|
Notice:
|
|
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
7833R-3 |
| Symbol |
Orc5 |
Full Name |
Origin recognition complex subunit 5 |
| CG No |
CG7833 |
Old CG No |
CG7833 |
| Synonyms |
ORC5, ORC, l(2)34Df, orc5, BG:DS00941.8, DmORC5, l(2)br39, br39, CG7833, Orc5, Origin recognition complex subunit 5, lethal(2)34Df |
| Accession No (Link to NCBI) |
NM_057784.4 |
| Inserted Chr. |
ll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Herr A, Longworth M, Ji JY, Korenjak M, Macalpine DM, Dyson NJ.
Identification of E2F target genes that are rate limiting for dE2F1-dependent cell proliferation.
Dev Dyn (2012) 241(11) 1695-707
[
PubMed ID = 22972499
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATTGAAACGCTGGGCGAGCTG |
| Primer Seq. 3' |
aatctagaggtaccTTGTTGTACTGCGCCAAGTGC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ATTGAAACGC TGGGCGAGCT GATAGGAGAT TCGAGTGAGA CATATCCCTC GGCAATTTAC
0061 CTATTCGGAC ACAGTGGAAC CGGCAAAACA GCTCTGACCA GAGCTTTCCT CAAAGAATGT
0121 GGAAAACGGC AAAATGTCCG CACAGCGCAT TTAAATGCCA TCGAATGCTA CACCACCAAG
0181 ATAATGCTGG AGATCCTGTT AGACTCGCTG GCACCCGATC AAGGTGATGC GCTGAAGGTC
0241 GACAACATGC TGGACTTTGT GGAGCAGCTG CGTCGGCAGG CCGCTACACG CGTGGAGGAT
0301 CAGGGATTTC TCATAGCCGT GGATAACGCG GAAAGGCTGC GCGACATGGA TGCCAATGTG
0361 TTGCCCGTTC TTCTCCGACT GCAGGAGCTG ACCAACCTGA ATCTGTGCGT TATTCTGCTC
0421 TCCCAGCTGC CCTTTGAGAA GTTCTACAAC AAGACCGGAC TTAGTGAAAT CGTCTGCCTG
0481 CACTTGGCGC AGTACAACAA |
| in silico PCR Fragment |
0001 ATTGAAACGC TGGGCGAGCT GATAGGAGAT TCGAGTGAGA CATATCCCTC GGCAATTTAC
0061 CTATTCGGAC ACAGTGGAAC CGGCAAAACA GCTCTGACCA GAGCTTTCCT CAAAGAATGT
0121 GGAAAACGGC AAAATGTCCG CACAGCGCAT TTAAATGCCA TCGAATGCTA CACCACCAAG
0181 ATAATGCTGG AGATCCTGTT AGACTCGCTG GCACCCGATC AAGGTGATGC GCTGAAGGTC
0241 GACAACATGC TGGACTTTGT GGAGCAGCTG CGTCGGCAGG CCGCTACACG CGTGGAGGAT
0301 CAGGGATTTC TCATAGCCGT GGATAACGCG GAAAGGCTGC GCGACATGGA TGCCAATGTG
0361 TTGCCCGTTC TTCTCCGACT GCAGGAGCTG ACCAACCTGA ATCTGTGCGT TATTCTGCTC
0421 TCCCAGCTGC CCTTTGAGAA GTTCTACAAC AAGACCGGAC TTAGTGAAAT CGTCTGCCTG
0481 CACTTGGCGC AGTACAACAA |
| Assemble Data |
7833R-3.IR_full 1 ATTGAAACGCTGGGCGAGCTGATAGGAGATTCGAGTGAGACATATCCCTCGGCAATTTAC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7833R-3.in silico 1 ATTGAAACGCTGGGCGAGCTGATAGGAGATTCGAGTGAGACATATCCCTCGGCAATTTAC 60
7833R-3.IR_full 61 CTATTCGGACACAGTGGAACCGGCAAAACAGCTCTGACCAGAGCTTTCCTCAAAGAATGT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7833R-3.in silico 61 CTATTCGGACACAGTGGAACCGGCAAAACAGCTCTGACCAGAGCTTTCCTCAAAGAATGT 120
7833R-3.IR_full 121 GGAAAACGGCAAAATGTCCGCACAGCGCATTTAAATGCCATCGAATGCTACACCACCAAG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7833R-3.in silico 121 GGAAAACGGCAAAATGTCCGCACAGCGCATTTAAATGCCATCGAATGCTACACCACCAAG 180
7833R-3.IR_full 181 ATAATGCTGGAGATCCTGTTAGACTCGCTGGCACCCGATCAAGGTGATGCGCTGAAGGTC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7833R-3.in silico 181 ATAATGCTGGAGATCCTGTTAGACTCGCTGGCACCCGATCAAGGTGATGCGCTGAAGGTC 240
7833R-3.IR_full 241 GACAACATGCTGGACTTTGTGGAGCAGCTGCGTCGGCAGGCCGCTACACGCGTGGAGGAT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7833R-3.in silico 241 GACAACATGCTGGACTTTGTGGAGCAGCTGCGTCGGCAGGCCGCTACACGCGTGGAGGAT 300
7833R-3.IR_full 301 CAGGGATTTCTCATAGCCGTGGATAACGCGGAAAGGCTGCGCGACATGGATGCCAATGTG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7833R-3.in silico 301 CAGGGATTTCTCATAGCCGTGGATAACGCGGAAAGGCTGCGCGACATGGATGCCAATGTG 360
7833R-3.IR_full 361 TTGCCCGTTCTTCTCCGACTGCAGGAGCTGACCAACCTGAATCTGTGCGTTATTCTGCTC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7833R-3.in silico 361 TTGCCCGTTCTTCTCCGACTGCAGGAGCTGACCAACCTGAATCTGTGCGTTATTCTGCTC 420
7833R-3.IR_full 421 TCCCAGCTGCCCTTTGAGAAGTTCTACAACAAGACCGGACTTAGTGAAATCGTCTGCCTG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7833R-3.in silico 421 TCCCAGCTGCCCTTTGAGAAGTTCTACAACAAGACCGGACTTAGTGAAATCGTCTGCCTG 480
7833R-3.IR_full 481 CACTTGGCGCAGTACAACAA 500
||||||||||||||||||||
7833R-3.in silico 481 CACTTGGCGCAGTACAACAA 500
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_057784.4 |
CG7833-RA (Orc5), mRNA |
| 0.2 |
1 |
5 |
5 |
2 |
NM_168475.1 |
CG11621-RC, transcript variant C (Pi3K68D), mRNA |
| 0.2 |
1 |
5 |
5 |
2 |
NM_079304.2 |
CG11621-RA, transcript variant A (Pi3K68D), mRNA |
| 0.2 |
1 |
3 |
4 |
2 |
NM_140079.1 |
CG3280-RA (CG3280), mRNA |
| 0.2 |
1 |
2 |
2 |
2 |
NM_132940.1 |
CG13005-RA (CG13005), mRNA |
| 0 |
0 |
6 |
2 |
4 |
NM_170546.1 |
CG31005-RA (CG31005), mRNA |
| 0 |
0 |
4 |
3 |
2 |
NM_080152.2 |
CG5092-RA (Tor), mRNA |
| 0 |
0 |
3 |
4 |
9 |
NM_079706.2 |
CG3412-RA (slmb), mRNA |
| 0 |
0 |
3 |
4 |
4 |
NM_165680.1 |
CG1814-RC, transcript variant C (CG1814), mRNA |
| 0 |
0 |
2 |
4 |
6 |
NM_135235.2 |
CG10805-RA (CG10805), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_169706.1 |
CG4006-RA, transcript variant A (Akt1), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_169707.1 |
CG4006-RB, transcript variant B (Akt1), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_169705.1 |
CG4006-RC, transcript variant C (Akt1), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_142359.2 |
CG31256-RA (Brf), mRNA |
| 0 |
0 |
1 |
8 |
5 |
NM_078938.2 |
CG8261-RC, transcript variant C (Ggamma1), mRNA |
| 0 |
0 |
1 |
8 |
5 |
NM_165633.1 |
CG8261-RD, transcript variant D (Ggamma1), mRNA |
| 0 |
0 |
1 |
8 |
5 |
NM_165632.1 |
CG8261-RB, transcript variant B (Ggamma1), mRNA |
| 0 |
0 |
1 |
8 |
5 |
NM_165631.1 |
CG8261-RA, transcript variant A (Ggamma1), mRNA |
| 0 |
0 |
1 |
8 |
5 |
NM_165634.1 |
CG8261-RE, transcript variant E (Ggamma1), mRNA |
| 0 |
0 |
1 |
7 |
3 |
NM_165238.2 |
CG31741-RA (CG31741), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_165701.1 |
CG1623-RE, transcript variant E (CG1623), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_136676.2 |
CG1623-RC, transcript variant C (CG1623), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_165698.1 |
CG1623-RA, transcript variant A (CG1623), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_165700.1 |
CG1623-RD, transcript variant D (CG1623), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_165699.1 |
CG1623-RB, transcript variant B (CG1623), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_176177.1 |
CG33139-RA (Ranbp11), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_078547.2 |
CG2979-RA (Yp2), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_142685.2 |
CG7034-RA (sec15), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_080014.2 |
CG3064-RB (futsch), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_142586.1 |
CG4538-RA, transcript variant A (CG4538), mRNA |
|
|
|
