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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4316R-1 |
| Symbol |
Sb |
Full Name |
Stubble |
| CG No |
CG4316 |
Old CG No |
CG4316 |
| Synonyms |
sbd, SP56, c-SP56, st-sb, Sb-sbd, CG4316, Sb |
| Accession No (Link to NCBI) |
NM_057361.3 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
PLoS Genet (2019) 15(4) e1008121
[
PubMed ID = 31034473
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Ibrahim DM, Biehs B, Kornberg TB, Klebes A.
Microarray comparison of anterior and posterior Drosophila wing imaginal disc cells identifies novel wing genes.
G3 (Bethesda) (2013) 3(8) 1353-62
[
PubMed ID = 23749451
]
[
RRC reference
]
Kambris Z, Brun S, Jang IH, Nam HJ, Romeo Y, Takahashi K, Lee WJ, Ueda R, Lemaitre B.
Drosophila immunity: a large-scale in vivo RNAi screen identifies five serine proteases required for Toll activation.
Curr Biol (2006) 16(8) 808-13
[
PubMed ID = 16631589
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAGTAGCGGCTTCTACCGCATC |
| Primer Seq. 3' |
aatctagaggtaccGAGCTGGAACTGGAGCTGGGA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AGTAGCGGCT TCTACCGCAT CCCGCACCGC CTGGAgGGNT ATCCGCAGTT GCAGCAACTG
0061 CAGCGCGGCC AGAACTTCAA GATCAGCCCC AAGCCATGCT CCTTTGGCCG CGTCGAGGGC
0121 ACCTGCATGT TCGTGTGGGA GTGCATCAAG TCCGAGGGCA AGCACGTGGG CATGTGCGTC
0181 GACTCCTTCA TGTTCGGCTC CTGCTGCACG CACAACTACA CCGACAACAT TGTCCTGCCC
0241 CAGACGGCCT TCTCCTACAC GAGGCCCACC ANAGCCGCTC ACGCTCCGCC CGCGACCGCC
0301 GGCAGCGCCC TACAAGCCGA TGATCAGCGG CATGACCACC ATCGAGAGGC CTCATGGCGC
0361 TGGCACCCTT GTGATTCGTC CTTCGGGTCC GCACCACCAG GGCACTCTGG CCCGCCCGCA
0421 TCCGCCGCCC TACCAGAGCA AGCCCACCAC TGCCTCGGAT CTGCATGGCT CAGCNTCGCA
0481 TCCCAGCTCC AGTTCCAGCT c |
| in silico PCR Fragment |
0001 AGTAGCGGCT TCTACCGCAT CCCGCACCGC CTGGAGGGCT ATCCGCAGTT GCAGCAACTG
0061 CAGCGCGGCC AGAACTTCAA GATCAGCCCC AAGCCATGCT CCTTTGGCCG CGTCGAGGGC
0121 ACCTGCATGT TCGTGTGGGA GTGCATCAAG TCCGAGGGCA AGCACGTGGG CATGTGCGTC
0181 GACTCCTTCA TGTTCGGCTC CTGCTGCACG CACAACTACA CCGACAACAT TGTCCTGCCC
0241 CAGACGGCCT TCTCCTACAC GAGGCCCACC A-AGCCGCTC ACGCTCCGCC CGCGACCGCC
0301 GGCAGCGCCC TACAAGCCGA TGATCAGCGG CATGACCACC ATCGAGAGGC CTCATGGCGC
0361 TGGCACCCTT GTGATTCGTC CTTCGGGTCC GCACCACCAG GGCACTCTGG CCCGCCCGCA
0421 TCCGCCGCCC TACCAGAGCA AGCCCACCAC TGCCTCGGAT CTGCATGGCT CAGCCTCGCA
0481 TCCCAGCTCC AGTTCCAGCT C |
| Assemble Data |
4316R-1.IR_full 1 AGTAGCGGCTTCTACCGCATCCCGCACCGCCTGGAGGGNTATCCGCAGTTGCAGCAACTG 60
|||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||
4316R-1.in silico 1 AGTAGCGGCTTCTACCGCATCCCGCACCGCCTGGAGGGCTATCCGCAGTTGCAGCAACTG 60
4316R-1.IR_full 61 CAGCGCGGCCAGAACTTCAAGATCAGCCCCAAGCCATGCTCCTTTGGCCGCGTCGAGGGC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4316R-1.in silico 61 CAGCGCGGCCAGAACTTCAAGATCAGCCCCAAGCCATGCTCCTTTGGCCGCGTCGAGGGC 120
4316R-1.IR_full 121 ACCTGCATGTTCGTGTGGGAGTGCATCAAGTCCGAGGGCAAGCACGTGGGCATGTGCGTC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4316R-1.in silico 121 ACCTGCATGTTCGTGTGGGAGTGCATCAAGTCCGAGGGCAAGCACGTGGGCATGTGCGTC 180
4316R-1.IR_full 181 GACTCCTTCATGTTCGGCTCCTGCTGCACGCACAACTACACCGACAACATTGTCCTGCCC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4316R-1.in silico 181 GACTCCTTCATGTTCGGCTCCTGCTGCACGCACAACTACACCGACAACATTGTCCTGCCC 240
4316R-1.IR_full 241 CAGACGGCCTTCTCCTACACGAGGCCCACCANAGCCGCTCACGCTCCGCCCGCGACCGCC 300
||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||
4316R-1.in silico 241 CAGACGGCCTTCTCCTACACGAGGCCCACCA-AGCCGCTCACGCTCCGCCCGCGACCGCC 300
4316R-1.IR_full 301 GGCAGCGCCCTACAAGCCGATGATCAGCGGCATGACCACCATCGAGAGGCCTCATGGCGC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4316R-1.in silico 301 GGCAGCGCCCTACAAGCCGATGATCAGCGGCATGACCACCATCGAGAGGCCTCATGGCGC 360
4316R-1.IR_full 361 TGGCACCCTTGTGATTCGTCCTTCGGGTCCGCACCACCAGGGCACTCTGGCCCGCCCGCA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4316R-1.in silico 361 TGGCACCCTTGTGATTCGTCCTTCGGGTCCGCACCACCAGGGCACTCTGGCCCGCCCGCA 420
4316R-1.IR_full 421 TCCGCCGCCCTACCAGAGCAAGCCCACCACTGCCTCGGATCTGCATGGCTCAGCNTCGCA 480
|||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
4316R-1.in silico 421 TCCGCCGCCCTACCAGAGCAAGCCCACCACTGCCTCGGATCTGCATGGCTCAGCCTCGCA 480
4316R-1.IR_full 481 TCCCAGCTCCAGTTCCAGCTC 501
|||||||||||||||||||||
4316R-1.in silico 481 TCCCAGCTCCAGTTCCAGCTC 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
3 |
14 |
NM_057361.3 |
CG4316-RA (Sb), mRNA |
| 0.2 |
1 |
1 |
1 |
4 |
NM_058149.3 |
CG3352-RA (ft), mRNA |
| 0 |
0 |
9 |
5 |
11 |
NM_130640.1 |
CG2879-RA (CG2879), mRNA |
| 0 |
0 |
6 |
7 |
14 |
NM_131995.1 |
CG15784-RA (CG15784), mRNA |
| 0 |
0 |
6 |
5 |
8 |
NM_140744.1 |
CG6064-RA (TORC), mRNA |
| 0 |
0 |
4 |
4 |
6 |
NM_132443.1 |
CG16922-RA (CG16922), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_140249.1 |
CG14131-RA (CG14131), mRNA |
| 0 |
0 |
3 |
5 |
13 |
NM_170420.1 |
CG18741-RA, transcript variant A (DopR2), mRNA |
| 0 |
0 |
3 |
5 |
11 |
NM_079824.2 |
CG18741-RB, transcript variant B (DopR2), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_144343.2 |
CG8250-RA (Alk), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_139501.2 |
CG2114-RA (FR), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_168794.2 |
CG32206-RB, transcript variant B (CG32206), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_176360.1 |
CG32206-RC, transcript variant C (CG32206), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_079375.2 |
CG5949-RA (DNApol-delta), mRNA |
| 0 |
0 |
3 |
1 |
5 |
NM_170325.1 |
CG31064-RA, transcript variant A (CG31064), mRNA |
| 0 |
0 |
3 |
1 |
5 |
NM_143288.2 |
CG31064-RE, transcript variant E (CG31064), mRNA |
| 0 |
0 |
2 |
5 |
7 |
NM_001038767.1 |
CG34019-RA (CG34019), mRNA |
| 0 |
0 |
2 |
5 |
4 |
NM_165643.1 |
CG8073-RB, transcript variant B (Pmm45A), mRNA |
| 0 |
0 |
2 |
5 |
4 |
NM_136609.3 |
CG8073-RA, transcript variant A (Pmm45A), mRNA |
| 0 |
0 |
2 |
4 |
16 |
NM_001014624.1 |
CG33555-RC, transcript variant C (btsz), mRNA |
| 0 |
0 |
2 |
4 |
14 |
NM_001014623.1 |
CG33555-RD, transcript variant D (btsz), mRNA |
| 0 |
0 |
2 |
4 |
9 |
NM_139482.1 |
CG14950-RA (CG14950), mRNA |
| 0 |
0 |
2 |
3 |
11 |
NM_078797.2 |
CG13109-RA (tai), mRNA |
| 0 |
0 |
2 |
3 |
8 |
NM_135859.1 |
CG17341-RA (CG17341), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_130546.2 |
CG11409-RB (CG11409), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_140919.1 |
CG7385-RA (CG7385), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_132544.1 |
CG18130-RA (CG18130), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_058023.4 |
CG4900-RA (Irp-1A), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_144447.1 |
CG18870-RA (CG18870), mRNA |
| 0 |
0 |
2 |
2 |
12 |
NM_078740.2 |
CG8817-RB, transcript variant B (lilli), mRNA |
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