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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 9777R-3 
 Symbol CG9777  Full Name CG9777 
 CG No CG9777  Old CG No CG9777 
 Synonyms CG9777 
 Accession No (Link to NCBI) NM_132910.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees pupal lethal 
 Map Viewer
 Reference
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 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgAAAGAAGCCAATGCCGCCAAA 
 Primer Seq. 3' 
aatctagaggtaccTATCGTTGCTATCGTTGCCTG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 AAAGAAGCCA ATGCCGCCAA ATTCTTTGAA TAATGGCGGC AAAACAAACA ATTCCGTGGG 
0061 TAATACCAAC AATCCGGCCG GAAATCTAAG TCCTGTTGCC GAAGAATGTG TGCCGCATCC 
0121 AAAGAACANA AGAGCGTACG CTCGTTGAGG ATTTGGCCAA AATAAATAAA CTGTTCGAGT 
0181 ATCTGGAGGC TTCAGCTACG GACGATATGG ATTTAGATAA TTCAACTGTC GAGGATGTGG 
0241 GAAAGGAAAG TTTATCGAAT ACGGTGCAGG CATGGAAAGA AGTTCCCAAT ATAAAAAATA 
0301 AATATTTTAT CGGAGAACAG GTGCCTGTGA TGGATACTTC CGACTTTATG CTTATTTCTC 
0361 GCAACAAGGC AAAGAAGACT TTGCGTGCCA GGCAAATGCG TCTTGAGCAG GAAAGAAAGG 
0421 TGGCTCTGGA AGCCGCGAAA TTGACCGAAC ANAAAAGGCA TGGGGGATCC TTCTTCGGTT 
0481 CCAGGCAACG ATAGCAACGA TA 
 in silico PCR Fragment 
0001 AAAGAAGCCA ATGCCGCCAA ATTCTTTGAA TAATGGCGGC AAAACAAACA ATTCCGTGGG 
0061 TAATACCAAC AATCCGGCCG GAAATCTAAG TCCTGTTGCC GAAGAATGTG TGCCGCATCC 
0121 AAAGAACA-A AGAGCGTACG CTCGTTGAGG ATTTGGCCAA AATAAATAAA CTGTTCGAGT 
0181 ATCTGGAGGC TTCAGCTACG GACGATATGG ATTTAGATAA TTCAACTGTC GAGGATGTGG 
0241 GAAAGGAAAG TTTATCGAAT ACGGTGCAGG CATGGAAAGA AGTTCCCAAT ATAAAAAATA 
0301 AATATTTTAT CGGAGAACAG GTGCCTGTGA TGGATACTTC CGACTTTATG CTTATTTCTC 
0361 GCAACAAGGC AAAGAAGACT TTGCGTGCCA GGCAAATGCG TCTTGAGCAG GAAAGAAAGG 
0421 TGGCTCTGGA AGCCGCGAAA TTGACCGAAC A-AAAAGGCA TGGGGGATCC TTCTTCGGTT 
0481 CCAGGCAACG ATAGCAACGA TA 
 Assemble Data 

9777R-3.IR_full       1   AAAGAAGCCAATGCCGCCAAATTCTTTGAATAATGGCGGCAAAACAAACAATTCCGTGGG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9777R-3.in silico     1   AAAGAAGCCAATGCCGCCAAATTCTTTGAATAATGGCGGCAAAACAAACAATTCCGTGGG 60


9777R-3.IR_full       61  TAATACCAACAATCCGGCCGGAAATCTAAGTCCTGTTGCCGAAGAATGTGTGCCGCATCC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9777R-3.in silico     61  TAATACCAACAATCCGGCCGGAAATCTAAGTCCTGTTGCCGAAGAATGTGTGCCGCATCC 120


9777R-3.IR_full       121 AAAGAACANAAGAGCGTACGCTCGTTGAGGATTTGGCCAAAATAAATAAACTGTTCGAGT 180
                          |||||||| |||||||||||||||||||||||||||||||||||||||||||||||||||
9777R-3.in silico     121 AAAGAACA-AAGAGCGTACGCTCGTTGAGGATTTGGCCAAAATAAATAAACTGTTCGAGT 180


9777R-3.IR_full       181 ATCTGGAGGCTTCAGCTACGGACGATATGGATTTAGATAATTCAACTGTCGAGGATGTGG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9777R-3.in silico     181 ATCTGGAGGCTTCAGCTACGGACGATATGGATTTAGATAATTCAACTGTCGAGGATGTGG 240


9777R-3.IR_full       241 GAAAGGAAAGTTTATCGAATACGGTGCAGGCATGGAAAGAAGTTCCCAATATAAAAAATA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9777R-3.in silico     241 GAAAGGAAAGTTTATCGAATACGGTGCAGGCATGGAAAGAAGTTCCCAATATAAAAAATA 300


9777R-3.IR_full       301 AATATTTTATCGGAGAACAGGTGCCTGTGATGGATACTTCCGACTTTATGCTTATTTCTC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9777R-3.in silico     301 AATATTTTATCGGAGAACAGGTGCCTGTGATGGATACTTCCGACTTTATGCTTATTTCTC 360


9777R-3.IR_full       361 GCAACAAGGCAAAGAAGACTTTGCGTGCCAGGCAAATGCGTCTTGAGCAGGAAAGAAAGG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
9777R-3.in silico     361 GCAACAAGGCAAAGAAGACTTTGCGTGCCAGGCAAATGCGTCTTGAGCAGGAAAGAAAGG 420


9777R-3.IR_full       421 TGGCTCTGGAAGCCGCGAAATTGACCGAACANAAAAGGCATGGGGGATCCTTCTTCGGTT 480
                          ||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||
9777R-3.in silico     421 TGGCTCTGGAAGCCGCGAAATTGACCGAACA-AAAAGGCATGGGGGATCCTTCTTCGGTT 480


9777R-3.IR_full       481 CCAGGCAACGATAGCAACGATA 502
                          ||||||||||||||||||||||
9777R-3.in silico     481 CCAGGCAACGATAGCAACGATA 502



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  2  2  NM_132910.1  CG9777-RA (CG9777), mRNA 
0   0  2  3  4  NM_139845.2  CG8605-RA (CG8605), mRNA 
0   0  2  3  3  NM_164349.1  CG2671-RA, transcript variant A (l(2)gl), mRNA 
0   0  2  3  3  NM_078715.2  CG2671-RC, transcript variant C (l(2)gl), mRNA 
0   0  2  3  3  NM_164352.1  CG2671-RF, transcript variant F (l(2)gl), mRNA 
0   0  2  3  3  NM_164348.1  CG2671-RB, transcript variant B (l(2)gl), mRNA 
0   0  2  3  3  NM_164351.1  CG2671-RE, transcript variant E (l(2)gl), mRNA 
0   0  2  3  3  NM_164350.1  CG2671-RD, transcript variant D (l(2)gl), mRNA 
0   0  2  2  5  NM_132385.2  CG2967-RA (CG2967), mRNA 
0   0  2  2  4  NM_142644.1  CG5466-RA (CG5466), mRNA 
0   0  1  7  3  NM_142751.1  CG5778-RA (CG5778), mRNA 
0   0  1  4  2  NM_133044.2  CG12611-RA (Andorra), mRNA 
0   0  1  3  10  NM_001038766.1  CG32529-RD, transcript variant D (CG32529), mRNA 
0   0  1  3  3  NM_078686.2  CG12230-RA, transcript variant A (car), mRNA 
0   0  1  3  3  NM_167664.1  CG12230-RB, transcript variant B (car), mRNA 
0   0  1  3  3  NM_167596.3  CG12348-RA, transcript variant A (Sh), mRNA 
0   0  1  3  3  NM_167592.2  CG12348-RD, transcript variant D (Sh), mRNA 
0   0  1  3  2  NM_078929.2  CG11205-RA, transcript variant A (phr), mRNA 
0   0  1  3  2  NM_165564.1  CG11205-RB, transcript variant B (phr), mRNA 
0   0  1  2  6  NM_079803.2  CG6127-RA (Ser), mRNA 
0   0  1  2  6  NM_165049.1  CG31814-RA (CG31814), mRNA 
0   0  1  2  5  NM_167562.1  CG10598-RB, transcript variant B (CG10598), mRNA 
0   0  1  2  5  NM_080192.2  CG10449-RA (Catsup), mRNA 
0   0  1  2  5  NM_132978.2  CG10598-RA, transcript variant A (CG10598), mRNA 
0   0  1  2  3  NM_080064.2  CG32505-RA, transcript variant A (Pp4-19C), mRNA 
0   0  1  2  3  NM_167703.3  CG32505-RE, transcript variant E (Pp4-19C), mRNA 
0   0  1  2  2  NM_137866.1  CG13524-RA (Obp58c), mRNA 
0   0  1  1  3  NM_132961.2  CG4955-RA (CG4955), mRNA 
0   0  0  4  5  NM_165549.2  CG1399-RA, transcript variant A (CG1399), mRNA 
0   0  0  4  4  NM_078867.2  CG5526-RA (Dhc36C), mRNA 

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