OK-Cab^lf

Strain information
Strain ID MT1335
Strain Name OK-Cab^lf
Strain Type Mutants
Symbol lf
Characteristics This mutant strain has a 14kb deletion in slc2a15b/lf gene induced by a CRISPR/Cas system, which lacks visible leucophores throughout life. The phenotype is similar to leucophore-free (lf) mutant (MT76), but its genetic background is OK-Cab (MT830). Observing the fluorescence using a GFP longpass filter makes it easy to see if there are any leucophores.
Journal
Category Mutants created by genome editing
Organization National Institute for Basic Biology
Live
Deposited by National Institute for Basic Biology
Order Request
Image

MT1335_OK-Cab^lf_embryo
The Cab^lf strain has no auto-fluorescence from leucophores, but the original cab strain has strong auto-fluorescence. The Cab^lf is useful for establishing the transgenic line with fluorescence proteins.
Image

MT1335_OK-Cab^lf_fry

Document (PDF) MT1335_OK-Cab^lf_genotyping protocol


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