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Tg(fli1:DsRed)
| Strain information | |
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| Strain ID | TG1361 |
| Strain Name | Tg(fli1:DsRed) |
| Strain Type | Transgenics |
| Characteristics | Friend leukemia integration 1 transcription factor (fli1), known as transcription factor ERGB. fli1:DsRed transgenic medaka line marks fli1 positive endothelial cells. You can see the angiogenesis during fin ray bone fracture healing by using this line (Takeyama et al Dev Biol 2014). |
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| Special affairs [Address] | ■ The RECIPIENT shall give proper credit to the DEVELOPER in any publications reporting results of research using any derivatives developed from the BIOLOGICAL MATERIALS (hereinafter referred to as “the DERIVATIVES”), and shall procure other institutionsto which the DERIVATIVES are provided (including when provided through the NBRP) to do the same. |
| Category | Transgenic strains |
| Organization | National Institute for Basic Biology |
| Frozen sperm in NIBB | ○ |
| Deposited by | Showa University |
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| Image |
TG1361_Tg(fli1DsRed)_transgene map For creating the fli1 promoter DsRed transgenic medaka, Tg(fli1:DsRed), the 4.6-kb upstream region of the medaka fli1 gene was amplified by using the following primers: fli1 promoter F-XhoI, 5-TGTGTCCTGAATGTCCTGAC-3; and fli1 promoter R-KpnI,5-TTTGCGCAAATTTTCGCGGACC-3. The digested fragment with XhoI and KpnI was subcloned into the XhoI/KpnI site of the I-SceI backbone vector, in which MCS/DsRed Express/SV40polyA of pDsRed Express (Clontech) was inserted into HindIII and AflII sites of PBSKI2 (AMAGEN). |
| Image |
TG1361Tg(fli1 DsRed) DsRed is expressed in vascular endothelial cells in larva. |
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