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sox5^ml-3/ml-3;TgBAC(pax7a:loxp-dsred-loxp-sox5^WT-2A-venus)
| Strain information | |
|---|---|
| Strain ID | TG1210 |
| Strain Name | sox5^ml-3/ml-3;TgBAC(pax7a:loxp-dsred-loxp-sox5^WT-2A-venus) |
| Strain Type | Transgenics |
| Characteristics |
DsRed fluorescence is driven by pax7a promoter. Once recombination is induced by cre at the loxp sites, sox5 (WT) becomes expressed there. For the purpose of establishing this strain, TgBAC(pax7a:loxp-dsred-loxp-sox5^WT-2A-venus) DNA was injected into embryos of ml-3 mutant with B/B (having normally-melanized melanophores), which had been obtined by crossing ml-3 original b/b mutant (not having normally-melanized melanophores, since b allele is a recessive loss-of-function mutant allele) with Nagoya (B/B). Thus this strain has copies of TgBAC transgene as well as dominant B alleles. |
| Notes | Please note that we will use OK-Cab (MT830) for artificially insemination with frozen sperm of this strain. |
Journal
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| Special affairs [Address] |
■ The RECIPIENT shall refer the following publication in any PUBLICATIONS. (Name of the Publication: Nagao Y, Takada H, Miyadai M, Adachi T, Seki R, Kamei Y, Hara I, Taniguchi Y, Naruse K, Hibi M, Kelsh RN, Hashimoto H. (2018) Distinct interactions of Sox5 and Sox10 in fate specification of pigment cells in medaka and zebrafish. PLoS Genet, 14: e1007260.) ■ The RECIPIENT shall give proper credit to the DEVELOPER in any publications reporting results of research using any derivatives developed from the BIOLOGICAL MATERIALS (hereinafter referred to as “the DERIVATIVES”), and shall procure other institutionsto which the DERIVATIVES are provided (including when provided through the NBRP) to do the same. |
| Category | Transgenic strains |
| Organization | National Institute for Basic Biology |
| Frozen sperm in NIBB | ○ |
| Deposited by | Nagoya University |
| Order |
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| Image |
Transgene map_TgBAC(pax7aloxp-dsred-loxp-sox5-2A-venus) |
| Image |
TG1210_sox5^ml-3ml-3 TgBAC(pax7aloxp-dsred-loxp-sox5^WT-2A-venus) In the absence of cre mRNA, sox5-/-;Tg embryos (sox5-/-;TgBAC(pax7a:loxp-dsred-loxp-sox5^WT)) were strongly expressing Dsred (upper panels, no injection). When cre mRNA was injected in the 1-cell sox5-/-;Tg embryos, they became positive for Venus (lower panels, injection with Cre mRNA). Venus-expressing animals showed rescued xanthophore and leucophore phenotypes in sox5-/- mutants. |
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