NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 13348R-2

Symbol Aats-phe Full Name Phenylalanyl-tRNA synthetase

CG No CG13348 Old CG No CG13348

Synonyms unnamed, PheRS, FRS, Pts, CG13348, Aats-phe

Accession No (Link to NCBI) NM_057935.4

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Lindström R, Lindholm P, Palgi M, Saarma M, Heino TI.
In vivo screening reveals interactions between Drosophila Manf and genes involved in the mitochondria and the ubiquinone synthesis pathway.
BMC Genet (2017) 18(1) 52 [ PubMed ID = 28578657 ] [ RRC reference ]

Arsham AM, Neufeld TP.
A genetic screen in Drosophila reveals novel cytoprotective functions of the autophagy-lysosome pathway.
PLoS One (2009) 4(6) e6068 [ PubMed ID = 19562034 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGGAGCACGGCACTGGTTGAAG

Primer Seq. 3'

aatctagaggtaccACCGGATAGTGGGTGCTATCG

Predicted Fragment Size

IR fragment full Seq

0001 GGAGCACGGC ACTGGTTGAA GTCCACCCGC TGCCTGGCCA GCANGTGCgG CGCCCGCGAA 
0061 GTCGCCGTCA TCGCCACCGC AGCTGGAGGT CAGTGGAAGC ACGTACGCCA CCGACGGATG 
0121 GACGAACGTA ACGCCCAAGA TACTCTCCTA CGTGGGGGCC AACAANGCAC CTGCAGACGG 
0181 ACCACCCGCT GTCCATCATC CGCCAGAGGA TCGTCAACTA CTTTTATGGA GCGTATCNCA 
0241 ACCAAAGGGG CAATCCACTA TTCTCCGTCT ATGATCAAAT GAATCCGGTG GTTACTGTGC 
0301 AGCAGAACTT TGACAATCTG CTAATTCCCG CCGATCATGT GAGTCGACAG AAATCCGATT 
0361 GCTATTACAT CAATCAGCAG CATCTGCTGC GCGCCCACAC CACCGCCCAT CAGGTGGAAT 
0421 TGATCTCGGG CGGGCTAGAC AACTTTCTGG TCGTGGGCGA GGTGTATCGA CGGGACGAAA 
0481 TCGATAGCAC CCACTATCCG GT

in silico PCR Fragment

0001 GGAGCACGGC ACTGGTTGAA GTCCACCCGC TGCCTGGCCA GCA-GTGCGG CGCCCGCGAA 
0061 GTCGCCGTCA TCGCCACCGC AGCTGGAGGT CAGTGGAAGC ACGTACGCCA CCGACGGATG 
0121 GACGAACGTA ACGCCCAAGA TACTCTCCTA CGTGGGGGCC AACAA-GCAC CTGCAGACGG 
0181 ACCACCCGCT TTCCATCATC CGCCAGAGGA TCGTAAACTA CTTTTATGGA GCGTATCGCA 
0241 ACCAAAGGGG CAATCCACTA TTCTCCGTCT ATGATCAAAT GAATCCGGTG GTTACTGTGC 
0301 AGCAGAACTT TGACAATCTG CTAATTCCCG CCGATCATGT GAGTCGACAG AAATCCGATT 
0361 GCTATTACAT CAATCAGCAG CATCTGCTGC GCGCCCACAC CACCGCCCAT CAGGTGGAAT 
0421 TGATCTCGGG CGGGCTGGAC AACTTTCTGG TCGTGGGCGA GGTGTATCGA CGGGACGAAA 
0481 TCGATAGCAC CCACTATCCG GT

Assemble Data


13348R-2.IR_full       1   GGAGCACGGCACTGGTTGAAGTCCACCCGCTGCCTGGCCAGCANGTGCGGCGCCCGCGAA 60
                           ||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||
13348R-2.in silico     1   GGAGCACGGCACTGGTTGAAGTCCACCCGCTGCCTGGCCAGCA-GTGCGGCGCCCGCGAA 60


13348R-2.IR_full       61  GTCGCCGTCATCGCCACCGCAGCTGGAGGTCAGTGGAAGCACGTACGCCACCGACGGATG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13348R-2.in silico     61  GTCGCCGTCATCGCCACCGCAGCTGGAGGTCAGTGGAAGCACGTACGCCACCGACGGATG 120


13348R-2.IR_full       121 GACGAACGTAACGCCCAAGATACTCTCCTACGTGGGGGCCAACAANGCACCTGCAGACGG 180
                           ||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||
13348R-2.in silico     121 GACGAACGTAACGCCCAAGATACTCTCCTACGTGGGGGCCAACAA-GCACCTGCAGACGG 180


13348R-2.IR_full       181 ACCACCCGCTGTCCATCATCCGCCAGAGGATCGTCAACTACTTTTATGGAGCGTATCNCA 240
                           |||||||||| ||||||||||||||||||||||| |||||||||||||||||||||| ||
13348R-2.in silico     181 ACCACCCGCTTTCCATCATCCGCCAGAGGATCGTAAACTACTTTTATGGAGCGTATCGCA 240


13348R-2.IR_full       241 ACCAAAGGGGCAATCCACTATTCTCCGTCTATGATCAAATGAATCCGGTGGTTACTGTGC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13348R-2.in silico     241 ACCAAAGGGGCAATCCACTATTCTCCGTCTATGATCAAATGAATCCGGTGGTTACTGTGC 300


13348R-2.IR_full       301 AGCAGAACTTTGACAATCTGCTAATTCCCGCCGATCATGTGAGTCGACAGAAATCCGATT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13348R-2.in silico     301 AGCAGAACTTTGACAATCTGCTAATTCCCGCCGATCATGTGAGTCGACAGAAATCCGATT 360


13348R-2.IR_full       361 GCTATTACATCAATCAGCAGCATCTGCTGCGCGCCCACACCACCGCCCATCAGGTGGAAT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13348R-2.in silico     361 GCTATTACATCAATCAGCAGCATCTGCTGCGCGCCCACACCACCGCCCATCAGGTGGAAT 420


13348R-2.IR_full       421 TGATCTCGGGCGGGCTAGACAACTTTCTGGTCGTGGGCGAGGTGTATCGACGGGACGAAA 480
                           |||||||||||||||| |||||||||||||||||||||||||||||||||||||||||||
13348R-2.in silico     421 TGATCTCGGGCGGGCTGGACAACTTTCTGGTCGTGGGCGAGGTGTATCGACGGGACGAAA 480


13348R-2.IR_full       481 TCGATAGCACCCACTATCCGGT 502
                           ||||||||||||||||||||||
13348R-2.in silico     481 TCGATAGCACCCACTATCCGGT 502

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_057935.4	CG13348-RA (Aats-phe), mRNA
0	0	3	2	7	NM_057976.2	CG1897-RA (Dr), mRNA
0	0	3	2	4	NM_001014545.1	CG33519-RB (Unc-89), mRNA
0	0	3	2	3	NM_142112.2	CG8279-RA (Pde6), mRNA
0	0	2	3	2	NM_130730.1	CG2941-RA (CG2941), mRNA
0	0	2	2	6	NM_167309.1	CG32663-RA (CG32663), mRNA
0	0	2	2	3	NM_132635.1	CG4187-RA (CG4187), mRNA
0	0	1	6	19	NM_139681.1	CG13705-RA (CG13705), mRNA
0	0	1	6	4	NM_132273.1	CG2004-RA (CG2004), mRNA
0	0	1	4	8	NM_137194.1	CG8179-RA (CG8179), mRNA
0	0	1	4	5	NM_166623.1	CG4051-RA (egl), mRNA
0	0	1	4	2	NM_168938.1	CG7383-RB, transcript variant B (eg), mRNA
0	0	1	3	3	NM_169990.1	CG6343-RB, transcript variant B (ND42), mRNA
0	0	1	2	6	NM_078717.2	CG3696-RA, transcript variant A (kis), mRNA
0	0	1	2	5	NM_142853.1	CG6688-RA (CG6688), mRNA
0	0	1	2	4	NM_078772.2	CG13772-RA (neuroligin), mRNA
0	0	1	2	4	NM_140349.2	CG10948-RB, transcript variant B (CG10948), mRNA
0	0	1	2	4	NM_168524.1	CG10948-RC, transcript variant C (CG10948), mRNA
0	0	1	2	4	NM_168525.1	CG10948-RA, transcript variant A (CG10948), mRNA
0	0	1	2	3	NM_164376.1	CG3696-RB, transcript variant B (kis), mRNA
0	0	1	2	2	NM_141383.1	CG15189-RA (Osi19), mRNA
0	0	1	2	2	NM_137252.2	CG8445-RA, transcript variant A (CG8445), mRNA
0	0	1	2	2	NM_176194.1	CG8445-RB, transcript variant B (CG8445), mRNA
0	0	0	7	5	NM_142314.2	CG10324-RA (CG10324), mRNA
0	0	0	5	8	NM_167329.2	CG17762-RC, transcript variant C (tomosyn), mRNA
0	0	0	5	7	NM_079659.2	CG3937-RA, transcript variant A (cher), mRNA
0	0	0	5	7	NM_206502.1	CG3937-RD, transcript variant D (cher), mRNA
0	0	0	5	2	NM_057798.2	CG1650-RA (unpg), mRNA
0	0	0	5	2	NM_143237.2	CG5480-RA (CG5480), mRNA
0	0	0	4	10	NM_132778.1	CG9095-RA (CG9095), mRNA

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