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sox5^N541S
| 系統情報 | |
|---|---|
| 系統ID | MT1048 |
| 系統名 | sox5^N541S |
| 系統型 | 突然変異 |
| 特徴 | As a result of TILLING screening, TILLING_MUTANT(ID:1325) (TA1325) was identified as a mutant with a single nucleotide substitution in sox5 exon13. Homozygous mutant embryos and larvae have excess leucophores and lack xanthophores. Similarly, homozygous adult fish have excess leucophores, but unlike at earlier stages, they have xanthophores significantly reduced in number. Formation of melanophores and iridophores is not affected in this mutant. This strain is OK-Cab background. |
成果論文
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| 特記事項 [連絡先] |
■ 利用者は、すべての本件刊行物において、次の文献を引用する。 （文献名：High-resolution melting curve analysis for rapid detection of mutations in a Medaka TILLING library. Ishikawa T, Kamei Y, Otozai S, Kim J, Sato A, Kuwahara Y, Tanaka M, Deguchi T, Inohara H, Tsujimura T, Todo T. BMC Mol Biol. 2010 Sep 15;11:70. TANIGUCHI, Yoshihito, et al. Generation of medaka gene knockout models by target-selected mutagenesis. Genome biology, 2006, 7.12: R116. NAGAO, Yusuke, et al. Sox5 functions as a fate switch in medaka pigment cell development. PLoS Genet, 2014, 10.4: e1004246.) |
| 分類 | ENU突然変異系統 (TILLING) |
| 提供機関 | 基礎生物学研究所 |
| 凍結精子[基生研] | ○ |
| 寄託機関 | 名古屋大学 |
| Order |
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| Image |
MT1048-1049 sox5 mutant The mutant hatchlings exhibit excess and ectopic leucophores and no xanthophore. Leucophores are shown in the darkfield image (left panel). Xanthophores are not detectable under UV light (right panel). |
| Document (PDF) | MT1048_Genotyping protocol |
| Document (PDF) | sox5 cDNA seq |
2 件の成果論文が登録されています
