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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
8222R-3 |
| Symbol |
Pvr |
Full Name |
PDGF- and VEGF-receptor related |
| CG No |
CG8222 |
Old CG No |
CG8222 |
| Synonyms |
VEGFR, PVR, pvr, CG8222, VEGFR-A, Vegfr-c, Vegfr-b, stai, Vegfr, DmVEGFR, vgr1, VGR1, CT24332, Vgr1, Pvr |
| Accession No (Link to NCBI) |
NM_078785.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
semi-lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tsai CR, Wang Y, Jacobson A, Sankoorikkal N, Chirinos JD, Burra S, Makthal N, Kumaraswami M, Galko MJ.
Pvr and distinct downstream signaling factors are required for hemocyte spreading and epidermal wound closure at Drosophila larval wound sites.
G3 (Bethesda) (2022) 12(1)
[
PubMed ID = 34751396
]
[
RRC reference
]
Ohhara Y, Hoshino G, Imahori K, Matsuyuki T, Yamakawa-Kobayashi K.
The Nutrient-Responsive Molecular Chaperone Hsp90 Supports Growth and Development in Drosophila.
Front Physiol (2021) 12 690564
[
PubMed ID = 34239451
]
[
RRC reference
]
Lopez-Bellido R, Puig S, Huang PJ, Tsai CR, Turner HN, Galko MJ, Gutstein HB.
Growth Factor Signaling Regulates Mechanical Nociception in Flies and Vertebrates.
J Neurosci (2019) 39(30) 6012-6030
[
PubMed ID = 31138657
]
[
RRC reference
]
Sansone CL, Cohen J, Yasunaga A, Xu J, Osborn G, Subramanian H, Gold B, Buchon N, Cherry S.
Microbiota-Dependent Priming of Antiviral Intestinal Immunity in Drosophila.
Cell Host Microbe (2015) 18(5) 571-81
[
PubMed ID = 26567510
]
[
RRC reference
]
Garlena RA, Lennox AL, Baker LR, Parsons TE, Weinberg SM, Stronach BE.
The receptor tyrosine kinase Pvr promotes tissue closure by coordinating corpse removal and epidermal zippering.
Development (2015) 142(19) 3403-15
[
PubMed ID = 26293306
]
[
RRC reference
]
Tsuzuki S, Matsumoto H, Furihata S, Ryuda M, Tanaka H, Sung EJ, Bird GS, Zhou Y, Shears SB, Hayakawa Y.
Switching between humoral and cellular immune responses in Drosophila is guided by the cytokine GBP.
Nat Commun (2014) 5 4628
[
PubMed ID = 25130174
]
[
RRC reference
]
Tran TA, Kinch L, Peña-Llopis S, Kockel L, Grishin N, Jiang H, Brugarolas J.
Platelet-derived growth factor/vascular endothelial growth factor receptor inactivation by sunitinib results in Tsc1/Tsc2-dependent inhibition of TORC1.
Mol Cell Biol (2013) 33(19) 3762-79
[
PubMed ID = 23878397
]
[
RRC reference
]
Wu Y, Brock AR, Wang Y, Fujitani K, Ueda R, Galko MJ.
A blood-borne PDGF/VEGF-like ligand initiates wound-induced epidermal cell migration in Drosophila larvae.
Curr Biol (2009) 19(17) 1473-7
[
PubMed ID = 19646875
]
[
RRC reference
]
Ishimaru S, Ueda R, Hinohara Y, Ohtani M, Hanafusa H.
PVR plays a critical role via JNK activation in thorax closure during Drosophila metamorphosis.
EMBO J (2004) 23(20) 3984-94
[
PubMed ID = 15457211
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGCTTCCGCGGTTGATTCTGC |
| Primer Seq. 3' |
aatctagaggtaccTGCCGTCACCACGTTATGACT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TGCTTCCGCG GTTGATTCTG CTGCCACTGC TCCTGATTTT GCGGATCTCG TGGAGCGATG
0061 CTGTgNCTTT GCAGCAGTTC TCACCGGATC CCGATGACAG CATCGAGAAC TGCGGCGGCG
0121 AAAATGGAGC TCCCCTGATG ACGCCCTGCA AGAGCGCCAT TATCCCTCGA TGCCCAGACG
0181 AGCACCCACG CTAAAGTGCG AGGACGACGA GCCGATGAGC TGGTGGACCA GTCAATCGCA
0241 ATATGTGCAT GTAAAGTCCT TCGATAATAC GGAGGATCCG GCTCGACCAT TCGGAACTAG
0301 CCTGCATCTC ATCGAAGTGA CGGCTGACTA TGTGGCAGCC TACTATTGCG TGAAGACTTC
0361 GAAATTCAGT CAGATCGCCA AGGAGGAGCA GTCGGACGAG GCGATGATCG AATTGGTTAA
0421 TCAAGGATAC GCCAGCTCCA TCTACGTGTA CGTGAATGAT CCGGACACTA AGCTGGTCGA
0481 TAGTCATAAC GTGGTGACGG CA |
| in silico PCR Fragment |
0001 TGCTTCCGCG GTTGATTCTG CTGCCACTGC TCCTGATTTT GCGGATCTCG TGGAGCGATG
0061 CTGTGCCTTT GCAGCAGTTC TCACCGGATC CCGATGACAG CATCGAGAAC TGCGGCGGCG
0121 AGAATGGAGC TCCCCTGATG ACGCCCTGCA AGAGCGCCAT TAT-CCTGGA TGCCCAGACG
0181 AGCA-CCACG CTTAAGTGCG AGGACGACGA GCCGATGAGC TGGTGGACCA GTCAATCGCA
0241 ATATGTGCAT GTGAAGTCCT TCGATAATAC GGAGGATCCG GCTCGACCAT TCGGAACTAG
0301 CCTGCATCTC ATCGAAGTGA CGGCTGACTA TGTGGCAGCC TACTATTGCG TGAAGACTTC
0361 GAAATTCAGT CAGATCGCCA AGGAGGAGCA GTCGGACGAG GCGATGATCG AATTGGTTAA
0421 TCAAGGATAC GCCAGCTCCA TCTACGTGTA CGTGAATGAT CCGGACACTA AGCTGGTCGA
0481 TAGTCATAAC GTGGTGACGG CA |
| Assemble Data |
8222R-3.IR_full 1 TGCTTCCGCGGTTGATTCTGCTGCCACTGCTCCTGATTTTGCGGATCTCGTGGAGCGATG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8222R-3.in silico 1 TGCTTCCGCGGTTGATTCTGCTGCCACTGCTCCTGATTTTGCGGATCTCGTGGAGCGATG 60
8222R-3.IR_full 61 CTGTGNCTTTGCAGCAGTTCTCACCGGATCCCGATGACAGCATCGAGAACTGCGGCGGCG 120
||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
8222R-3.in silico 61 CTGTGCCTTTGCAGCAGTTCTCACCGGATCCCGATGACAGCATCGAGAACTGCGGCGGCG 120
8222R-3.IR_full 121 AAAATGGAGCTCCCCTGATGACGCCCTGCAAGAGCGCCATTATCCCTCGATGCCCAGACG 180
| ||||||||||||||||||||||||||||||||||||||||| ||| ||||||||||||
8222R-3.in silico 121 AGAATGGAGCTCCCCTGATGACGCCCTGCAAGAGCGCCATTAT-CCTGGATGCCCAGACG 180
8222R-3.IR_full 181 AGCACCCACGCTAAAGTGCGAGGACGACGAGCCGATGAGCTGGTGGACCAGTCAATCGCA 240
|||| ||||||| |||||||||||||||||||||||||||||||||||||||||||||||
8222R-3.in silico 181 AGCA-CCACGCTTAAGTGCGAGGACGACGAGCCGATGAGCTGGTGGACCAGTCAATCGCA 240
8222R-3.IR_full 241 ATATGTGCATGTAAAGTCCTTCGATAATACGGAGGATCCGGCTCGACCATTCGGAACTAG 300
|||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
8222R-3.in silico 241 ATATGTGCATGTGAAGTCCTTCGATAATACGGAGGATCCGGCTCGACCATTCGGAACTAG 300
8222R-3.IR_full 301 CCTGCATCTCATCGAAGTGACGGCTGACTATGTGGCAGCCTACTATTGCGTGAAGACTTC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8222R-3.in silico 301 CCTGCATCTCATCGAAGTGACGGCTGACTATGTGGCAGCCTACTATTGCGTGAAGACTTC 360
8222R-3.IR_full 361 GAAATTCAGTCAGATCGCCAAGGAGGAGCAGTCGGACGAGGCGATGATCGAATTGGTTAA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8222R-3.in silico 361 GAAATTCAGTCAGATCGCCAAGGAGGAGCAGTCGGACGAGGCGATGATCGAATTGGTTAA 420
8222R-3.IR_full 421 TCAAGGATACGCCAGCTCCATCTACGTGTACGTGAATGATCCGGACACTAAGCTGGTCGA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8222R-3.in silico 421 TCAAGGATACGCCAGCTCCATCTACGTGTACGTGAATGATCCGGACACTAAGCTGGTCGA 480
8222R-3.IR_full 481 TAGTCATAACGTGGTGACGGCA 502
||||||||||||||||||||||
8222R-3.in silico 481 TAGTCATAACGTGGTGACGGCA 502
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_205927.1 |
CG8222-RD, transcript variant D (Pvr), mRNA |
| 100 |
482 |
0 |
0 |
2 |
NM_164801.1 |
CG8222-RB, transcript variant B (Pvr), mRNA |
| 100 |
482 |
0 |
0 |
2 |
NM_078785.2 |
CG8222-RA, transcript variant A (Pvr), mRNA |
| 100 |
482 |
0 |
0 |
2 |
NM_205925.1 |
CG8222-RF, transcript variant F (Pvr), mRNA |
| 100 |
482 |
0 |
0 |
2 |
NM_205926.1 |
CG8222-RE, transcript variant E (Pvr), mRNA |
| 86.92 |
419 |
1 |
1 |
3 |
NM_175983.1 |
CG8222-RC, transcript variant C (Pvr), mRNA |
| 0 |
0 |
4 |
3 |
3 |
NM_057631.4 |
CG5786-RA (ppan), mRNA |
| 0 |
0 |
3 |
3 |
6 |
NM_001043216.1 |
CG34113-RO, transcript variant O (CG34113), mRNA |
| 0 |
0 |
3 |
3 |
5 |
NM_001043217.1 |
CG34113-RP, transcript variant P (CG34113), mRNA |
| 0 |
0 |
3 |
2 |
6 |
NM_142463.2 |
CG7678-RA (CG7678), mRNA |
| 0 |
0 |
2 |
4 |
5 |
NM_140132.3 |
CG32056-RA, transcript variant A (CG32056), mRNA |
| 0 |
0 |
2 |
4 |
5 |
NM_168404.1 |
CG32056-RB, transcript variant B (CG32056), mRNA |
| 0 |
0 |
2 |
4 |
5 |
NM_168403.1 |
CG32056-RC, transcript variant C (CG32056), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_132792.2 |
CG6227-RA (CG6227), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_079833.2 |
CG7917-RA (Nlp), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_141800.1 |
CG14701-RA (CG14701), mRNA |
| 0 |
0 |
1 |
4 |
9 |
NM_166096.1 |
CG30470-RA (CG30470), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_169482.1 |
CG7340-RA, transcript variant A (granny-smith), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_169483.1 |
CG7340-RC, transcript variant C (granny-smith), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_141983.2 |
CG7340-RB, transcript variant B (granny-smith), mRNA |
| 0 |
0 |
1 |
3 |
12 |
NM_170367.1 |
CG1866-RA, transcript variant A (Moca-cyp), mRNA |
| 0 |
0 |
1 |
3 |
12 |
NM_143361.3 |
CG1866-RB, transcript variant B (Moca-cyp), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_080157.2 |
CG11648-RB, transcript variant B (Abd-B), mRNA |
| 0 |
0 |
1 |
2 |
10 |
NM_165794.2 |
CG11895-RA (stan), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_168038.1 |
CG32264-RD, transcript variant D (CG32264), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_132164.1 |
CG18155-RA, transcript variant A (CG18155), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_166949.1 |
CG2694-RA, transcript variant A (CG2694), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_130668.2 |
CG2694-RB, transcript variant B (CG2694), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_206637.1 |
CG18155-RB, transcript variant B (CG18155), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_135391.2 |
CG13095-RA (CG13095), mRNA |
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