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TRAP-DsRed
| Strain information | |
|---|---|
| Strain ID | TG1362 |
| Strain Name | TRAP-DsRed |
| Strain Type | Transgenics |
| Characteristics | TRAP-DsRed transgenic medaka line marks TRAP positive osteoclast. |
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| Special affairs [Address] |
■ The RECIPIENT shall give proper credit to the DEVELOPER in any publications reporting results of research using any derivatives developed from the BIOLOGICAL MATERIALS (hereinafter referred to as “the DERIVATIVES”), and shall procure other institutionsto which the DERIVATIVES are provided (including when provided through the NBRP) to do the same. |
| Category | Transgenic strains |
| Organization | National Institute for Basic Biology |
| Frozen sperm in NIBB | ○ |
| Deposited by | Showa University |
| Order |
Under preparation for distribution |
| Image |
TG1362_TRAP-DsRed_transgene map A 3-kb upstream regulatory region of the medaka TRAP gene was amplified using the primers TRAP pro F-HindIII, 5′-CCCAAGCTTGGGTCGCACTCTGGATACAACAGC-3′,and TRAP proR-SacII U,5′-CCGCGGTTCAACTGAAGCAACACACC-3′. The amplified fragment was cloned into the TA cloning vector. We then digested the 5′-half fragment with HindIII and SacIIU and inserted the I-SceI backbone vector, which was inserted into DsRed-Express/SV40polyA (Clontech #632412) at the HindIII/AflII sites in pBSKI2 vector(AMAGEN) |
| Image |
TG1362 TRAP-DsRed DsRed is expressed in osteoclast around the bone. |
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