NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 6946R-1

Symbol glo Full Name glorund

CG No CG6946 Old CG No CG6946

Synonyms CG6946, l(3)S11046, cg6946, l(3)S011046, 110/46, anon-WO0118547.328, glo, Glo

Accession No (Link to NCBI) NM_141863.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Olesnicky EC, Killian DJ, Garcia E, Morton MC, Rathjen AR, Sola IE, Gavis ER.
Extensive use of RNA-binding proteins in Drosophila sensory neuron dendrite morphogenesis.
G3 (Bethesda) (2014) 4(2) 297-306 [ PubMed ID = 24347626 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAATGGCGACAACTTCAACGAC

Primer Seq. 3'

aatctagaggtaccACAAAAAGTATGCCCTCCCGA

Predicted Fragment Size

IR fragment full Seq

0001 AATGNCGACA ACTTCAACGA CGACAGCAAC CAGCAGCAGG ATGAGGATGA CCAGTACAAC 
0061 GAAGATGGCG GCGGCAAGAT CGAAAACGTC GGCGAGAGCC CCAAATTCGT TCGGTTGCGC 
0121 GGCCTGCCCT GGTCCGCAAC GCACAAGGAG ATACTCGACT TTCTGGAAAA CGTGAATGTG 
0181 ACTAATGGCT CGGCGGGCAT TCACTTGGTC ACCAGTCGGG TCGACGGCAA GAACACCGGC 
0241 GAGGCGTACG TGGAGGTGGC CAGCCAGGAG GACGTCGAAG AGGCTCGCAA GCTGAACAAG 
0301 GCCAGCATGG GTCACCGCTA CATTGAAGTT TTTACCGCCA CGCCCAAGGA GGCCAAGGAA 
0361 GCCATGCGGA AGATCAGCGG ACACGGCACG GCCTTCGTTG TGAAGCTTCG TGGTCTGCCA 
0421 TATGCCGTCA CTGAGCAGCA AATCGAGGAG TTCTTCTCTG GGTTGGATAT CAAAACGGAT 
0481 CGGGAGGGCA TACTTTTTGT

in silico PCR Fragment

0001 AATGGCGACA ACTTCAACGA CGACAGCAAC CAGCAGCAGG ATGAGGATGA CCAGTACAAC 
0061 GAAGATGGCG GCGGCAAGAT CGAAAACGTC GGCGAGAGCC CCAAATTCGT TCGGTTGCGC 
0121 GGCCTGCCCT GGTCCGCAAC GCACAAGGAG ATACTCGACT TTCTGGAAAA CGTGAATGTG 
0181 ACTAATGGCT CGGCGGGCAT TCACCTGGTC ACCAGTCGGG TCGACGGCAA GAACACCGGC 
0241 GAGGCGTACG TGGAGGTGGC CAGCCAGGAG GACGTCGAAG AGGCTCGCAA GCTGAACAAG 
0301 GCCAGCATGG GTCACCGCTA CATTGAAGTT TTTACCGCCA CGCCCAAGGA GGCCAAGGAA 
0361 GCCATGCGGA AGATCAGCGG ACACGGCACG GCCTTCGTTG TGAAGCTTCG TGGTCTGCCA 
0421 TATGCCGTCA CTGAGCAGCA AATCGAGGAG TTCTTCTCTG GGTTGGATAT CAAAACGGAT 
0481 CGGGAGGGCA TACTTTTTGT

Assemble Data


6946R-1.IR_full       1   AATGNCGACAACTTCAACGACGACAGCAACCAGCAGCAGGATGAGGATGACCAGTACAAC 60
                          |||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||
6946R-1.in silico     1   AATGGCGACAACTTCAACGACGACAGCAACCAGCAGCAGGATGAGGATGACCAGTACAAC 60


6946R-1.IR_full       61  GAAGATGGCGGCGGCAAGATCGAAAACGTCGGCGAGAGCCCCAAATTCGTTCGGTTGCGC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6946R-1.in silico     61  GAAGATGGCGGCGGCAAGATCGAAAACGTCGGCGAGAGCCCCAAATTCGTTCGGTTGCGC 120


6946R-1.IR_full       121 GGCCTGCCCTGGTCCGCAACGCACAAGGAGATACTCGACTTTCTGGAAAACGTGAATGTG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6946R-1.in silico     121 GGCCTGCCCTGGTCCGCAACGCACAAGGAGATACTCGACTTTCTGGAAAACGTGAATGTG 180


6946R-1.IR_full       181 ACTAATGGCTCGGCGGGCATTCACTTGGTCACCAGTCGGGTCGACGGCAAGAACACCGGC 240
                          |||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||
6946R-1.in silico     181 ACTAATGGCTCGGCGGGCATTCACCTGGTCACCAGTCGGGTCGACGGCAAGAACACCGGC 240


6946R-1.IR_full       241 GAGGCGTACGTGGAGGTGGCCAGCCAGGAGGACGTCGAAGAGGCTCGCAAGCTGAACAAG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6946R-1.in silico     241 GAGGCGTACGTGGAGGTGGCCAGCCAGGAGGACGTCGAAGAGGCTCGCAAGCTGAACAAG 300


6946R-1.IR_full       301 GCCAGCATGGGTCACCGCTACATTGAAGTTTTTACCGCCACGCCCAAGGAGGCCAAGGAA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6946R-1.in silico     301 GCCAGCATGGGTCACCGCTACATTGAAGTTTTTACCGCCACGCCCAAGGAGGCCAAGGAA 360


6946R-1.IR_full       361 GCCATGCGGAAGATCAGCGGACACGGCACGGCCTTCGTTGTGAAGCTTCGTGGTCTGCCA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6946R-1.in silico     361 GCCATGCGGAAGATCAGCGGACACGGCACGGCCTTCGTTGTGAAGCTTCGTGGTCTGCCA 420


6946R-1.IR_full       421 TATGCCGTCACTGAGCAGCAAATCGAGGAGTTCTTCTCTGGGTTGGATATCAAAACGGAT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6946R-1.in silico     421 TATGCCGTCACTGAGCAGCAAATCGAGGAGTTCTTCTCTGGGTTGGATATCAAAACGGAT 480


6946R-1.IR_full       481 CGGGAGGGCATACTTTTTGT 500
                          ||||||||||||||||||||
6946R-1.in silico     481 CGGGAGGGCATACTTTTTGT 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	4	NM_141863.1	CG6946-RA, transcript variant A (glo), mRNA
100	482	0	0	4	NM_169434.1	CG6946-RB, transcript variant B (glo), mRNA
91.9	443	1	2	5	NM_169435.1	CG6946-RC, transcript variant C (glo), mRNA
0	0	4	5	3	NM_168172.1	CG8368-RB, transcript variant B (CG8368), mRNA
0	0	4	5	3	NM_139793.1	CG8368-RA, transcript variant A (CG8368), mRNA
0	0	3	7	17	NM_080110.2	CG32356-RA, transcript variant A (ImpE1), mRNA
0	0	3	5	8	NM_131954.1	CG7024-RA (CG7024), mRNA
0	0	3	3	9	NM_141529.2	CG11694-RA (CG11694), mRNA
0	0	3	3	3	NM_078807.1	CG4897-RA (RpL7), mRNA
0	0	2	5	8	NM_143744.2	CG5553-RA (DNAprim), mRNA
0	0	2	3	4	NM_135954.1	CG13260-RA (CG13260), mRNA
0	0	1	4	4	NM_057243.3	CG3228-RA (kz), mRNA
0	0	1	4	4	NM_169850.1	CG5558-RB, transcript variant B (CG5558), mRNA
0	0	1	4	3	NM_137585.2	CG11007-RA (CG11007), mRNA
0	0	1	3	12	NM_136942.2	CG8545-RA (CG8545), mRNA
0	0	1	3	11	NM_057951.2	CG9210-RA, transcript variant A (Ac13E), mRNA
0	0	1	3	11	NM_001014745.1	CG9210-RB, transcript variant B (Ac13E), mRNA
0	0	1	3	9	NM_167628.1	CG6816-RA, transcript variant A (Cyp18a1), mRNA
0	0	1	3	9	NM_078679.2	CG6816-RB, transcript variant B (Cyp18a1), mRNA
0	0	1	3	8	NM_170495.1	CG9728-RA (PH4alphaNE1), mRNA
0	0	1	3	6	NM_132932.2	CG9634-RA (CG9634), mRNA
0	0	1	3	5	NM_142181.2	CG4299-RA (Set), mRNA
0	0	1	3	5	NM_139448.2	CG32300-RB (oxt), mRNA
0	0	1	3	5	NM_132817.1	CG9215-RA (CG9215), mRNA
0	0	1	3	4	NM_139457.1	CG13801-RA (CG13801), mRNA
0	0	1	3	3	NM_133103.2	CG7280-RA (CG7280), mRNA
0	0	1	3	3	NM_137629.1	CG13871-RA (CG13871), mRNA
0	0	1	3	3	NM_057324.3	CG5109-RA (Pcl), mRNA
0	0	1	3	3	NM_176273.1	CG33166-RA, transcript variant A (stet), mRNA
0	0	1	3	3	NM_176272.1	CG33166-RB, transcript variant B (stet), mRNA

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