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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 11154R-3 
 Symbol ATPsyn-beta  Full Name ATP synthase-beta 
 CG No CG11154  Old CG No CG11154 
 Synonyms ATP, ATPsyn-b, CG11154, ATPsyn b, beta-ATPase, ATPB, ATPsyn-beta 
 Accession No (Link to NCBI) NM_166808.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Kim K, Kim SH, Kim J, Kim H, Yim J.
Glutathione s-transferase omega 1 activity is sufficient to suppress neurodegeneration in a Drosophila model of Parkinson disease.
J Biol Chem (2012) 287(9) 6628-41 [ PubMed ID = 22219196 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGCGGACAGAAGGTTCTCGATA 
 Primer Seq. 3' 
aatctagaggtaccCAGTCCAGTTAGAGCAACACG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GCGGACAGAA GGTTCTCGAT ACTGGGTATC CTATCCGAAT TCCAGTTGGT GCCGAAACAC 
0061 TAGGACGCAT CATTAATGTT ATTGGTGAAC CCATTGATGA GCGTGGACCA ATTGATACAG 
0121 ACAAGACCGC TGCCATTCAT GCTGAGGCTC CTGAATTCGT TCAGATGTCT GTTGAGCAAG 
0181 AGATTCTTGT CACTGGAATC AAAGTCGTCG ATCTCCTGGC TCCATACGCC AAAGGTGGTA 
0241 AAATTGGGTT GTTTGGAGGT GCCGGTGTGG GCAAAACTGT GTTGATCATG GAGCTCATTA 
0301 ACAACGTGGC TAAAGCTCAT GGTGGATACT CGGTTTTCGC GGGAGTTGGT GAACGCACTC 
0361 GTGAGGGAAA CGATTTATAC AATGAGATGA TCGAGGGTGG TGTTATTTCA CTAAAGGACA 
0421 AGACCTCAAA AGGTGGCTCT CGTATATGGG CAAATGAACG AGCCGCCAGG TGCTCGTGCC 
0481 CGTGTTGCTC TAACTGGACT G 
 in silico PCR Fragment 
0001 GCGGACAGAA GGTTCTCGAT ACTGGGTATC CTATCCGAAT TCCAGTTGGT GCCGAAACAC 
0061 TAGGACGCAT CATTAATGTT ATTGGTGAAC CCATTGATGA GCGTGGACCA ATTGATACAG 
0121 ACAAGACCGC TGCCATTCAT GCTGAGGCTC CTGAATTCGT TCAGATGTCT GTTGAGCAAG 
0181 AGATTCTTGT CACTGGAATC AAAGTCGTCG ATCTCCTGGC TCCATACGCC AAAGGTGGTA 
0241 AAATTGGGTT GTTTGGAGGT GCCGGTGTGG GCAAAACTGT GTTGATCATG GAGCTCATTA 
0301 ACAACGTGGC TAAAGCTCAT GGTGGATACT CGGTTTTCGC GGGAGTTGGT GAACGCACTC 
0361 GTGAGGGAAA CGATTTATAC AATGAGATGA TCGAGGGTGG TGTTATTTCA CTAAAGGACA 
0421 AGACCTC-AA AGGTGGCTCT CGTATATGGG CAAATGAACG AGCCGCCAGG TGCTCGTGCC 
0481 CGTGTTGCTC TAACTGGACT G 
 Assemble Data 

11154R-3.IR_full       1   GCGGACAGAAGGTTCTCGATACTGGGTATCCTATCCGAATTCCAGTTGGTGCCGAAACAC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11154R-3.in silico     1   GCGGACAGAAGGTTCTCGATACTGGGTATCCTATCCGAATTCCAGTTGGTGCCGAAACAC 60


11154R-3.IR_full       61  TAGGACGCATCATTAATGTTATTGGTGAACCCATTGATGAGCGTGGACCAATTGATACAG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11154R-3.in silico     61  TAGGACGCATCATTAATGTTATTGGTGAACCCATTGATGAGCGTGGACCAATTGATACAG 120


11154R-3.IR_full       121 ACAAGACCGCTGCCATTCATGCTGAGGCTCCTGAATTCGTTCAGATGTCTGTTGAGCAAG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11154R-3.in silico     121 ACAAGACCGCTGCCATTCATGCTGAGGCTCCTGAATTCGTTCAGATGTCTGTTGAGCAAG 180


11154R-3.IR_full       181 AGATTCTTGTCACTGGAATCAAAGTCGTCGATCTCCTGGCTCCATACGCCAAAGGTGGTA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11154R-3.in silico     181 AGATTCTTGTCACTGGAATCAAAGTCGTCGATCTCCTGGCTCCATACGCCAAAGGTGGTA 240


11154R-3.IR_full       241 AAATTGGGTTGTTTGGAGGTGCCGGTGTGGGCAAAACTGTGTTGATCATGGAGCTCATTA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11154R-3.in silico     241 AAATTGGGTTGTTTGGAGGTGCCGGTGTGGGCAAAACTGTGTTGATCATGGAGCTCATTA 300


11154R-3.IR_full       301 ACAACGTGGCTAAAGCTCATGGTGGATACTCGGTTTTCGCGGGAGTTGGTGAACGCACTC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11154R-3.in silico     301 ACAACGTGGCTAAAGCTCATGGTGGATACTCGGTTTTCGCGGGAGTTGGTGAACGCACTC 360


11154R-3.IR_full       361 GTGAGGGAAACGATTTATACAATGAGATGATCGAGGGTGGTGTTATTTCACTAAAGGACA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11154R-3.in silico     361 GTGAGGGAAACGATTTATACAATGAGATGATCGAGGGTGGTGTTATTTCACTAAAGGACA 420


11154R-3.IR_full       421 AGACCTCAAAAGGTGGCTCTCGTATATGGGCAAATGAACGAGCCGCCAGGTGCTCGTGCC 480
                           ||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||
11154R-3.in silico     421 AGACCTC-AAAGGTGGCTCTCGTATATGGGCAAATGAACGAGCCGCCAGGTGCTCGTGCC 480


11154R-3.IR_full       481 CGTGTTGCTCTAACTGGACTG 501
                           |||||||||||||||||||||
11154R-3.in silico     481 CGTGTTGCTCTAACTGGACTG 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_166808.1  CG11154-RA, transcript variant A (ATPsyn-beta), mRNA 
93.98   453  3  3  2  NM_166809.1  CG11154-RB, transcript variant B (ATPsyn-beta), mRNA 
0   0  4  2  4  NM_137496.1  CG5226-RA (CG5226), mRNA 
0   0  1  2  3  NM_142083.2  CG14355-RA (CG14355), mRNA 
0   0  0  9  53  NM_140579.1  CG5389-RA (CG5389), mRNA 
0   0  0  5  5  NM_057442.3  CG15288-RA, transcript variant A (wb), mRNA 
0   0  0  5  5  NM_165086.1  CG15288-RB, transcript variant B (wb), mRNA 
0   0  0  5  4  NM_078867.2  CG5526-RA (Dhc36C), mRNA 
0   0  0  4  4  NM_001043133.1  CG32045-RD, transcript variant D (fry), mRNA 
0   0  0  4  4  NM_168357.2  CG32045-RB, transcript variant B (fry), mRNA 
0   0  0  4  2  NM_001043292.1  CG34110-RC (CG34110), mRNA 
0   0  0  3  12  NM_175960.3  CG33196-RB (dp), mRNA 
0   0  0  3  6  NM_134768.3  CG17660-RA (CG17660), mRNA 
0   0  0  3  4  NM_142168.1  CG6752-RA (CG6752), mRNA 
0   0  0  3  3  NM_164390.1  CG3727-RC, transcript variant C (dock), mRNA 
0   0  0  3  3  NM_164389.1  CG3727-RB, transcript variant B (dock), mRNA 
0   0  0  3  3  NM_057628.3  CG3727-RA, transcript variant A (dock), mRNA 
0   0  0  3  3  NM_168078.1  CG15013-RB, transcript variant B (dyl), mRNA 
0   0  0  3  3  NM_139633.2  CG15013-RA, transcript variant A (dyl), mRNA 
0   0  0  3  3  NM_168079.1  CG15013-RC, transcript variant C (dyl), mRNA 
0   0  0  3  2  NM_142966.2  CG6182-RA (CG6182), mRNA 
0   0  0  2  8  NM_140463.3  CG9384-RA (CG9384), mRNA 
0   0  0  2  8  NM_170158.1  CG5794-RB, transcript variant B (CG5794), mRNA 
0   0  0  2  8  NM_143018.2  CG5794-RD, transcript variant D (CG5794), mRNA 
0   0  0  2  6  NM_143586.2  CG1736-RA (Prosalpha3T), mRNA 
0   0  0  2  6  NM_135124.1  CG14001-RA (bchs), mRNA 
0   0  0  2  6  NM_142046.1  CG9591-RA (omd), mRNA 
0   0  0  2  5  NM_205902.1  CG3304-RB, transcript variant B (CG3304), mRNA 
0   0  0  2  5  NM_080045.2  CG6093-RA (abo), mRNA 
0   0  0  2  5  NM_139405.1  CG12020-RA (CG12020), mRNA 

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