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mpz-EGFP
| Strain information | |
|---|---|
| Strain ID | TG1358 |
| Strain Name | mpz-EGFP |
| Strain Type | Transgenics |
| Characteristics | mpz-EGFP transgenic medaka line marks mpz positive mielinating cells. |
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| Special affairs [Address] |
■ The RECIPIENT shall give proper credit to the DEVELOPER in any publications reporting results of research using any derivatives developed from the BIOLOGICAL MATERIALS (hereinafter referred to as “the DERIVATIVES”), and shall procure other institutionsto which the DERIVATIVES are provided (including when provided through the NBRP) to do the same. |
| Category | Transgenic strains |
| Organization | National Institute for Basic Biology |
| Frozen sperm in NIBB | ○ |
| Deposited by | Showa University |
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| Image |
TG1358_mpz-EGFP_transgene map To produce mpz promoter-EGFP transgenic (mpz-EGFP) medaka, we amplified the 2.9-kb upstream regulatory region of the medaka mpz gene using the mpz promoter Sal I forward primer 5′- GTCGACTGGCTTCCATTCTTGACTCC-3′ and mpz promoter Kpn I reverse primer 5′-GGTACCGGTTGTTCCTGGCTTCCCAA-3′. The amplified fragment was cloned into a TA cloning vector (pCR-TOPO Vector, Thermofisher). We then digested the fragment with Sal I and Kpn I, and subcloned it into the Sal I and Kpn I sites of the I-SceI backbone vector, in which EGFP Express/SV40 polyA (Clontech) was inserted into the Hind III and AflII sites of pBSKI2 (Amagen). The Ensemble Genome database indicated the mpz gene in Japanese medaka fish HdrR. |
| Image |
TG1358 MPZ-GFP EGFP is expressed in brain, spinal cord, lateral line and around neurons at young adult stage. |
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