NIG-Fly - Fly Stocks of National Institute of Genetics -

Notice:
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.

NIG-RNAi Strains Detail

Request : Click the "Order" button to request for this stock

discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 4584R-3

Symbol dUTPase Full Name Deoxyuridine triphosphatase

CG No CG4584 Old CG No CG4584

Synonyms CG4584, LD08534, BcDNA:LD08534, UTPase, dUTPase

Accession No (Link to NCBI) NM_135635.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Apidianakis Y, Mindrinos MN, Xiao W, Tegos GP, Papisov MI, Hamblin MR, Davis RW, Tompkins RG, Rahme LG.
Involvement of skeletal muscle gene regulatory network in susceptibility to wound infection following trauma.
PLoS One (2007) 2(12) e1356 [ PubMed ID = 18159239 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGCCATCAACCGATTTCGCC

Primer Seq. 3'

aatctagaggtaccTTGGCTGCCGGCAGATCCTTG

Predicted Fragment Size

IR fragment full Seq

0001 ATGCCATCAA CCGATTTCGC CGACATTCCA GCTGCCAAGA AGATGAAGAT CGACACGTGC 
0061 GTCCTGCGAT TCGCCAAACT CACCGAGAAT GCTTTGGAGC CGGTGAGGGG ATCCGCCAAA 
0121 GCGGCGGGAG TTGACCTGCG CAGCGCCTAC GACGTTGTGG TGCCGGCACG CGGNAAAGGC 
0181 CATTGTCAAG ACCGATCTGC AAGTGCAGGT TCCGGAGGGC TCCTACGGAN CGCGTAGCCC 
0241 CACGATCCGG GCTGGCGGTG AAGAACTTCA TTGATGTGGG CGCCGGTGTG GTGGACGAGG 
0301 ATTATCGCGG CAATCTCGGC GTCGTCCTGT TCAATCACTC AGATGTTGAT TTCGAGGTGA 
0361 AGCATGGNGA CCGCATCGCC CAGTTCATTT GCGAGCGTAT CTTCTATCCG CAACTGGTGA 
0421 TGGTGGACAA ACTGGAGGAC ACCGAGCGCG GCGAGGCAGG ATTCGGTTCC ACCGGAGTCA 
0481 AGGATTCTGC CGGCAGCCAa

in silico PCR Fragment

0001 ATGCCATCAA CCGATTTCGC CGACATTCCA GCTGCCAAGA AGATGAAGAT CGACACGTGC 
0061 GTCCTGCGAT TCGCCAAACT CACCGAGAAT GCTTTGGAGC CGGTGAGGGG ATCCGCCAAA 
0121 GCGGCGGGAG TTGACCTGCG CAGCGCCTAC GACGTTGTGG TGCCGGCACG CGG-AAAGGC 
0181 CATTGTCAAG ACCGATCTGC AAGTGCAGGT TCCGGAGGGC TCCTACGGA- CGCGTAGCCC 
0241 CACGATCCGG GCTGGCGGTG AAGAACTTCA TTGATGTGGG CGCCGGTGTG GTGGACGAGG 
0301 ATTATCGCGG CAATCTCGGC GTCGTCCTGT TCAATCACTC AGATGTTGAT TTCGAGGTGA 
0361 AGCATGGCGA CCGCATCGCC CAGTTCATTT GCGAGCGTAT CTTCTATCCG CAACTGGTGA 
0421 TGGTGGACAA ACTGGAGGAC ACCGAGCGCG GCGAGGCAGG ATTCGGTTCC ACCGGAGTCA 
0481 AGGA-TCTGC CGGCAGCCAA

Assemble Data


4584R-3.IR_full       1   ATGCCATCAACCGATTTCGCCGACATTCCAGCTGCCAAGAAGATGAAGATCGACACGTGC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4584R-3.in silico     1   ATGCCATCAACCGATTTCGCCGACATTCCAGCTGCCAAGAAGATGAAGATCGACACGTGC 60


4584R-3.IR_full       61  GTCCTGCGATTCGCCAAACTCACCGAGAATGCTTTGGAGCCGGTGAGGGGATCCGCCAAA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4584R-3.in silico     61  GTCCTGCGATTCGCCAAACTCACCGAGAATGCTTTGGAGCCGGTGAGGGGATCCGCCAAA 120


4584R-3.IR_full       121 GCGGCGGGAGTTGACCTGCGCAGCGCCTACGACGTTGTGGTGCCGGCACGCGGNAAAGGC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||
4584R-3.in silico     121 GCGGCGGGAGTTGACCTGCGCAGCGCCTACGACGTTGTGGTGCCGGCACGCGG-AAAGGC 180


4584R-3.IR_full       181 CATTGTCAAGACCGATCTGCAAGTGCAGGTTCCGGAGGGCTCCTACGGANCGCGTAGCCC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||
4584R-3.in silico     181 CATTGTCAAGACCGATCTGCAAGTGCAGGTTCCGGAGGGCTCCTACGGA-CGCGTAGCCC 240


4584R-3.IR_full       241 CACGATCCGGGCTGGCGGTGAAGAACTTCATTGATGTGGGCGCCGGTGTGGTGGACGAGG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4584R-3.in silico     241 CACGATCCGGGCTGGCGGTGAAGAACTTCATTGATGTGGGCGCCGGTGTGGTGGACGAGG 300


4584R-3.IR_full       301 ATTATCGCGGCAATCTCGGCGTCGTCCTGTTCAATCACTCAGATGTTGATTTCGAGGTGA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4584R-3.in silico     301 ATTATCGCGGCAATCTCGGCGTCGTCCTGTTCAATCACTCAGATGTTGATTTCGAGGTGA 360


4584R-3.IR_full       361 AGCATGGNGACCGCATCGCCCAGTTCATTTGCGAGCGTATCTTCTATCCGCAACTGGTGA 420
                          ||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||
4584R-3.in silico     361 AGCATGGCGACCGCATCGCCCAGTTCATTTGCGAGCGTATCTTCTATCCGCAACTGGTGA 420


4584R-3.IR_full       421 TGGTGGACAAACTGGAGGACACCGAGCGCGGCGAGGCAGGATTCGGTTCCACCGGAGTCA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4584R-3.in silico     421 TGGTGGACAAACTGGAGGACACCGAGCGCGGCGAGGCAGGATTCGGTTCCACCGGAGTCA 480


4584R-3.IR_full       481 AGGATTCTGCCGGCAGCCAA 500
                          |||| |||||||||||||||
4584R-3.in silico     481 AGGA-TCTGCCGGCAGCCAA 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	479	0	0	0	NM_135635.2	CG4584-RA, transcript variant A (dUTPase), mRNA
93.52	448	3	2	1	NM_164956.1	CG4584-RB, transcript variant B (dUTPase), mRNA
0	0	3	3	4	NM_132826.2	CG9240-RA (CG9240), mRNA
0	0	3	2	5	NM_132734.2	CG9411-RA (CG9411), mRNA
0	0	2	6	7	NM_135922.2	CG3793-RA (CG3793), mRNA
0	0	2	4	2	NM_132881.2	CG3415-RA (CG3415), mRNA
0	0	2	3	2	NM_143677.2	CG11360-RA (CG11360), mRNA
0	0	2	3	2	NM_057849.3	CG3733-RA (Chd1), mRNA
0	0	2	2	4	NM_080263.2	CG1274-RA, transcript variant A (Jafrac2), mRNA
0	0	2	2	4	NM_167971.1	CG1274-RB, transcript variant B (Jafrac2), mRNA
0	0	1	5	4	NM_132370.1	CG2989-RA (CG2989), mRNA
0	0	1	5	3	NM_130507.2	CG7359-RA (CG7359), mRNA
0	0	1	4	4	NM_132838.2	CG8231-RA (l(1)G0022), mRNA
0	0	1	4	4	NM_166385.2	CG8908-RA, transcript variant A (CG8908), mRNA
0	0	1	4	4	NM_137620.2	CG8908-RB, transcript variant B (CG8908), mRNA
0	0	1	3	6	NM_143367.2	CG10011-RA (CG10011), mRNA
0	0	1	3	4	NM_169232.1	CG8036-RD, transcript variant D (CG8036), mRNA
0	0	1	3	4	NM_169231.1	CG8036-RC, transcript variant C (CG8036), mRNA
0	0	1	3	4	NM_141555.1	CG8036-RB, transcript variant B (CG8036), mRNA
0	0	1	3	4	NM_136515.2	CG8709-RA (CG8709), mRNA
0	0	1	3	3	NM_143553.1	CG9702-RA (CG9702), mRNA
0	0	1	3	3	NM_176547.1	CG31145-RB, transcript variant B (CG31145), mRNA
0	0	1	3	3	NM_170079.2	CG31145-RA, transcript variant A (CG31145), mRNA
0	0	1	3	2	NM_080178.4	CG7899-RA, transcript variant A (Acph-1), mRNA
0	0	1	3	2	NM_170453.1	CG7899-RB, transcript variant B (Acph-1), mRNA
0	0	1	2	2	NM_132687.1	CG9941-RA (CG9941), mRNA
0	0	0	6	3	NM_165723.2	CG15862-RB, transcript variant B (Pka-R2), mRNA
0	0	0	6	3	NM_078947.2	CG15862-RA, transcript variant A (Pka-R2), mRNA
0	0	0	6	3	NM_176117.1	CG15862-RC, transcript variant C (Pka-R2), mRNA
0	0	0	5	18	NM_175960.3	CG33196-RB (dp), mRNA

Back