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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
3722R-2 |
| Symbol |
shg |
Full Name |
shotgun |
| CG No |
CG3722 |
Old CG No |
CG3722 |
| Synonyms |
E-cad, E-Cad, Ecad, DCAD2, DE-cadherin, DE-Cad, E-cadherin, DE-cad, Cad, Shg, CT12481, shg/DE-Cad, DE, DE[cyto], DE-CAD, CADH, DCad, DEC, l(2)k10220, l(2)k03401, DECad, D-cad, gp150, l(2)10469, CadE, CG3722, shg, DE-Cadherin, e-cad, DEcad, ECad |
| Accession No (Link to NCBI) |
NM_057374.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Minami R, Sato C, Yamahama Y, Kubo H, Hariyama T, Kimura KI.
An RNAi Screen for Genes Involved in Nanoscale Protrusion Formation on Corneal Lens in Drosophila melanogaster.
Zoolog Sci (2016) 33(6) 583-591
[
PubMed ID = 27927092
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Ito S, Ueda T, Ueno A, Nakagawa H, Taniguchi H, Kayukawa N, Miki T.
A genetic screen in Drosophila for regulators of human prostate cancer progression.
Biochem Biophys Res Commun (2014) 451(4) 548-55
[
PubMed ID = 25117438
]
[
RRC reference
]
Campbell K, Casanova J, Skaer H.
Mesenchymal-to-epithelial transition of intercalating cells in Drosophila renal tubules depends on polarity cues from epithelial neighbours.
Mech Dev (2010) 127(7-8) 345-57
[
PubMed ID = 20382220
]
[
RRC reference
]
Wang C, Zhao R, Huang P, Yang F, Quan Z, Xu N, Xi R.
APC loss-induced intestinal tumorigenesis in Drosophila: Roles of Ras in Wnt signaling activation and tumor progression.
Dev Biol (2013) 378(2) 122-40
[
PubMed ID = 23570874
]
[
RRC reference
]
Maeda K, Takemura M, Umemori M, Adachi-Yamada T.
E-cadherin prolongs the moment for interaction between intestinal stem cell and its progenitor cell to ensure Notch signaling in adult Drosophila midgut.
Genes Cells (2008) 13(12) 1219-27
[
PubMed ID = 19021776
]
[
RRC reference
]
Mummery-Widmer JL, Yamazaki M, Stoeger T, Novatchkova M, Bhalerao S, Chen D, Dietzl G, Dickson BJ, Knoblich JA.
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.
Nature (2009) 458(7241) 987-92
[
PubMed ID = 19363474
]
[
RRC reference
]
Sasaki N, Sasamura T, Ishikawa HO, Kanai M, Ueda R, Saigo K, Matsuno K.
Polarized exocytosis and transcytosis of Notch during its apical localization in Drosophila epithelial cells.
Genes Cells (2007) 12(1) 89-103
[
PubMed ID = 17212657
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCCAGAAGCTACCACTGCATCA |
| Primer Seq. 3' |
aatctagaggtaccCAGTGGGGGCAGACCATTATC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CCAGAAGCTA CCACTGCATC AATATGTCGG CGACCCCGCA GGCCGGCCAC CTAAATCCCG
0061 CCCAGCAACA GACGCACCAG CAGCACAAGC GAAAATGCCG CGACCTTGGC AGGCGACTAA
0121 TCCCCGCGAG ACTCCTCCTG GGCGTTATCG TGGCCATCAG CCTTCTGTCA CCCGCCCTCG
0181 CACTTCACTC GCCGCCGGAC AAAAACTTTT CCGGCGACAA TCGTAAGCCC GCCTTCAAAA
0241 ACTGCGCCGG ATACGCTCCT AAGGTCAAAG AGGAGCAGCC CGAAAACACC TACGTTCTCA
0301 CCGTAGAGGC CGTCGACCCT GATCCCGACC AGGTCATTCG ATACAGCATC GTCCAGTCGC
0361 CGTTCGAGCG ACCCAAGTTC TTTATCAATC CCAGCACGGG CGTAATCTTC ACCACCCACA
0421 CATTCGATCG TGACGAGCCG ATCCACGAGA AGTTCGTCTT CGTCACCGTC CAGGCCACGG
0481 ATAATGGTCT GCCCCCACTT G |
| in silico PCR Fragment |
0001 CCAGAAGCTA CCACTGCATC AATATGTCGG CGACCCCGCA GGCCGGCCAC CTAAATCCCG
0061 CCCAGCAACA GACGCACCAG CAGCACAAGC GAAAATGCCG CGACCTTGGC AGGCGACTAA
0121 TCCCCGCGAG ACTCCTCCTG GGCGTTATCG TGGCCATCAG CCTTCTGTCA CCCGCCCTCG
0181 CACTTCACTC GCCGCCGGAC AAAAACTTTT CCGGCGACAA TCGTAAGCCC GCCTTCAAAA
0241 ACTGCGCCGG ATACGCTCCT AAGGTCAAAG AGGAGCAGCC CGAAAACACC TACGTTCTCA
0301 CCGTAGAGGC CGTCGACCCT GATCCCGACC AGGTCATTCG ATACAGCATC GTCCAGTCGC
0361 CGTTCGAGCG ACCCAAGTTC TTTATCAATC CCAGCACGGG CGTAATCTTC ACCACCCACA
0421 CATTCGATCG TGACGAGCCG ATCCACGAGA AGTTCGTCTT CGTCACCGTC CAGGCCACGG
0481 ATAATGGTCT GCCCCCAC-T G |
| Assemble Data |
3722R-2.IR_full 1 CCAGAAGCTACCACTGCATCAATATGTCGGCGACCCCGCAGGCCGGCCACCTAAATCCCG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3722R-2.in silico 1 CCAGAAGCTACCACTGCATCAATATGTCGGCGACCCCGCAGGCCGGCCACCTAAATCCCG 60
3722R-2.IR_full 61 CCCAGCAACAGACGCACCAGCAGCACAAGCGAAAATGCCGCGACCTTGGCAGGCGACTAA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3722R-2.in silico 61 CCCAGCAACAGACGCACCAGCAGCACAAGCGAAAATGCCGCGACCTTGGCAGGCGACTAA 120
3722R-2.IR_full 121 TCCCCGCGAGACTCCTCCTGGGCGTTATCGTGGCCATCAGCCTTCTGTCACCCGCCCTCG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3722R-2.in silico 121 TCCCCGCGAGACTCCTCCTGGGCGTTATCGTGGCCATCAGCCTTCTGTCACCCGCCCTCG 180
3722R-2.IR_full 181 CACTTCACTCGCCGCCGGACAAAAACTTTTCCGGCGACAATCGTAAGCCCGCCTTCAAAA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3722R-2.in silico 181 CACTTCACTCGCCGCCGGACAAAAACTTTTCCGGCGACAATCGTAAGCCCGCCTTCAAAA 240
3722R-2.IR_full 241 ACTGCGCCGGATACGCTCCTAAGGTCAAAGAGGAGCAGCCCGAAAACACCTACGTTCTCA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3722R-2.in silico 241 ACTGCGCCGGATACGCTCCTAAGGTCAAAGAGGAGCAGCCCGAAAACACCTACGTTCTCA 300
3722R-2.IR_full 301 CCGTAGAGGCCGTCGACCCTGATCCCGACCAGGTCATTCGATACAGCATCGTCCAGTCGC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3722R-2.in silico 301 CCGTAGAGGCCGTCGACCCTGATCCCGACCAGGTCATTCGATACAGCATCGTCCAGTCGC 360
3722R-2.IR_full 361 CGTTCGAGCGACCCAAGTTCTTTATCAATCCCAGCACGGGCGTAATCTTCACCACCCACA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3722R-2.in silico 361 CGTTCGAGCGACCCAAGTTCTTTATCAATCCCAGCACGGGCGTAATCTTCACCACCCACA 420
3722R-2.IR_full 421 CATTCGATCGTGACGAGCCGATCCACGAGAAGTTCGTCTTCGTCACCGTCCAGGCCACGG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3722R-2.in silico 421 CATTCGATCGTGACGAGCCGATCCACGAGAAGTTCGTCTTCGTCACCGTCCAGGCCACGG 480
3722R-2.IR_full 481 ATAATGGTCTGCCCCCACTTG 501
|||||||||||||||||| ||
3722R-2.in silico 481 ATAATGGTCTGCCCCCAC-TG 501
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
5 |
NM_057374.2 |
CG3722-RA (shg), mRNA |
| 0 |
0 |
8 |
3 |
19 |
NM_132412.1 |
CG9817-RA (CG9817), mRNA |
| 0 |
0 |
5 |
2 |
5 |
NM_078768.2 |
CG9554-RB, transcript variant B (eya), mRNA |
| 0 |
0 |
5 |
2 |
5 |
NM_164693.1 |
CG9554-RA, transcript variant A (eya), mRNA |
| 0 |
0 |
4 |
2 |
9 |
NM_167595.3 |
CG12348-RE, transcript variant E (Sh), mRNA |
| 0 |
0 |
4 |
2 |
9 |
NM_167594.2 |
CG12348-RC, transcript variant C (Sh), mRNA |
| 0 |
0 |
3 |
3 |
5 |
NM_133033.1 |
CG12672-RA (CG12672), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_139560.2 |
CG14971-RA (CG14971), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_168088.1 |
CG32249-RA (CG32249), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_140447.1 |
CG9598-RA (CG9598), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_206004.1 |
CG10719-RC, transcript variant C (brat), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_134302.1 |
CG10719-RB, transcript variant B (brat), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_057597.2 |
CG10719-RA, transcript variant A (brat), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_167194.1 |
CG3001-RB, transcript variant B (Hex-A), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_080109.2 |
CG3001-RA, transcript variant A (Hex-A), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_134690.2 |
CG2807-RA (CG2807), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_164898.1 |
CG4916-RB, transcript variant B (me31B), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_001014645.1 |
CG31156-RB, transcript variant B (CG31156), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_078809.2 |
CG4916-RA, transcript variant A (me31B), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_140091.2 |
CG6757-RA (SH3PX1), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_142813.3 |
CG31156-RA, transcript variant A (CG31156), mRNA |
| 0 |
0 |
1 |
2 |
10 |
NM_132578.1 |
CG11146-RA (CG11146), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_136941.2 |
CG8828-RA (CG8828), mRNA |
| 0 |
0 |
0 |
13 |
18 |
NM_058149.3 |
CG3352-RA (ft), mRNA |
| 0 |
0 |
0 |
10 |
17 |
NM_206620.4 |
CG32782-RD, transcript variant D (tlk), mRNA |
| 0 |
0 |
0 |
10 |
17 |
NM_130717.3 |
CG32782-RC, transcript variant C (tlk), mRNA |
| 0 |
0 |
0 |
9 |
22 |
NM_164981.1 |
CG18789-RA (CG18789), mRNA |
| 0 |
0 |
0 |
9 |
16 |
NM_144407.2 |
CG18787-RA (CG18787), mRNA |
| 0 |
0 |
0 |
9 |
11 |
NM_132206.1 |
CG2253-RA (Upf2), mRNA |
| 0 |
0 |
0 |
8 |
104 |
NM_167335.1 |
CG4013-RC, transcript variant C (Smr), mRNA |
|
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