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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
9539R-3 |
| Symbol |
Sec61alpha |
Full Name |
Sec61alpha |
| CG No |
CG9539 |
Old CG No |
CG9539 |
| Synonyms |
E(br)165, Sec61a, Sec61alpha, Sec61, sec61, endd2, DSec61alpha, LD29847, l(2)k04917, 0455, CG9539, BcDNA:LD29847, BEST:LD29847, anon-WO0118547.277, anon-WO0118547.195 |
| Accession No (Link to NCBI) |
NM_135190.3 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Arsham AM, Neufeld TP.
A genetic screen in Drosophila reveals novel cytoprotective functions of the autophagy-lysosome pathway.
PLoS One (2009) 4(6) e6068
[
PubMed ID = 19562034
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGAGGCCTTCTATCGCCAGAAC |
| Primer Seq. 3' |
aatctagaggtaccTGCTGCTCCTTCAGCTGCTTG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GNNGCCTTCT ATCGCCAGAA CCTGCCCAAT CTGATGAACC TGCTGGCCAC CGTGCTGGTG
0061 TTCGCCGTGG TCATATACTT CCAAGGCTTC CGCGTGGACC TACCCATCAA GAGCGCCCGT
0121 TACCGCGGCC AGTACAGCAG CTATCCCATC AAGCTGTTCT ACACCTCCAA CATTCCCATC
0181 ATCCTGCAGT CTGCGCTCGT GTCCAACTTG TACGTCATCT CCCAGATGCT GGCTGTCAAG
0241 TTCCAGGGCA ACTTCTTCAT TAACCTTCTG GGTGTGTGGG CTGATGTTGG AGGCGGCGGC
0301 CCGGCTCGCT CCTATCCCAT CGGTGGTCTG TGCTACTATC TTTCGCCCCC AGAGAGCGTG
0361 GGCCACATCC TGACCGACCC CATTCACGCG TTGCTCTACA TTGTCTTCAT GTTGGGCTCG
0421 TGCGCCTTCT TCTCCAAGAC CTGGATCGAT GTGTCCGGCA GCTCAGCCAA GGATGTTgNC
0481 AAGCAGCTGA AGGAGCAGCA |
| in silico PCR Fragment |
0001 GAGGCCTTCT ATCGCCAGAA CCTGCCCAAT CTGATGAACC TGCTGGCCAC CGTGCTGGTG
0061 TTCGCCGTGG TCATATACTT CCAAGGCTTC CGCGTGGACC TACCCATCAA GAGCGCCCGT
0121 TACCGCGGCC AGTACAGCAG CTATCCCATC AAGCTGTTCT ACACCTCCAA CATTCCCATC
0181 ATCCTGCAGT CTGCGCTCGT GTCCAACTTG TACGTCATCT CCCAGATGCT GGCTGTCAAG
0241 TTCCAGGGCA ACTTCTTCAT TAACCTTCTG GGTGTGTGGG CTGATGTTGG AGGCGGCGGC
0301 CCGGCTCGCT CCTATCCCAT CGGTGGTCTG TGCTACTATC TTTCGCCCCC AGAGAGCGTG
0361 GGCCACATCC TGACCGACCC CATTCACGCG TTGCTCTACA TTGTCTTCAT GTTGGGCTCG
0421 TGCGCCTTCT TCTCCAAGAC CTGGATCGAT GTGTCCGGCA GCTCAGCCAA GGATGTTGCC
0481 AAGCAGCTGA AGGAGCAGCA |
| Assemble Data |
9539R-3.IR_full 1 GNNGCCTTCTATCGCCAGAACCTGCCCAATCTGATGAACCTGCTGGCCACCGTGCTGGTG 60
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9539R-3.in silico 1 GAGGCCTTCTATCGCCAGAACCTGCCCAATCTGATGAACCTGCTGGCCACCGTGCTGGTG 60
9539R-3.IR_full 61 TTCGCCGTGGTCATATACTTCCAAGGCTTCCGCGTGGACCTACCCATCAAGAGCGCCCGT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9539R-3.in silico 61 TTCGCCGTGGTCATATACTTCCAAGGCTTCCGCGTGGACCTACCCATCAAGAGCGCCCGT 120
9539R-3.IR_full 121 TACCGCGGCCAGTACAGCAGCTATCCCATCAAGCTGTTCTACACCTCCAACATTCCCATC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9539R-3.in silico 121 TACCGCGGCCAGTACAGCAGCTATCCCATCAAGCTGTTCTACACCTCCAACATTCCCATC 180
9539R-3.IR_full 181 ATCCTGCAGTCTGCGCTCGTGTCCAACTTGTACGTCATCTCCCAGATGCTGGCTGTCAAG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9539R-3.in silico 181 ATCCTGCAGTCTGCGCTCGTGTCCAACTTGTACGTCATCTCCCAGATGCTGGCTGTCAAG 240
9539R-3.IR_full 241 TTCCAGGGCAACTTCTTCATTAACCTTCTGGGTGTGTGGGCTGATGTTGGAGGCGGCGGC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9539R-3.in silico 241 TTCCAGGGCAACTTCTTCATTAACCTTCTGGGTGTGTGGGCTGATGTTGGAGGCGGCGGC 300
9539R-3.IR_full 301 CCGGCTCGCTCCTATCCCATCGGTGGTCTGTGCTACTATCTTTCGCCCCCAGAGAGCGTG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9539R-3.in silico 301 CCGGCTCGCTCCTATCCCATCGGTGGTCTGTGCTACTATCTTTCGCCCCCAGAGAGCGTG 360
9539R-3.IR_full 361 GGCCACATCCTGACCGACCCCATTCACGCGTTGCTCTACATTGTCTTCATGTTGGGCTCG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9539R-3.in silico 361 GGCCACATCCTGACCGACCCCATTCACGCGTTGCTCTACATTGTCTTCATGTTGGGCTCG 420
9539R-3.IR_full 421 TGCGCCTTCTTCTCCAAGACCTGGATCGATGTGTCCGGCAGCTCAGCCAAGGATGTTGNC 480
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
9539R-3.in silico 421 TGCGCCTTCTTCTCCAAGACCTGGATCGATGTGTCCGGCAGCTCAGCCAAGGATGTTGCC 480
9539R-3.IR_full 481 AAGCAGCTGAAGGAGCAGCA 500
||||||||||||||||||||
9539R-3.in silico 481 AAGCAGCTGAAGGAGCAGCA 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_135190.3 |
CG9539-RA (Sec61alpha), mRNA |
| 0.2 |
1 |
3 |
3 |
3 |
NM_165193.1 |
CG17932-RB, transcript variant B (Ugt36Bc), mRNA |
| 0.2 |
1 |
3 |
3 |
3 |
NM_144370.2 |
CG17932-RA, transcript variant A (Ugt36Bc), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_001043156.1 |
CG8779-RB, transcript variant B (nrm), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_079494.3 |
CG8779-RA, transcript variant A (nrm), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_176415.1 |
CG1142-RA (CG1142), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_139769.1 |
CG10467-RA (CG10467), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_167271.1 |
CG32667-RA (CG32667), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_137831.2 |
CG4752-RA (CG4752), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_001031964.1 |
CG13702-RC, transcript variant C (AlCR2), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_140782.4 |
CG13702-RB, transcript variant B (AlCR2), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_141798.1 |
CG10703-RA (CG10703), mRNA |
| 0 |
0 |
1 |
3 |
1 |
NM_057751.2 |
CG8161-RA (Rlb1), mRNA |
| 0 |
0 |
1 |
3 |
1 |
NM_130542.2 |
CG11403-RA (CG11403), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_132303.1 |
CG11042-RA (CG11042), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_078540.4 |
CG8989-RA, transcript variant A (His3.3B), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_176719.1 |
CG8989-RC, transcript variant C (His3.3B), mRNA |
| 0 |
0 |
1 |
2 |
8 |
NM_167180.3 |
CG8989-RB, transcript variant B (His3.3B), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_133092.1 |
CG6585-RA (Cyp308a1), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_137136.1 |
CG12866-RA (CG12866), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_139612.1 |
CG1308-RA (CG1308), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_079587.2 |
CG4649-RA (Sodh-2), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001015239.1 |
CG17172-PC.3 (CG17172), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_080017.2 |
CG7528-RA (Uba2), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001014504.1 |
CG30372-RB (CG30372), mRNA |
| 0 |
0 |
1 |
1 |
7 |
NM_142942.3 |
CG31140-RA, transcript variant A (CG31140), mRNA |
| 0 |
0 |
1 |
1 |
6 |
NM_206553.1 |
CG31140-RB, transcript variant B (CG31140), mRNA |
| 0 |
0 |
1 |
1 |
5 |
NM_206552.1 |
CG31140-RC, transcript variant C (CG31140), mRNA |
| 0 |
0 |
1 |
1 |
2 |
NM_170465.1 |
CG31031-RA (CG31031), mRNA |
| 0 |
0 |
1 |
1 |
2 |
NM_136542.1 |
CG30361-RA, transcript variant A (mXr), mRNA |
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