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FLFII-Tg(actb-EGFP)19
| Strain information | |
|---|---|
| Strain ID | TG1337 |
| Strain Name | FLFII-Tg(actb-EGFP)19 |
| Strain Type | Transgenics |
| Characteristics |
Single transgene integration on chromosome 19, strong ubiquitous EGFP fluorescence. Paternal expression in blastula visible. Maternal expression visible until 6 dpf (Iwamatsu stage 36) also in non-transgenic offspring. see: Froschauer, A., Sprott, D., Gerwien, F. et al. Effective generation of transgenic reporter and gene trap lines of the medaka (Oryzias latipes) using the Ac/Ds transposon system. Transgenic Res 21, 149.162 (2012). https://doi.org/10.1007/s11248-011-9514-x Transgene: minDS-Sites of Ac/Ds transposon vector pMDS6 flanking the actb-promoter (including intron1) and actb-UTR of medaka driving EGFP of Clontech. For pMDS6 see: Emelyanov et al (2006) Trans-Kingdom transposition of the maize dissociation element. Genetics 174(3):1095.1104 |
| Notes | Please note that we will use OK-Cab (MT830) for artificially insemination with frozen sperm of this strain. |
Journal
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| Special affairs [Address] |
■ The RECIPIENT shall refer the following publication in any PUBLICATIONS. (Name of the Publication: FROSCHAUER, Alexander, et al. Effective generation of transgenic reporter and gene trap lines of the medaka (Oryzias latipes) using the Ac/Ds transposon system. Transgenic research, 2012, 21.1: 149-162. ) |
| Category | Transgenic strains |
| Organization | National Institute for Basic Biology |
| Frozen sperm in NIBB | ○ |
| Deposited by | Technische Universitaet Dresden |
| Order |
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| Document (PDF) | Genotyping protocol_TG1337_FLFII-Tg(actb-EGFP)19 |
| Image |
TG1337_actb-EGFP Establishment of actb-EGFP transgenic medaka. a,b Embryos at 6 dpf (stage 36). Embryos were injected with plasmid DNA alone (a) or with plasmid DNA and transposase mRNA (b) at the 1.2-cell-stage. EGFP-expressing individuals were raised and mated to non-transgenic fish. c Zygotic transgene expression in an F1 embryo of a male founder begins at early blastula stage. d At hatching stage, the strongest expression is seen in the central nervous system and the digestive tract. |
| Document (PDF) | Tg(actb-EGFP)_transgenemap |
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