11.1.1 PAS staining

FrontPage

11.1.1 Periodic acid-Schiff (PAS) staining of the kidney†

Emiko Mochiduki
Tomohiro Harada
Nagoya University

11.1.1.1 Procedure†

1. Fix the tissues with Bouin?s fixative.
2. Prepare paraffin sections by a conventional method.
3. Put deparaffinized sections in 0.5% periodic acid solution in 5 min.
4. Rinse sections in two changes of distilled water (DW) for 1 min. each.
5. Put sections in cold Schiff?s reagent for 5 min.
6. Put sections in three changes of H2SO3 solution for three min. each.
7. Rinse sections in running tap water for ten min. Regulate the time with checking the degree of staining.
8. Put sections in hematoxylin for 3-4 min.
9. Rinse sections in running tap water for ten min. Regulate the time with checking the degree of staining.
10. Dehydrate and mount sections by a conventional method.

11.1.1.2 Notices†

• All procedures are processed at room temperature.
• Bouin?s fixative:
  Saturated picric acid solution 15 ml
  Formaldehyde solution 5 ml
  Acetic acid 1 ml
  Mix the above solutions before use.
• Cold Schiff:
  Stock a commercially available reagent at 4?, and keep it at room temperature for 30 min. before use. The reagent losing the odor cannot be used.
• H2SO3 solution:
  10%NaHSO3 6 ml
  1N HCl 5 ml
  DW 100 ml
  
• Staining of the kidney:
  A photo of PAS-stained section is attached.
  

  #ref(): File not found: "PAS2.jpg" at page "11.1.1 PAS staining"

  The basement membrane of the renal tubules, glomerulus and Bouman?s capsule, and the apical surface of the proximal tubule segment is stained with purple color. d/c, distal or collecting tubule segment; g, glomerulus; p, proximal tubule segment; l, lymphoid tissues.