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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
8376R-2 |
| Symbol |
ap |
Full Name |
apterous |
| CG No |
CG8376 |
Old CG No |
CG8376 |
| Synonyms |
S-2a, CG8376, LIM, blt, Xa, trw, ap, Ap |
| Accession No (Link to NCBI) |
NM_165445.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244
[
PubMed ID = 27908785
]
[
RRC reference
]
Eade KT, Fancher HA, Ridyard MS, Allan DW.
Developmental transcriptional networks are required to maintain neuronal subtype identity in the mature nervous system.
PLoS Genet (2012) 8(2) e1002501
[
PubMed ID = 22383890
]
[
RRC reference
]
Natori K, Tajiri R, Furukawa S, Kojima T.
Progressive tarsal patterning in the Drosophila by temporally dynamic regulation of transcription factor genes.
Dev Biol (2012) 361(2) 450-62
[
PubMed ID = 22079694
]
[
RRC reference
]
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATAACGCGCAACCTCGACGAC |
| Primer Seq. 3' |
aatctagaggtaccGGAACAATGCTCCGACTAGGG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ATAACGCGCA ACCTCGACGA CCTGCTCCGG CTGCGGACGT CAGATACAGG ATCGCTTCTA
0061 CCTCTCCGCT GTGGAAAAAC GGTGGCATGC AAGTTGCCTA CAGTGCTACG CCTGTCGGCA
0121 GCCGCTGGAA CGGGAATCCT CATGCTACTC ACGTGACGGC AACATTTATT GCAAAAACGA
0181 TTATTATAGT TTTTTTGGTA CTCGCCGATG CTCGCGCTGC CTGGCCTCCA TCAGCTCCAA
0241 CGAGCTGGTC ATGCGCGCCC GAAATCTTGT TTTTCACGTC AACTGCTTCT GCTGCACTGT
0301 CTGCCACACG CCACTGACAA AGGGAGACCA GTACGGCATC ATCGACGCCC TCATCTACTG
0361 CAGGACCCAC TACAGCATAG CCAGGGAGGG GGATACCGCC TCATCCAGTA TGAGCGCCAC
0421 CTACCCGTAC AGCGCCCAGT TCGGCTCACC CCACAACGAC TCCTCGAGCC CGCACTCGGA
0481 CCCTAGTCGG AGCATTGTTC C |
| in silico PCR Fragment |
0001 ATAACGCGCA ACCTCGACGA -CTGCTCCGG CTGCGGACGT CAGATACAGG ATCGCTTCTA
0061 CCTCTCCGCT GTGGAAAAAC GGTGGCATGC AAGTTGCCTA CAGTGCTACG CCTGTCGGCA
0121 GCCGCTGGAA CGGGAATCCT CATGCTACTC ACGTGACGGC AACATTTATT GCAAAAACGA
0181 TTATTATAGT TTTTTTGGTA CTCGCCGATG CTCGCGCTGC CTGGCCTCCA TCAGCTCCAA
0241 CGAGCTGGTC ATGCGCGCCA GAAATCTTGT TTTTCACGTC AACTGCTTCT GCTGCACTGT
0301 CTGCCACACG CCACTGACAA AGGGAGACCA GTACGGCATC ATCGACGCCC TCATCTACTG
0361 CAGGACCCAC TACAGCATAG CCAGGGAGGG GGATACCGCC TCATCCAGTA TGAGCGCCAC
0421 CTACCCGTAC AGCGCCCAGT TCGGCTCACC CCACAACGAC TCCTCGAGCC CGCACTCGGA
0481 CCCTAGTCGG AGCATTGTTC C |
| Assemble Data |
8376R-2.IR_full 1 ATAACGCGCAACCTCGACGACCTGCTCCGGCTGCGGACGTCAGATACAGGATCGCTTCTA 60
|||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||
8376R-2.in silico 1 ATAACGCGCAACCTCGACGA-CTGCTCCGGCTGCGGACGTCAGATACAGGATCGCTTCTA 60
8376R-2.IR_full 61 CCTCTCCGCTGTGGAAAAACGGTGGCATGCAAGTTGCCTACAGTGCTACGCCTGTCGGCA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8376R-2.in silico 61 CCTCTCCGCTGTGGAAAAACGGTGGCATGCAAGTTGCCTACAGTGCTACGCCTGTCGGCA 120
8376R-2.IR_full 121 GCCGCTGGAACGGGAATCCTCATGCTACTCACGTGACGGCAACATTTATTGCAAAAACGA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8376R-2.in silico 121 GCCGCTGGAACGGGAATCCTCATGCTACTCACGTGACGGCAACATTTATTGCAAAAACGA 180
8376R-2.IR_full 181 TTATTATAGTTTTTTTGGTACTCGCCGATGCTCGCGCTGCCTGGCCTCCATCAGCTCCAA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8376R-2.in silico 181 TTATTATAGTTTTTTTGGTACTCGCCGATGCTCGCGCTGCCTGGCCTCCATCAGCTCCAA 240
8376R-2.IR_full 241 CGAGCTGGTCATGCGCGCCCGAAATCTTGTTTTTCACGTCAACTGCTTCTGCTGCACTGT 300
||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||
8376R-2.in silico 241 CGAGCTGGTCATGCGCGCCAGAAATCTTGTTTTTCACGTCAACTGCTTCTGCTGCACTGT 300
8376R-2.IR_full 301 CTGCCACACGCCACTGACAAAGGGAGACCAGTACGGCATCATCGACGCCCTCATCTACTG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8376R-2.in silico 301 CTGCCACACGCCACTGACAAAGGGAGACCAGTACGGCATCATCGACGCCCTCATCTACTG 360
8376R-2.IR_full 361 CAGGACCCACTACAGCATAGCCAGGGAGGGGGATACCGCCTCATCCAGTATGAGCGCCAC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8376R-2.in silico 361 CAGGACCCACTACAGCATAGCCAGGGAGGGGGATACCGCCTCATCCAGTATGAGCGCCAC 420
8376R-2.IR_full 421 CTACCCGTACAGCGCCCAGTTCGGCTCACCCCACAACGACTCCTCGAGCCCGCACTCGGA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8376R-2.in silico 421 CTACCCGTACAGCGCCCAGTTCGGCTCACCCCACAACGACTCCTCGAGCCCGCACTCGGA 480
8376R-2.IR_full 481 CCCTAGTCGGAGCATTGTTCC 501
|||||||||||||||||||||
8376R-2.in silico 481 CCCTAGTCGGAGCATTGTTCC 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_165445.2 |
CG8376-RA, transcript variant A (ap), mRNA |
| 60.99 |
294 |
1 |
1 |
5 |
NM_078897.3 |
CG8376-RB, transcript variant B (ap), mRNA |
| 0 |
0 |
3 |
6 |
6 |
NM_079178.2 |
CG1921-RB, transcript variant B (sty), mRNA |
| 0 |
0 |
3 |
4 |
9 |
NM_166318.1 |
CG15105-RB, transcript variant B (CG15105), mRNA |
| 0 |
0 |
3 |
4 |
9 |
NM_137546.2 |
CG15105-RA, transcript variant A (CG15105), mRNA |
| 0 |
0 |
3 |
4 |
4 |
NM_168031.1 |
CG1921-RC, transcript variant C (sty), mRNA |
| 0 |
0 |
2 |
5 |
9 |
NM_133012.2 |
CG32560-RA (CG32560), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_167676.1 |
CG12529-RB, transcript variant B (Zw), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_078687.1 |
CG12529-RA, transcript variant A (Zw), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_143101.1 |
CG11854-RA (CG11854), mRNA |
| 0 |
0 |
1 |
9 |
4 |
NM_080073.2 |
CG17697-RA, transcript variant A (fz), mRNA |
| 0 |
0 |
1 |
6 |
3 |
NM_001043297.1 |
CG3339-RB, transcript variant B (CG3339), mRNA |
| 0 |
0 |
1 |
6 |
3 |
NM_143300.1 |
CG3339-RA, transcript variant A (CG3339), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_142931.3 |
CG10192-RA (CG10192), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_168271.1 |
CG7118-RA (Jon66Ci), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_057368.3 |
CG2621-RD, transcript variant D (sgg), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_137931.1 |
CG13541-RA (CG13541), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_167307.1 |
CG4147-RC, transcript variant C (Hsc70-3), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_167306.1 |
CG4147-RA, transcript variant A (Hsc70-3), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_167308.1 |
CG4147-RD, transcript variant D (Hsc70-3), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_078577.2 |
CG4147-RB, transcript variant B (Hsc70-3), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_135755.1 |
CG9932-RA (CG9932), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_134507.1 |
CG14234-RA (CG14234), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_079749.4 |
CG5405-RA, transcript variant A (KrT95D), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_170108.2 |
CG5405-RB, transcript variant B (KrT95D), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_170110.2 |
CG5405-RC, transcript variant C (KrT95D), mRNA |
| 0 |
0 |
0 |
8 |
5 |
NM_137402.2 |
CG4966-RA, transcript variant A (CG4966), mRNA |
| 0 |
0 |
0 |
6 |
29 |
NM_001015110.1 |
CG40323-PB.3 (CG40323), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_141408.3 |
CG1137-RA (CG1137), mRNA |
| 0 |
0 |
0 |
5 |
8 |
NM_142646.1 |
CG5483-RA (CG5483), mRNA |
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