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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
2286R-3 |
| Symbol |
ND75 |
Full Name |
NADH:ubiquinone reductase 75kD subunit precursor |
| CG No |
CG2286 |
Old CG No |
CG2286 |
| Synonyms |
CG2286, ND75 |
| Accession No (Link to NCBI) |
NM_078528.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tan SWS, Yip GW, Suda T, Baeg GH.
Small Maf functions in the maintenance of germline stem cells in the Drosophila testis.
Redox Biol (2018) 15 125-134
[
PubMed ID = 29245136
]
[
RRC reference
]
Tan SWS, Lee QY, Wong BSE, Cai Y, Baeg GH.
Redox Homeostasis Plays Important Roles in the Maintenance of the Drosophila Testis Germline Stem Cells.
Stem Cell Reports (2017) 9(1) 342-354
[
PubMed ID = 28669604
]
[
RRC reference
]
Hochmuth CE, Biteau B, Bohmann D, Jasper H.
Redox regulation by Keap1 and Nrf2 controls intestinal stem cell proliferation in Drosophila.
Cell Stem Cell (2011) 8(2) 188-99
[
PubMed ID = 21295275
]
[
RRC reference
]
Lee KS, Wu Z, Song Y, Mitra SS, Feroze AH, Cheshier SH, Lu B.
Roles of PINK1, mTORC2, and mitochondria in preserving brain tumor-forming stem cells in a noncanonical Notch signaling pathway.
Genes Dev (2013) 27(24) 2642-7
[
PubMed ID = 24352421
]
[
RRC reference
]
Owusu-Ansah E, Banerjee U.
Reactive oxygen species prime Drosophila haematopoietic progenitors for differentiation.
Nature (2009) 461(7263) 537-41
[
PubMed ID = 19727075
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGGACCGCTAGTCAAGACCATC |
| Primer Seq. 3' |
aatctagaggtaccGCCTTAATGGCCTTGGCCACG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GGACCGCTAG TCAAGACCAT CATGACACGC TGCATCCACT GCACCCGCTG TGTGCGTTTC
0061 GCCTCCGAGA TTGCCGGCGT TGATGACCTG GGCACCACTG GCCGTGGCAA CGACATGCAG
0121 ATCGGCACCT ACGTGGAGAA GCTCTTCCTT ACCGAACTGT CCGGCAACGT GATCGACCTG
0181 TGCCCCGTGG GAGCGCTGAC CAACAAGCCC TACAGTTTTG TGGCCCGTCC CTGGGAGATC
0241 CGTAAGGTGA GCAGCATCGA TGTGCTGGAC GCCGTCGGCA GCAACATTGT CGTCAGCACC
0301 CGCACCAATG AGGtGCTGCG CATCCTGCCC CGTGAGAACG AGGACGTCAA CGAGGAATGG
0361 CTTGCTGACA AATCGCGCTT CGCCTGCGAC GGTCTGAAGC GCCAgCNCCT GGTGGCTCCC
0421 ATGGTGCGCA TgNCCAACGG CGAACTACAG GCCGTAGAGT GGGAGGGTGC CCTCATCGCC
0481 GNGgcCAAGG CCATTAANGC |
| in silico PCR Fragment |
0001 GGACCGCTAG TCAAGACCAT CATGACACGC TGCATCCACT GCACCCGCTG TGTGCGTTTC
0061 GCCTCCGAGA TTGCCGGCGT TGATGACCTG GGCACCACTG GCCGTGGCAA CGACATGCAG
0121 ATCGGCACCT ACGTGGAGAA GCTCTTCCTT ACCGAACTGT CCGGCAACGT GATCGACCTG
0181 TGCCCCGTGG GAGCGCTGAC CAACAAGCCC TACAGTTTTG TGGCCCGTCC CTGGGAGATC
0241 CGTAAGGTGA GCAGCATCGA TGTGCTGGAC GCCGTCGGCA GCAACATTGT CGTCAGCACC
0301 CGCACCAATG AGGTGCTGCG CATCCTGCCC CGTGAGAACG AGGACGTCAA CGAGGAATGG
0361 CTTGCTGACA AATCGCGCTT CGCCTGCGAC GGTCTGAAGC GCCAGCGCCT GGTGGCTCCC
0421 ATGGTGCGCA TGCCCAACGG CGAACTACAG GCCGTAGAGT GGGAGGGTGC CCTCATCGCC
0481 GTGGCCAAGG CCATTAAGGC |
| Assemble Data |
2286R-3.IR_full 1 GGACCGCTAGTCAAGACCATCATGACACGCTGCATCCACTGCACCCGCTGTGTGCGTTTC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2286R-3.in silico 1 GGACCGCTAGTCAAGACCATCATGACACGCTGCATCCACTGCACCCGCTGTGTGCGTTTC 60
2286R-3.IR_full 61 GCCTCCGAGATTGCCGGCGTTGATGACCTGGGCACCACTGGCCGTGGCAACGACATGCAG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2286R-3.in silico 61 GCCTCCGAGATTGCCGGCGTTGATGACCTGGGCACCACTGGCCGTGGCAACGACATGCAG 120
2286R-3.IR_full 121 ATCGGCACCTACGTGGAGAAGCTCTTCCTTACCGAACTGTCCGGCAACGTGATCGACCTG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2286R-3.in silico 121 ATCGGCACCTACGTGGAGAAGCTCTTCCTTACCGAACTGTCCGGCAACGTGATCGACCTG 180
2286R-3.IR_full 181 TGCCCCGTGGGAGCGCTGACCAACAAGCCCTACAGTTTTGTGGCCCGTCCCTGGGAGATC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2286R-3.in silico 181 TGCCCCGTGGGAGCGCTGACCAACAAGCCCTACAGTTTTGTGGCCCGTCCCTGGGAGATC 240
2286R-3.IR_full 241 CGTAAGGTGAGCAGCATCGATGTGCTGGACGCCGTCGGCAGCAACATTGTCGTCAGCACC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2286R-3.in silico 241 CGTAAGGTGAGCAGCATCGATGTGCTGGACGCCGTCGGCAGCAACATTGTCGTCAGCACC 300
2286R-3.IR_full 301 CGCACCAATGAGGTGCTGCGCATCCTGCCCCGTGAGAACGAGGACGTCAACGAGGAATGG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2286R-3.in silico 301 CGCACCAATGAGGTGCTGCGCATCCTGCCCCGTGAGAACGAGGACGTCAACGAGGAATGG 360
2286R-3.IR_full 361 CTTGCTGACAAATCGCGCTTCGCCTGCGACGGTCTGAAGCGCCAGCNCCTGGTGGCTCCC 420
|||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
2286R-3.in silico 361 CTTGCTGACAAATCGCGCTTCGCCTGCGACGGTCTGAAGCGCCAGCGCCTGGTGGCTCCC 420
2286R-3.IR_full 421 ATGGTGCGCATGNCCAACGGCGAACTACAGGCCGTAGAGTGGGAGGGTGCCCTCATCGCC 480
|||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
2286R-3.in silico 421 ATGGTGCGCATGCCCAACGGCGAACTACAGGCCGTAGAGTGGGAGGGTGCCCTCATCGCC 480
2286R-3.IR_full 481 GNGGCCAAGGCCATTAANGC 500
| ||||||||||||||| ||
2286R-3.in silico 481 GTGGCCAAGGCCATTAAGGC 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_167152.1 |
CG2286-RB, transcript variant B (ND75), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_078528.1 |
CG2286-RA, transcript variant A (ND75), mRNA |
| 0 |
0 |
3 |
2 |
10 |
NM_143367.2 |
CG10011-RA (CG10011), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_131946.3 |
CG12184-RA (CG12184), mRNA |
| 0 |
0 |
2 |
5 |
3 |
NM_170168.1 |
CG6863-RB, transcript variant B (tok), mRNA |
| 0 |
0 |
2 |
5 |
3 |
NM_057531.3 |
CG6863-RA, transcript variant A (tok), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_079311.2 |
CG5638-RA (Rh7), mRNA |
| 0 |
0 |
2 |
2 |
12 |
NM_143018.2 |
CG5794-RD, transcript variant D (CG5794), mRNA |
| 0 |
0 |
2 |
2 |
12 |
NM_170158.1 |
CG5794-RB, transcript variant B (CG5794), mRNA |
| 0 |
0 |
2 |
2 |
10 |
NM_206564.1 |
CG5794-RE, transcript variant E (CG5794), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_001014494.1 |
CG33509-RA (CG33509), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_079188.1 |
CG1132-RA (fd64A), mRNA |
| 0 |
0 |
1 |
6 |
3 |
NM_134655.1 |
CG11620-RA (CG11620), mRNA |
| 0 |
0 |
1 |
5 |
9 |
NM_176232.1 |
CG15097-RB, transcript variant B (CG15097), mRNA |
| 0 |
0 |
1 |
5 |
9 |
NM_137533.3 |
CG15097-RA, transcript variant A (CG15097), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_169966.1 |
CG31198-RA (CG31198), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_136907.1 |
CG13169-RA (CG13169), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_132437.1 |
CG15207-RA (CG15207), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_137822.2 |
CG13506-RA (CG13506), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_078726.2 |
CG4385-RB, transcript variant B (S), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_135895.2 |
CG15269-RA (CG15269), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_167618.1 |
CG32551-RA (CG32551), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_141084.2 |
CG7162-RA (MED1), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_141681.1 |
CG9475-RA, transcript variant A (Rpt3R), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_169308.2 |
CG9475-RB, transcript variant B (Rpt3R), mRNA |
| 0 |
0 |
0 |
7 |
8 |
NM_168547.1 |
CG10738-RA, transcript variant A (CG10738), mRNA |
| 0 |
0 |
0 |
7 |
8 |
NM_140396.1 |
CG10738-RB, transcript variant B (CG10738), mRNA |
| 0 |
0 |
0 |
7 |
4 |
NM_132053.1 |
CG6041-RA (CG6041), mRNA |
| 0 |
0 |
0 |
7 |
3 |
NM_079917.2 |
CG18296-RA (axo), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_143026.1 |
CG6879-RA (CG6879), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| ++ |
|
| DB |
 |
| ++ |
|
| DC |
 |
| ++ |
|
| DM |
 |
| |
|
| VM |
 |
| |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| ++ |
|
| DB |
|
| ++ |
|
| DC |
|
| ++ |
|
| DM |
|
| + |
|
| VM |
|
| |
|
| VB |
|
| |
|
|
|
|
|
|
