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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
5475R-1 |
| Symbol |
Mpk2 |
Full Name |
Mpk2 |
| CG No |
CG5475 |
Old CG No |
CG5475 |
| Synonyms |
p38 alpha, D-p38a, P38, D-p38, p38, p38a, Dp38, p38A, CG5475, D-P38a, D-MPK2, DmMPK2, dMPK2, DMPK2, group 2, Erk2, Mpk2 |
| Accession No (Link to NCBI) |
NM_057815.3 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Gonda RL, Garlena RA, Stronach B.
Drosophila heat shock response requires the JNK pathway and phosphorylation of mixed lineage kinase at a conserved serine-proline motif.
PLoS One (2012) 7(7) e42369
[
PubMed ID = 22848763
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCCGGGCACCTGAAATAATGCT |
| Primer Seq. 3' |
aatctagaggtaccATACGATGGTGGGGGCTTAAA |
| Predicted Fragment Size |
499 |
| IR fragment full Seq |
0001 CCGCGGACCT GAAATAATGC TCAATTGGAT GcNCTaCGac CAAACAGTGG ACATCTGGTC
0061 GgtGGGCTGC ATCATGGCCG AACTAATTAC CAGACGAAcC CTCNTCCCAG GCACCGACCA
0121 TATTCACCAG CTAAACCTGA TTATGGAGAT GTTGGGCACG CCACCCGCCG AATTTTTGAA
0181 GAAGATCTCA TCGGAAAGTG CACGTTCCTA CATCCAGTCA CTTCCGCCTA TGAAGGGACG
0241 AAGTTTTAAA AATGTTTTTA AGAACGCCAA TCCGCTGGCC ATTGATTTGC TGGAAAAGAT
0301 GTTGGAGCTA GATGCCGAAA AGCGGATCAC AGCCGAGGAG GCTCTTTCCC ATCCATATCT
0361 GGAGAAGTAT GCGGAGCCCA GCGTCGAGCA GACCTCACCA CCATACGATC ACAGCTTCGA
0421 GGATATGGAT TTGCCCGTAG ACAAATGGAA GGAATTGATC TACAAGGAGG TCACCAACTT
0481 TAAGCCCCCA CCATCGTAT |
| in silico PCR Fragment |
0001 CCGGGCACCT GAAATAATGC TCAATTGGAT GCACTACGAC CAAACAGTGG ACATCTGGTC
0061 GGTGGGCTGC ATCATGGCCG AACTAATTAC CAGACGAACC CTCTTCCCAG GCACCGACCA
0121 TATTCACCAG CTAAACCTGA TTATGGAGAT GTTGGGCACG CCACCCGCCG AATTTTTGAA
0181 GAAGATCTCA TCGGAAAGTG CACGTTCCTA CATCCAGTCA CTTCCGCCTA TGAAGGGACG
0241 AAGTTTTAAA AATGTTTTTA AGAACGCCAA TCCGCTGGCC ATTGATTTGC TGGAAAAGAT
0301 GTTGGAGCTA GATGCCGAAA AGCGGATCAC AGCCGAGGAG GCTCTTTCCC ATCCATATCT
0361 GGAGAAGTAT GCGGAGCCCA GCGTCGAGCA GACCTCACCA CCATACGATC ACAGCTTCGA
0421 GGATATGGAT TTGCCCGTAG ACAAATGGAA GGAATTGATC TACAAGGAGG TCACCAACTT
0481 TAAGCCCCCA CCATCGTAT |
| Assemble Data |
5475R-1.IR_full 1 CCGCGGACCTGAAATAATGCTCAATTGGATGCNCTACGACCAAACAGTGGACATCTGGTC 60
||| | |||||||||||||||||||||||||| |||||||||||||||||||||||||||
5475R-1.in silico 1 CCGGGCACCTGAAATAATGCTCAATTGGATGCACTACGACCAAACAGTGGACATCTGGTC 60
5475R-1.IR_full 61 GGTGGGCTGCATCATGGCCGAACTAATTACCAGACGAACCCTCNTCCCAGGCACCGACCA 120
||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||
5475R-1.in silico 61 GGTGGGCTGCATCATGGCCGAACTAATTACCAGACGAACCCTCTTCCCAGGCACCGACCA 120
5475R-1.IR_full 121 TATTCACCAGCTAAACCTGATTATGGAGATGTTGGGCACGCCACCCGCCGAATTTTTGAA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5475R-1.in silico 121 TATTCACCAGCTAAACCTGATTATGGAGATGTTGGGCACGCCACCCGCCGAATTTTTGAA 180
5475R-1.IR_full 181 GAAGATCTCATCGGAAAGTGCACGTTCCTACATCCAGTCACTTCCGCCTATGAAGGGACG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5475R-1.in silico 181 GAAGATCTCATCGGAAAGTGCACGTTCCTACATCCAGTCACTTCCGCCTATGAAGGGACG 240
5475R-1.IR_full 241 AAGTTTTAAAAATGTTTTTAAGAACGCCAATCCGCTGGCCATTGATTTGCTGGAAAAGAT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5475R-1.in silico 241 AAGTTTTAAAAATGTTTTTAAGAACGCCAATCCGCTGGCCATTGATTTGCTGGAAAAGAT 300
5475R-1.IR_full 301 GTTGGAGCTAGATGCCGAAAAGCGGATCACAGCCGAGGAGGCTCTTTCCCATCCATATCT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5475R-1.in silico 301 GTTGGAGCTAGATGCCGAAAAGCGGATCACAGCCGAGGAGGCTCTTTCCCATCCATATCT 360
5475R-1.IR_full 361 GGAGAAGTATGCGGAGCCCAGCGTCGAGCAGACCTCACCACCATACGATCACAGCTTCGA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5475R-1.in silico 361 GGAGAAGTATGCGGAGCCCAGCGTCGAGCAGACCTCACCACCATACGATCACAGCTTCGA 420
5475R-1.IR_full 421 GGATATGGATTTGCCCGTAGACAAATGGAAGGAATTGATCTACAAGGAGGTCACCAACTT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5475R-1.in silico 421 GGATATGGATTTGCCCGTAGACAAATGGAAGGAATTGATCTACAAGGAGGTCACCAACTT 480
5475R-1.IR_full 481 TAAGCCCCCACCATCGTAT 499
|||||||||||||||||||
5475R-1.in silico 481 TAAGCCCCCACCATCGTAT 499
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
481 |
0 |
0 |
0 |
NM_170126.2 |
CG5475-RB, transcript variant B (Mpk2), mRNA |
| 100 |
481 |
0 |
0 |
0 |
NM_057815.3 |
CG5475-RA, transcript variant A (Mpk2), mRNA |
| 2.49 |
12 |
19 |
32 |
37 |
NM_058013.3 |
CG7393-RA (p38b), mRNA |
| 0.2 |
1 |
4 |
3 |
5 |
NM_164876.1 |
CG31711-RA (CG31711), mRNA |
| 0.2 |
1 |
2 |
2 |
2 |
NM_167288.1 |
CG32666-RB, transcript variant B (CG32666), mRNA |
| 0.2 |
1 |
2 |
2 |
2 |
NM_206688.1 |
CG32666-RA, transcript variant A (CG32666), mRNA |
| 0 |
0 |
3 |
6 |
2 |
NM_144347.2 |
CG12149-RA (c12.2), mRNA |
| 0 |
0 |
3 |
4 |
11 |
NM_001015123.1 |
CG12559-PD.3 (CG12559), mRNA |
| 0 |
0 |
3 |
4 |
10 |
NM_001015170.1 |
CG40190-PA (CG40190), mRNA |
| 0 |
0 |
3 |
4 |
10 |
NM_001015122.1 |
CG12559-PC.3 (CG12559), mRNA |
| 0 |
0 |
3 |
4 |
10 |
NM_001015121.1 |
CG12559-PB.3 (CG12559), mRNA |
| 0 |
0 |
3 |
2 |
6 |
NM_165559.1 |
CG1891-RB, transcript variant B (sax), mRNA |
| 0 |
0 |
3 |
2 |
6 |
NM_078928.2 |
CG1891-RA, transcript variant A (sax), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_169833.1 |
CG7717-RB, transcript variant B (Mekk1), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_142493.2 |
CG7717-RA, transcript variant A (Mekk1), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_058136.3 |
CG4376-RA, transcript variant A (Actn), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_058137.3 |
CG4376-RC, transcript variant C (Actn), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_166920.1 |
CG4376-RB, transcript variant B (Actn), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_170435.1 |
CG31009-RA (Cad99C), mRNA |
| 0 |
0 |
2 |
2 |
11 |
NM_167366.1 |
CG1640-RC, transcript variant C (CG1640), mRNA |
| 0 |
0 |
2 |
2 |
11 |
NM_167367.1 |
CG1640-RD, transcript variant D (CG1640), mRNA |
| 0 |
0 |
2 |
2 |
11 |
NM_167368.1 |
CG1640-RE, transcript variant E (CG1640), mRNA |
| 0 |
0 |
2 |
2 |
9 |
NM_132651.2 |
CG1640-RA, transcript variant A (CG1640), mRNA |
| 0 |
0 |
2 |
2 |
9 |
NM_167365.1 |
CG1640-RB, transcript variant B (CG1640), mRNA |
| 0 |
0 |
2 |
2 |
9 |
NM_167369.1 |
CG1640-RF, transcript variant F (CG1640), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_167597.1 |
CG32494-RA (CG32494), mRNA |
| 0 |
0 |
1 |
7 |
3 |
NM_132772.2 |
CG5599-RA (CG5599), mRNA |
| 0 |
0 |
1 |
5 |
5 |
NM_168869.1 |
CG4268-RC, transcript variant C (Pitslre), mRNA |
| 0 |
0 |
1 |
5 |
5 |
NM_140994.2 |
CG4268-RA, transcript variant A (Pitslre), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_001043286.1 |
CG6383-RB, transcript variant B (crb), mRNA |
|
| Wing Disc |
GFP
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Puc
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Caspase
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DAPI
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Merge
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Figure
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JNK signal
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Caspase-3 signal
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