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FSI-Tg(Gt I)-gpx7
| Strain information | |
|---|---|
| Strain ID | TG1338 |
| Strain Name | FSI-Tg(Gt I)-gpx7 |
| Strain Type | Transgenics |
| Characteristics |
Single transgene integration on chromosome 4, EGFP fluorescence in CNS and weak in all tissues. Expression nearly ubiquitous at 1 dpf. Line not published, for transgene see https://doi.org/10.1007/s11248-011-9514-x Transgene: minDS-Sites of Ac/Ds transposon vector pMDS6 flanking the third intron/fourth exon (including splice donor and acceptor sites) of the medaka actb gene. Exon4 fused in frame to EGFP and SV40 polyadenylation site (Clontech) For pMDS6 see: Emelyanov et al (2006) Trans-Kingdom transposition of the maize dissociation element. Genetics 174(3):1095.1104 |
Journal
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| Special affairs [Address] |
■ The RECIPIENT shall refer the following publication in any PUBLICATIONS. (Name of the Publication: FROSCHAUER, Alexander, et al. Effective generation of transgenic reporter and gene trap lines of the medaka (Oryzias latipes) using the Ac/Ds transposon system. Transgenic research, 2012, 21.1: 149-162. ) |
| Category | Transgenic strains |
| Organization | National Institute for Basic Biology |
| Frozen sperm in NIBB | ○ |
| Deposited by | Technische Universitaet Dresden |
| Order |
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| Document (PDF) | Tg(GT I)_transgene map |
| Image |
TG1338_Tg(GtI)-gpx7_1dpf_2dpf 1 dpf top view (left) and 2 dpf (middle and right); Fluorescence with longpass filter set gives yellow yolk fluorescence |
| Document (PDF) | Genotyping protocol_TG1338_FSI-Tg(GtI)-gpx7 |
