NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

9807R-2

Symbol

Rab9Db

Full Name

Rab GTPase 9Db

CG No

CG9807

Old CG No

CG9807

Synonyms

Rab9Db, CG9807

Accession No (Link to NCBI)

NM_132414.1

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGAGCCAATACGACAATCCGTT

Primer Seq. 3'

aatctagaggtaccACCAGCAGGACGGTTACCTTA

Predicted Fragment Size

IR fragment full Seq

0001 GAGCCAATAC GACAATCCGT TTAAGATTAT CATCCTGGGT GACAGCGGCG TAGGCAAAAC 
0061 CTGCCTGCTG ATGCGTTTCT CGGATAACCA ATTCACCGAA AGACACCGGG TCACGGTGGG 
0121 CATGGATAGA AGGGAGTGCA GCGTGGAGTT TGCCGATTGG AGGATGGGGA GGATGCTGCA 
0181 AGTCTGGGAC ACCTCCGACG ACGAGCGCTT CAAATTGCTG AAGGCGACCC AATGTCGCAG 
0241 TGCCCATGGC ATACTGCTCG TCTACGACAT AACGTCGTCC AAAAGTTTCC AAAACATCGA 
0301 TGGCTGGATG AAGGAGATAC GGCGCCTGTG TCCGGATAAG GTAACCGTCC TGCTGGT

in silico PCR Fragment

0001 GAGCCAATAC GACAATCCGT TTAAGATTAT CATCCTGGGT GACAGCGGCG TAGGCAAAAC 
0061 CTGCCTGCTG ATGCGTTTCT CGGATAACCA ATTCACCGAA AGACACCGGG TCACGGTGGG 
0121 CATGGATAGA AGGGAGTGCA GCGTGGAGTT TGCCGATTGG AGGATGGGGA GGATGCTGCA 
0181 AGTCTGGGAC ACCTCCGACG ACGAGCGCTT CAAATTGCTG AAGGCGACCC AATGTCGCAG 
0241 TGCCCATGGC ATACTGCTCG TCTACGACAT AACGTCGTCC AAAAGTTTCC AAAACATCGA 
0301 TGGCTGGATG AAGGAGATAC GGCGCCTGTG TCCGGATAAG GTAACCGTCC TGCTGGT

Assemble Data


9807R-2.IR_full       1   GAGCCAATACGACAATCCGTTTAAGATTATCATCCTGGGTGACAGCGGCGTAGGCAAAAC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9807R-2.in silico     1   GAGCCAATACGACAATCCGTTTAAGATTATCATCCTGGGTGACAGCGGCGTAGGCAAAAC 60


9807R-2.IR_full       61  CTGCCTGCTGATGCGTTTCTCGGATAACCAATTCACCGAAAGACACCGGGTCACGGTGGG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9807R-2.in silico     61  CTGCCTGCTGATGCGTTTCTCGGATAACCAATTCACCGAAAGACACCGGGTCACGGTGGG 120


9807R-2.IR_full       121 CATGGATAGAAGGGAGTGCAGCGTGGAGTTTGCCGATTGGAGGATGGGGAGGATGCTGCA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9807R-2.in silico     121 CATGGATAGAAGGGAGTGCAGCGTGGAGTTTGCCGATTGGAGGATGGGGAGGATGCTGCA 180


9807R-2.IR_full       181 AGTCTGGGACACCTCCGACGACGAGCGCTTCAAATTGCTGAAGGCGACCCAATGTCGCAG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9807R-2.in silico     181 AGTCTGGGACACCTCCGACGACGAGCGCTTCAAATTGCTGAAGGCGACCCAATGTCGCAG 240


9807R-2.IR_full       241 TGCCCATGGCATACTGCTCGTCTACGACATAACGTCGTCCAAAAGTTTCCAAAACATCGA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9807R-2.in silico     241 TGCCCATGGCATACTGCTCGTCTACGACATAACGTCGTCCAAAAGTTTCCAAAACATCGA 300


9807R-2.IR_full       301 TGGCTGGATGAAGGAGATACGGCGCCTGTGTCCGGATAAGGTAACCGTCCTGCTGGT 357
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9807R-2.in silico     301 TGGCTGGATGAAGGAGATACGGCGCCTGTGTCCGGATAAGGTAACCGTCCTGCTGGT 357

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	339	0	0	0	NM_132414.1	CG9807-RA (CG9807), mRNA
87.31	296	8	15	8	NM_167260.1	CG32671-RA (CG32671), mRNA
79.64	270	22	27	4	NM_167238.1	CG32673-RA (CG32673), mRNA
77.87	264	28	27	8	NM_167237.1	CG32678-RA (Rab9D), mRNA
25.66	87	40	55	37	NM_132399.1	CG2885-RA (RabX2), mRNA
7.66	26	78	45	50	NM_167261.1	CG32670-RA (CG32670), mRNA
0	0	3	2	3	NM_001042857.1	CG17515-RA, transcript variant A (Rab21), mRNA
0	0	3	2	3	NM_001042856.1	CG17515-RB, transcript variant B (Rab21), mRNA
0	0	2	4	2	NM_079568.2	CG9366-RA (RhoL), mRNA
0	0	1	3	5	NM_206232.1	CG7852-RC, transcript variant C (CG7852), mRNA
0	0	1	3	4	NM_206227.1	CG12038-RA, transcript variant A (CG12038), mRNA
0	0	1	3	4	NM_206226.1	CG12038-RB, transcript variant B (CG12038), mRNA
0	0	1	2	4	NM_136672.2	CG1688-RA (CG1688), mRNA
0	0	1	2	2	NM_136673.2	CG1698-RA (CG1698), mRNA
0	0	1	2	2	NM_134481.1	CG14220-RA (CG14220), mRNA
0	0	0	6	7	NM_057863.3	CG8418-RA (Ric), mRNA
0	0	0	6	4	NM_133138.1	CG7884-RA (CG7884), mRNA
0	0	0	5	6	NM_132177.2	CG12156-RA (Rab39), mRNA
0	0	0	5	5	NM_142942.3	CG31140-RA, transcript variant A (CG31140), mRNA
0	0	0	5	2	NM_078664.2	CG5870-RA (beta-Spec), mRNA
0	0	0	4	3	NM_167193.1	CG32702-RA (CG32702), mRNA
0	0	0	4	2	NM_165083.2	CG31769-RA (CG31769), mRNA
0	0	0	3	14	NM_078695.2	CG17060-RA (Rab10), mRNA
0	0	0	3	6	NM_140447.1	CG9598-RA (CG9598), mRNA
0	0	0	3	6	NM_132740.1	CG15890-RA (CG15890), mRNA
0	0	0	3	5	NM_143815.2	CG3953-RA (l(3)IX-14), mRNA
0	0	0	3	4	NM_166446.1	CG9696-RD, transcript variant D (dom), mRNA
0	0	0	3	4	NM_080094.2	CG9696-RA, transcript variant A (dom), mRNA
0	0	0	3	4	NM_058124.2	CG5772-RA (Sur), mRNA
0	0	0	3	4	NM_176244.1	CG9696-RE, transcript variant E (dom), mRNA

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