|
Notice:
|
|
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
3936R-2 |
| Symbol |
N |
Full Name |
Notch |
| CG No |
CG3936 |
Old CG No |
CG3936 |
| Synonyms |
notch, CG3936, EG:163A10.2, CT13012, dNotch, swb, shd, nd, Co, Chp, Ax, spl, fa, 1.1, clone 1.12, n[fah], co, 16-55, 16-178, l(1)N, l(1)Ax, l(1)3Cb, anon-EST:Liang-1.12, EG:140G11.1, N, Notch |
| Accession No (Link to NCBI) |
NM_057511.3 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Ibaraki K, Nakatsuka M, Ohsako T, Watanabe M, Miyazaki Y, Shirakami M, Karr TL, Sanuki R, Tomaru M, Takano-Shimizu-Kouno T.
A cross-species approach for the identification of Drosophila male sterility genes.
G3 (Bethesda) (2021) 11(8)
[
PubMed ID = 34849808
]
[
RRC reference
]
Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Fairchild MJ, Islam F, Tanentzapf G.
Identification of genetic networks that act in the somatic cells of the testis to mediate the developmental program of spermatogenesis.
PLoS Genet (2017) 13(9) e1007026
[
PubMed ID = 28957323
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Rao PR, Lin L, Huang H, Guha A, Roy S, Kornberg TB.
Developmental compartments in the larval trachea of Drosophila.
Elife (2015) 4
[
PubMed ID = 26491942
]
[
RRC reference
]
Perkins AD, Lee MJ, Tanentzapf G.
The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance.
Sci Data (2014) 1 140002
[
PubMed ID = 25977760
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Lin G, Zhang X, Ren J, Pang Z, Wang C, Xu N, Xi R.
Integrin signaling is required for maintenance and proliferation of intestinal stem cells in Drosophila.
Dev Biol (2013) 377(1) 177-87
[
PubMed ID = 23410794
]
[
RRC reference
]
Casso DJ, Biehs B, Kornberg TB.
A novel interaction between hedgehog and Notch promotes proliferation at the anterior-posterior organizer of the Drosophila wing.
Genetics (2011) 187(2) 485-99
[
PubMed ID = 21098717
]
[
RRC reference
]
Mummery-Widmer JL, Yamazaki M, Stoeger T, Novatchkova M, Bhalerao S, Chen D, Dietzl G, Dickson BJ, Knoblich JA.
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.
Nature (2009) 458(7241) 987-92
[
PubMed ID = 19363474
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgACTGGAGGAGTACACGTGTGC |
| Primer Seq. 3' |
aatctagaggtaccCCGTGAAGTTCGGTGGACAGC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ACTGGAGGAG TACACGTGTG CGTGTGCCAA TGGCTATACA GGTGAACGAT GCGAGACGAA
0061 GAATCTGTGC GCAAGTTTCT CCCTGCCGGA ATGGAGCCAC CTGCACCGCT TTGGCCGGAA
0121 GCAGCAGCTT CACCTGCTCC TGTCCGCCGG GCTTCACCGG TGACACCTGT TCCTATGACA
0181 TCGAGGAGTG CCAGTCGAAT CCGTGCAAAT ACGGCGGCAC ATGTGTCAAC ACCCACGGAT
0241 CTTACCAGNG CATGTGTcCC ACGGGCTACA CGGGAAAGGA TTGCGACACC AAGTACAAGC
0301 CCTGCTCGCC ATCGCCGTGC CAGAATGGCG GAATTTGCCG GTCGAACGGT CTGTCCTACG
0361 AGTGCAAGTG CCCCAAAGGT TTCGAGGGCA AGAACTGCGA ACAGAACTAC GACGATTGCC
0421 TGGGACATCT TTGCCAGAAC GGAGGAACCT GCATCGATGG CATCTCGGAC TACACCTGCC
0481 GCTGTCCACC GAACTTCACG G |
| in silico PCR Fragment |
0001 ACTGGAGGAG TACACGTGTG CGTGTGCCAA TGGCTATACA GGTGAACGAT GCGAGACGAA
0061 GAATCTGTGC GCAAG-TTCT CCCTGCCGGA ATGGAGCCAC CTGCACCGCT TTGGCCGGAA
0121 GCAGCAGCTT CACCTGCTCC TGTCCGCCGG GCTTCACCGG TGACACCTGT TCCTATGACA
0181 TCGAGGAGTG CCAGTCGAAT CCGTGCAAAT ACGGCGGCAC ATGTGTCAAC ACCCACGGAT
0241 CTTACCAGTG CATGTGTCCC ACGGGCTACA CGGGAAAGGA TTGCGACACC AAGTACAAGC
0301 CCTGCTCGCC ATCGCCGTGC CAGAATGGCG GAATTTGCCG GTCGAACGGT CTGTCCTACG
0361 AGTGCAAGTG CCCCAAAGGT TTCGAGGGCA AGAACTGCGA ACAGAACTAC GACGATTGCC
0421 TGGGACATCT TTGCCAGAAC GGAGGAACCT GCATCGATGG CATCTCGGAC TACACCTGCC
0481 GCTGTCCACC GAACTTCACG G |
| Assemble Data |
3936R-2.IR_full 1 ACTGGAGGAGTACACGTGTGCGTGTGCCAATGGCTATACAGGTGAACGATGCGAGACGAA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3936R-2.in silico 1 ACTGGAGGAGTACACGTGTGCGTGTGCCAATGGCTATACAGGTGAACGATGCGAGACGAA 60
3936R-2.IR_full 61 GAATCTGTGCGCAAGTTTCTCCCTGCCGGAATGGAGCCACCTGCACCGCTTTGGCCGGAA 120
||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||
3936R-2.in silico 61 GAATCTGTGCGCAAG-TTCTCCCTGCCGGAATGGAGCCACCTGCACCGCTTTGGCCGGAA 120
3936R-2.IR_full 121 GCAGCAGCTTCACCTGCTCCTGTCCGCCGGGCTTCACCGGTGACACCTGTTCCTATGACA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3936R-2.in silico 121 GCAGCAGCTTCACCTGCTCCTGTCCGCCGGGCTTCACCGGTGACACCTGTTCCTATGACA 180
3936R-2.IR_full 181 TCGAGGAGTGCCAGTCGAATCCGTGCAAATACGGCGGCACATGTGTCAACACCCACGGAT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3936R-2.in silico 181 TCGAGGAGTGCCAGTCGAATCCGTGCAAATACGGCGGCACATGTGTCAACACCCACGGAT 240
3936R-2.IR_full 241 CTTACCAGNGCATGTGTCCCACGGGCTACACGGGAAAGGATTGCGACACCAAGTACAAGC 300
|||||||| |||||||||||||||||||||||||||||||||||||||||||||||||||
3936R-2.in silico 241 CTTACCAGTGCATGTGTCCCACGGGCTACACGGGAAAGGATTGCGACACCAAGTACAAGC 300
3936R-2.IR_full 301 CCTGCTCGCCATCGCCGTGCCAGAATGGCGGAATTTGCCGGTCGAACGGTCTGTCCTACG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3936R-2.in silico 301 CCTGCTCGCCATCGCCGTGCCAGAATGGCGGAATTTGCCGGTCGAACGGTCTGTCCTACG 360
3936R-2.IR_full 361 AGTGCAAGTGCCCCAAAGGTTTCGAGGGCAAGAACTGCGAACAGAACTACGACGATTGCC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3936R-2.in silico 361 AGTGCAAGTGCCCCAAAGGTTTCGAGGGCAAGAACTGCGAACAGAACTACGACGATTGCC 420
3936R-2.IR_full 421 TGGGACATCTTTGCCAGAACGGAGGAACCTGCATCGATGGCATCTCGGACTACACCTGCC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3936R-2.in silico 421 TGGGACATCTTTGCCAGAACGGAGGAACCTGCATCGATGGCATCTCGGACTACACCTGCC 480
3936R-2.IR_full 481 GCTGTCCACCGAACTTCACGG 501
|||||||||||||||||||||
3936R-2.in silico 481 GCTGTCCACCGAACTTCACGG 501
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
5 |
35 |
140 |
NM_057511.3 |
CG3936-RA (N), mRNA |
| 0.82 |
4 |
4 |
7 |
33 |
NM_079803.2 |
CG6127-RA (Ser), mRNA |
| 0 |
0 |
5 |
5 |
9 |
NM_057442.3 |
CG15288-RA, transcript variant A (wb), mRNA |
| 0 |
0 |
5 |
5 |
9 |
NM_165086.1 |
CG15288-RB, transcript variant B (wb), mRNA |
| 0 |
0 |
4 |
4 |
2 |
NM_164770.1 |
CG7196-RB, transcript variant B (CG7196), mRNA |
| 0 |
0 |
4 |
4 |
2 |
NM_135301.2 |
CG7196-RA, transcript variant A (CG7196), mRNA |
| 0 |
0 |
3 |
4 |
20 |
NM_206292.1 |
CG7526-RB, transcript variant B (CG7526), mRNA |
| 0 |
0 |
3 |
4 |
20 |
NM_139880.2 |
CG7526-RA, transcript variant A (CG7526), mRNA |
| 0 |
0 |
3 |
4 |
5 |
NM_137035.1 |
CG6061-RA (mip120), mRNA |
| 0 |
0 |
2 |
17 |
84 |
NM_175960.3 |
CG33196-RB (dp), mRNA |
| 0 |
0 |
2 |
6 |
13 |
NM_168478.1 |
CG32094-RA (CG32094), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_135336.2 |
CG7466-RA (CG7466), mRNA |
| 0 |
0 |
2 |
2 |
32 |
NM_176104.1 |
CG33087-RC (CG33087), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_142259.2 |
CG10278-RA (GATAe), mRNA |
| 0 |
0 |
1 |
10 |
30 |
NM_169853.1 |
CG3619-RB, transcript variant B (Dl), mRNA |
| 0 |
0 |
1 |
10 |
30 |
NM_057916.3 |
CG3619-RA, transcript variant A (Dl), mRNA |
| 0 |
0 |
1 |
8 |
55 |
NM_001043286.1 |
CG6383-RB, transcript variant B (crb), mRNA |
| 0 |
0 |
1 |
8 |
55 |
NM_079756.2 |
CG6383-RA, transcript variant A (crb), mRNA |
| 0 |
0 |
1 |
6 |
2 |
NM_168019.2 |
CG32279-RA (dro2), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_167938.1 |
CG9018-RB, transcript variant B (CG9018), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_139444.1 |
CG9018-RA, transcript variant A (CG9018), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_141723.1 |
CG6208-RA (CG6208), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_143104.1 |
CG11856-RA (Nup358), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_168980.1 |
CG11100-RC, transcript variant C (Mes2), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_168979.2 |
CG11100-RB, transcript variant B (Mes2), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_139877.1 |
CG16998-RA (CG16998), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_169114.1 |
CG1213-RC, transcript variant C (CG1213), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_166257.1 |
CG5785-RB (thr), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_143558.1 |
CG2267-RA (CG2267), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_141702.2 |
CG12806-RA (Teh1), mRNA |
|
|
|
