A knock-in strain by intergrading a transgenic construct containing Linker-mAzamiGreen-SV40polyA into the sixth intron of the lce (low choriolytic enzyme) gene using CRISPR/Cas system. The green fluorescent protein (GFP) mAzami-Green is fused to the C-terminal end of the hatching enzyme protein LCE. At the later develeopmental stages, GFP expression is observed in the hatching gland.
Journal
Category
Transgenic strains
Organization
National Institute for Basic Biology
Frozen sperm in NIBB
○
Deposited by
NIBB
Order
Image
pUC-LCE_exon8+9-mAG_.jpg
lce_GenomicSeq-C-terminal_Linker-mAG-SV40pA in pUC19
Image
LCE_deposit.jpg
GFP expression pattern in the embryos of knock-in strain LCELinker-mAG from the ventral (left) and frontal (right) views. Dotted lines indicate the positions of eyes.