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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
5887R-1 |
| Symbol |
desat1 |
Full Name |
desat1 |
| CG No |
CG5887 |
Old CG No |
CG5887 |
| Synonyms |
Fad, CG5887, fad, desat, l(3)S028813, unnamed, BEST:SD05462, anon-WO0118547.730, anon-WO0118547.726, desat1, Desat1 |
| Accession No (Link to NCBI) |
NM_144474.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Wicker-Thomas C, Guenachi I, Keita YF.
Contribution of oenocytes and pheromones to courtship behaviour in Drosophila.
BMC Biochem (2009) 10 21
[
PubMed ID = 19671131
]
[
RRC reference
]
Chew SK, Chen P, Link N, Galindo KA, Pogue K, Abrams JM.
Genome-wide silencing in Drosophila captures conserved apoptotic effectors.
Nature (2009) 460(7251) 123-7
[
PubMed ID = 19483676
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGACTCCACCGGAGTGCTCTTC |
| Primer Seq. 3' |
aatctagaggtaccAGATCGACTCCCTTGCCCTTG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GACTCCACCG GAGTGCTCTT CGAGTGCGAT GTGGAAACCA CCGACGGTGG TCTTGTCAAG
0061 GACATCACCG TGATGAAGAA GGCCGAGAAG CGCCGCCTGA AGCTCGTCTG GCGCAACATC
0121 ATCGCCTTCG GTTACCTCCA TCTGGCTGCC CTCTACGGAG CCTACCTCAT GGTCACCTCT
0181 GCCAAGTGGC AGACGTGCAT CTTAGCTTAT TTTCTATACG TCATTTCTGG CCTAGGCATT
0241 ACCGCCGGTG CCCATCGTCT GTGGGCGCAT CGCTCCTACA AGGCCAAGTG GCCCCTGCGA
0301 GTGATTCTGG TCATCTTCAA CACGATTGCC TTCCAGGATG CCGCCTACCA TTGGGCTCGC
0361 GACCATCGCG TCCACCACAA ATACTCGGAG ACTGACGCCG ATCCCCACAA CGCCACGCGT
0421 GGTTTCTTCT TCTCGCACGT CGGCTGGCTG CTGTGCAAGA AGCACCCGGA GGTGAAAGCC
0481 AAGGGCAAGG GAGTCGATCT |
| in silico PCR Fragment |
0001 GACTCCACCG GAGTGCTCTT CGAGTGCGAT GTGGAAACCA CCGACGGTGG TCTTGTCAAG
0061 GACATCACCG TGATGAAGAA GGCCGAGAAG CGCCGCCTGA AGCTCGTCTG GCGCAACATC
0121 ATCGCCTTCG GTTACCTCCA TCTGGCTGCC CTCTACGGAG CCTACCTCAT GGTCACCTCT
0181 GCCAAGTGGC AGACGTGCAT CTTAGCTTAT TTTCTATACG TCATTTCTGG CCTAGGCATT
0241 ACCGCCGGTG CCCATCGTCT GTGGGCGCAT CGCTCCTACA AGGCCAAGTG GCCCCTGCGA
0301 GTGATTCTGG TCATCTTCAA CACGATTGCC TTCCAGGATG CCGCCTACCA TTGGGCTCGC
0361 GACCATCGCG TCCACCACAA ATACTCGGAG ACTGACGCCG ATCCCCACAA CGCCACGCGT
0421 GGTTTCTTCT TCTCGCACGT CGGCTGGCTG CTGTGCAAGA AGCACCCGGA GGTGAAAGCC
0481 AAGGGCAAGG GAGTCGATCT |
| Assemble Data |
5887R-1.IR_full 1 GACTCCACCGGAGTGCTCTTCGAGTGCGATGTGGAAACCACCGACGGTGGTCTTGTCAAG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5887R-1.in silico 1 GACTCCACCGGAGTGCTCTTCGAGTGCGATGTGGAAACCACCGACGGTGGTCTTGTCAAG 60
5887R-1.IR_full 61 GACATCACCGTGATGAAGAAGGCCGAGAAGCGCCGCCTGAAGCTCGTCTGGCGCAACATC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5887R-1.in silico 61 GACATCACCGTGATGAAGAAGGCCGAGAAGCGCCGCCTGAAGCTCGTCTGGCGCAACATC 120
5887R-1.IR_full 121 ATCGCCTTCGGTTACCTCCATCTGGCTGCCCTCTACGGAGCCTACCTCATGGTCACCTCT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5887R-1.in silico 121 ATCGCCTTCGGTTACCTCCATCTGGCTGCCCTCTACGGAGCCTACCTCATGGTCACCTCT 180
5887R-1.IR_full 181 GCCAAGTGGCAGACGTGCATCTTAGCTTATTTTCTATACGTCATTTCTGGCCTAGGCATT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5887R-1.in silico 181 GCCAAGTGGCAGACGTGCATCTTAGCTTATTTTCTATACGTCATTTCTGGCCTAGGCATT 240
5887R-1.IR_full 241 ACCGCCGGTGCCCATCGTCTGTGGGCGCATCGCTCCTACAAGGCCAAGTGGCCCCTGCGA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5887R-1.in silico 241 ACCGCCGGTGCCCATCGTCTGTGGGCGCATCGCTCCTACAAGGCCAAGTGGCCCCTGCGA 300
5887R-1.IR_full 301 GTGATTCTGGTCATCTTCAACACGATTGCCTTCCAGGATGCCGCCTACCATTGGGCTCGC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5887R-1.in silico 301 GTGATTCTGGTCATCTTCAACACGATTGCCTTCCAGGATGCCGCCTACCATTGGGCTCGC 360
5887R-1.IR_full 361 GACCATCGCGTCCACCACAAATACTCGGAGACTGACGCCGATCCCCACAACGCCACGCGT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5887R-1.in silico 361 GACCATCGCGTCCACCACAAATACTCGGAGACTGACGCCGATCCCCACAACGCCACGCGT 420
5887R-1.IR_full 421 GGTTTCTTCTTCTCGCACGTCGGCTGGCTGCTGTGCAAGAAGCACCCGGAGGTGAAAGCC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5887R-1.in silico 421 GGTTTCTTCTTCTCGCACGTCGGCTGGCTGCTGTGCAAGAAGCACCCGGAGGTGAAAGCC 480
5887R-1.IR_full 481 AAGGGCAAGGGAGTCGATCT 500
||||||||||||||||||||
5887R-1.in silico 481 AAGGGCAAGGGAGTCGATCT 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
5 |
NM_144474.1 |
CG5887-RA, transcript variant A (desat1), mRNA |
| 100 |
482 |
0 |
0 |
5 |
NM_169466.1 |
CG5887-RC, transcript variant C (desat1), mRNA |
| 100 |
482 |
0 |
0 |
5 |
NM_169468.1 |
CG5887-RE, transcript variant E (desat1), mRNA |
| 100 |
482 |
0 |
0 |
5 |
NM_169465.1 |
CG5887-RB, transcript variant B (desat1), mRNA |
| 100 |
482 |
0 |
0 |
5 |
NM_169467.1 |
CG5887-RD, transcript variant D (desat1), mRNA |
| 3.73 |
18 |
46 |
69 |
53 |
NM_141944.1 |
CG5925-RA (desat2), mRNA |
| 0 |
0 |
11 |
19 |
25 |
NM_169500.1 |
CG8630-RA (CG8630), mRNA |
| 0 |
0 |
7 |
20 |
31 |
NM_143709.2 |
CG7923-RA (Fad2), mRNA |
| 0 |
0 |
4 |
20 |
43 |
NM_143522.2 |
CG9747-RA (CG9747), mRNA |
| 0 |
0 |
3 |
21 |
30 |
NM_143524.2 |
CG9743-RA (CG9743), mRNA |
| 0 |
0 |
3 |
2 |
5 |
NM_144274.2 |
CG11368-RA (CG11368), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_165891.1 |
CG30045-RA (CG30045), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_170360.1 |
CG4976-RA (Mes-4), mRNA |
| 0 |
0 |
1 |
4 |
9 |
NM_057638.3 |
CG14472-RA (poe), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_135135.3 |
CG9098-RA, transcript variant A (CG9098), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_137190.2 |
CG8174-RB, transcript variant B (SRPK), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_166093.1 |
CG8174-RA, transcript variant A (SRPK), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_136503.2 |
CG12769-RA, transcript variant A (CG12769), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_166094.1 |
CG8174-RC, transcript variant C (SRPK), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_165585.1 |
CG12769-RB, transcript variant B (CG12769), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_164672.2 |
CG9098-RB, transcript variant B (CG9098), mRNA |
| 0 |
0 |
0 |
6 |
6 |
NM_165915.1 |
CG8815-RB, transcript variant B (Sin3A), mRNA |
| 0 |
0 |
0 |
6 |
6 |
NM_165916.1 |
CG8815-RC, transcript variant C (Sin3A), mRNA |
| 0 |
0 |
0 |
6 |
6 |
NM_136955.2 |
CG8815-RA, transcript variant A (Sin3A), mRNA |
| 0 |
0 |
0 |
5 |
13 |
NM_168179.1 |
CG32394-RA (CG32394), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_142524.2 |
CG6040-RA (CG6040), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_058053.3 |
CG6939-RA, transcript variant A (Sbf), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_169430.2 |
CG6939-RB, transcript variant B (Sbf), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_080004.2 |
CG6939-RB, transcript variant B (Sbf), mRNA, ankyrin repeat, tyrosine kinase CG18247-RA (shark), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_001043133.1 |
CG32045-RD, transcript variant D (fry), mRNA |
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