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FSI-Tg(Gt I)-nab2
| Strain information | |
|---|---|
| Strain ID | TG1339 |
| Strain Name | FSI-Tg(Gt I)-nab2 |
| Strain Type | Transgenics |
| Characteristics |
Single transgene integration on chromosome 5, EGFP fluorescence in CNS and rhombomeres. See: Froschauer, A., Sprott, D., Gerwien, F. et al. Effective generation of transgenic reporter and gene trap lines of the medaka (Oryzias latipes) using the Ac/Ds transposon system. Transgenic Res 21, 149.162 (2012). https://doi.org/10.1007/s11248-011-9514-x Transgene: minDS-Sites of Ac/Ds transposon vector pMDS6 flanking the third intron/fourth exon (including splice donor and acceptor sites) of the medaka actb gene. Exon4 fused in frame to EGFP and SV40 polyadenylation site (Clontech) . For pMDS6 see: Emelyanov et al (2006) Transkingdom transposition of the maize dissociation element. Genetics 174(3):1095.1104 |
Journal
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| Special affairs [Address] |
■ The RECIPIENT shall refer the following publication in any PUBLICATIONS. (Name of the Publication: FROSCHAUER, Alexander, et al. Effective generation of transgenic reporter and gene trap lines of the medaka (Oryzias latipes) using the Ac/Ds transposon system. Transgenic research, 2012, 21.1: 149-162. ) |
| Category | Transgenic strains |
| Organization | National Institute for Basic Biology |
| Frozen sperm in NIBB | ○ |
| Deposited by | Technische Universitaet Dresden |
| Order |
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| Document (PDF) | Tg(GT I)_transgene map |
| Image |
TG1339_Tg(Gt I)-nab2 Characteristic embryonic fluorescence patterns in the gene trap line FSI-Tg(GtI)-nab2. The nab2-GtI pattern is characterized by a weak ubiquitous expression and an intensive stripe (prospective rhombomere 3; arrowhead) in the hindbrain at 1 dpf (A). Two stripes likely corresponding to the rhombomeres 3 and 5 are visible from 2 dpf (b) until 3 dpf (c). |
| Document (PDF) | Genotyping protocol_TG1339_FSI-Tg(GtI)-nab2 |
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