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runx2-EGFP
| Strain information | |
|---|---|
| Strain ID | TG1397 |
| Strain Name | runx2-EGFP |
| Strain Type | Transgenics |
| Characteristics | runx2(RUNX family transcription factor 2) is a transcription factor of the runt family and acts as a key regulator in skeletal development. A BAC clone (ola1-019D18) including the runx2 gene, which was identified from the Medaka ensemble database, was used for homologous recombination. The EGFP-kanamycin cassette was recombined into exon 1 of the medaka runx2 gene. The medaka Cab line was used for establishment of the runx2-EGFP transgenic medaka. The EGFP expression is observed in chondrocytes and immature osteoblasts. |
Journal
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| Special affairs [Address] |
■ The RECIPIENT shall refer the following publication in any PUBLICATIONS. (Name of the Publication: Azetsu, Y., Inohaya, K., Takano, Y., Kinoshita, M., Tasaki, M., & Kudo, A. (2017). The sp7 gene is required for maturation of osteoblast-lineage cells in medaka (Oryzias latipes) vertebral column development. Developmental biology, 431(2), 252-262.) |
| Category | Transgenic strains |
| Organization | National Institute for Basic Biology |
| Frozen sperm in NIBB | ○ |
| Deposited by | Tokyo Institute of Technology |
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| Image |
TG1397_runx2GFP EGFP-expression is observed in the skeletal elements. (A) Ventral view of head region of a just hatched larva. (B) Lateral view of caudal fin of a just hatched larva. (C) Lateral view of tail region of ca. 6 mm larva. na, neural arch; ha, hemal arch. |
| Document (PDF) | Transgenemap_TG1397_runx2GFP |
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