|
LCE^mAzamiGreen
| Strain information | |
|---|---|
| Strain ID | TG1566 |
| Strain Name | LCE^mAzamiGreen |
| Strain Type | Transgenics |
| Characteristics | A knock-in strain by intergrading a transgenic construct containing Linker-mAzamiGreen-SV40polyA into the sixth intron of the lce (low choriolytic enzyme) gene using CRISPR/Cas system. The green fluorescent protein (GFP) mAzami-Green is fused to the C-terminal end of the hatching enzyme protein LCE. At the later develeopmental stages, GFP expression is observed in the hatching gland. |
Journal
|
|
| Category | Transgenic strains |
| Organization | National Institute for Basic Biology |
| Frozen sperm in NIBB | ○ |
| Deposited by | NIBB |
| Order |
|
| Image |
pUC-LCE_exon8+9-mAG_.jpg lce_GenomicSeq-C-terminal_Linker-mAG-SV40pA in pUC19 |
| Image |
LCE_deposit.jpg GFP expression pattern in the embryos of knock-in strain LCELinker-mAG from the ventral (left) and frontal (right) views. Dotted lines indicate the positions of eyes. |
