has been identified. IAS is expected, together with its reciprocal counterpart AIS, to be used effectively for gene interaction analysis.
	Reference: Kubo, T., Y. Aida, K. Nakamura, H. Tsunematsu, K. Doi and A. Yoshimura (2002) Reciprocal chromosome segment substitution series derived from Japonica and Indica cross of rice (Oryza sativa L.). Breed. Sci. 52:319-325.
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AIS Strain Group
	Person in charge: Professor Atsusi Yoshimura
	Category: Chromosome segment substitution line
	Genetic background: "Asominori" (japonica)
	Donor parent: "IR24" (indica)
	No. of strains: 98
	Use: Detailed QTL analysis, gene mapping and isolation, analysis of gene interaction.
	Construction procedure : In the fourth backcross generation to "Asominori", BC3F1, and its selfing generation, BC3F2, RFLP marker selection was performed to establish chromosome segment substitution line. Substitution chromosome segments of donor parent "IR24", overlapping among strains, covers entire genome. Candidate plants were chosen in respect of uniformity of background. In BC3F1 generation 66 plants were chosen based on 116 RFLP markers. From their 400 selfed progeny plants, finally 91 chromosome segment substitution lines were established. They have been kept by selfing and reached to BC3F6 generation at present.
	Features: AIS strains cover almost entire genome area except a part of Chromosome 3 with substitution segment of "IR24" chromosome and their genetic background became essentially that of "Asominori", recurrent parent. They are efficient experimental populations for the analysis of traits controlled by multiple genes. They may be used for construction of NIL and map based cloning. Using this material, principal genes concerning hybrid sterility have been identified. AIS is expected, together with its reciprocal counterpart IAS, to be used effectively for gene interaction analysis.
	Reference: Kubo, T., Y. Aida, K. Nakamura, H. Tsunematsu, K. Doi and A. Yoshimura (2002) Reciprocal chromosome segment substitution series derived from Japonica and Indica cross of rice (Oryza sativa L.). Breed. Sci. 52:319-325.
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GILs Strain Group (O. glaberrima Introgression Lines)
	Person in charge: Dr. K. Doi
	Genetic background: "Taichung 65" (japonica)
	Donor parent: O. glaberrima (IRGC 104038)
	No. of strains: 41
	Use: Analysis of major traits characterizing O. glaberrima, detailed QTL analysis, gene mapping and isolation, analysis of gene interaction, etc.
	Construction procedure : BC3F1 was obtained by backcrossing of F1 to "Taichung 65" three times. From BC3F1 and its selfed progeny BC3F2, chromosome segment introgression lines was established by marker selection. Substitution chromosome segments of donor parent O. glaberrima, overlapping among lines, cover entire genome. Strains have been kept by selfing and reached to BC3F7 at present.
	Features: GILs successfully introduced chromosome segments originating in O. glaberrima to "Taichung 65", except a part of Chromosomes 1,7, 5, 10 and 12. The QTL analysis of major traits of O. glaberrima is in progress now. Heading date genes and hybrid pollen sterility genes have been clearly identified and located on the genetic map.
	Reference: Doi, K., N. Iwata and A. Yoshimura (1997) The construction of chromosome substitution lines of African rice (Oryza glaberrima Steud.) in the background of Japonica rice (O. sativa L.). Rice Genetics Newsletter 14 :39-41. Doi, K., A. Yoshimura and N. Iwata (1998) RFLP mapping and QTL analysis of heading date and pollen sterility using backcross populations between Oryza sativa L. and O. glaberrima Steud. Breed. Sci. 48: 395-399.
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glumILs Strain Group (O. glumaepatula Introgression Lines)
	Person in charge:Prof. A. Yoshimura
	Genetic background: "Taichung 65" (japonica)
	Donor parent: O. glumaepatula (IRGC 105668)
	No. of strains: (1) T65 cytoplasm series: 34     (2) glumaepatula cytoplasm series: 35
	Use: Analysis of major traits specific to O. glumaepatula, detailed QTL analysis, gene mapping and isolation, analysis of gene interaction, etc.
	Construction procedure : BC4F1 was obtained by backcrossing each of reciprocal F1’s of T65 x O. glumaepatula to pollen parent "Taichung 65" repeatedly. In BC3F1 and BC4F1, candidate plants were chosen by whole genome selection using 106 RFLP markers. In total, 72 lines were selected for T65 cytoplasmic series and 63 lines for O. glumaepatula cytoplasmic series.
	Features: glumILs covers almost entire nuclear genome of O. glumaepatula in both cytoplasmic groups, though there are some lacks in chromosomal area. There is strong hybrid weakness found in O. glumaepatula cytoplasm, but the introduction of weakness recovery gene (Rhw) on Chromosome 8 makes the maintenance of lines possible. In the process of glumILs establishment, gene loci were identified for several seed shattering and heading date genes. They benefit the gene analysis of specific traits to O. glumaepatula. These strains may be useful to analyze the cytoplasmic genomes as they have both of different cytoplasms.
	Reference Sobrizal, K. Ikeda, P. L. Sanchez, K. Doi, E. R. Angeles, G. S. Khush and A. Yoshimura (1999) Development of Oryza glumaepatula introgression lines in rice, Oryza sativa L. Rice Genetics Newsletter 16:107-108.
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merILs Strain Group (O. meridionalis Introgression Lines)
	Person in charge:Prof. A. Yoshimura
	Genetic background: "Taichung 65" (japonica)
	Donor parent: O. meridionalis (W1625)
	No. of strains: (1) T65 cytoplasm series: 42    (2) meridionalis cytoplasm series:36
	Use: Analysis of major traits specific to O. meridionalis , detailed QTL analysis, gene mapping and isolation, analysis of gene interaction, etc.
	Construction procedure : BC4F1 was obtained by backcrossing each of reciprocal F1’s of T65 x O. meridionalis to pollen parent “Taichung 65” repeatedly. In BC4F1, candidate plants were chosen by whole genome selection using 110 RFLP markers. In total, 26 lines were selected for T65 cytoplasmic series and 35 lines for O. glumaepatula cytoplasmic series.
	Features: merILs covers almost entire nuclear genome of O. meridionalis in both cytoplasmic groups, though there are some lacks in chromosomal area. In the process of glumILs establishment, gene loci were identified for several awned and shattering genes. These strains may be useful to analyze the cytoplasmic genomes as they have both of different cytoplasms.
	Reference Kurakazu, T., Sobrizal, K. Ikeda, P. L. Sanchez, K. Doi, E. R. Angeles, G. S. Khush and A. Yoshimura (2001) Oryza meridionalis chromosomal segment introgression lines in cultivated rice, O. sativa L. Rice Genetics Newsletter 18:81-82.
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2. The MTA form for rice seed distribution

BIOLOGICAL MATERIAL TRANSFER AGREEMENT (pdf File)

3. Outline of the rice genetic stock reservation project

	Outline of the rice genetic stock reservation project
	This document states the outline of management and development of rice genetic resources being performed in our laboratory.

4. Rice mutant mapping database

The results of mutant locus mapping on the RFLP map so far achieved in our laboratory and the morphological pictures of mutants are presented in http://www.agr.kyushu-u.ac.jp/agpm/ikushu/mutant/.