NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 8719R-2

Symbol Odc2 Full Name Ornithine decarboxylase 2

CG No CG8719 Old CG No CG8719

Synonyms dodc2, CG8719, Odc2

Accession No (Link to NCBI) NM_057705.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
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Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCCATCGAGAAGGCGGAAAACA

Primer Seq. 3'

aatctagaggtaccGTAGGCTCCCATATTGCGAAA

Predicted Fragment Size

IR fragment full Seq

0001 CCNTCGAGAA GGGCGGAAAA CATCTTTAAG GTCGGCGAGA TGATAGGTCA CAAGATGGAA 
0061 CTATTGGATG TTGGCGGTGG CTTTCCTGGC ATTGATGATG AGATGTTCGA GGAAATAGCG 
0121 CAGTCGGTAA ACACATCAGT GGAGCTGCGT TTTCCCGATA AGCGTATCCG AATCATTTCC 
0181 GAGCCAGGAC GCTTCTTTGT GGAGTCCGCA TACACATTGA TCTGCAAGGT ACATGCCAAG 
0241 CGGGAAGTCC GCAGCAAGGA TGGCAAGCTG GACACCATGA TGTACTACCT GAATGATGGA 
0301 ATCTTCGGAG CGTTCGCAGG AATGTTTTAC TACCCTGAGG AGGTGGCTCC AGAGCTTTAC 
0361 TTGGACGAAG CCGAGAGCTT GCCAAAACTC AAGTCTGTGA TTTGGGGACC CAGCTGCGAT 
0421 GCTTTGGATA AGATATCGGA CTTGCTATTG CCCAACTTGA ATCCGGGCGA TCTATTAGGC 
0481 TTTCGCAATA TGGGAGcCTN C

in silico PCR Fragment

0001 CCATCGAGAA -GGCGGAAAA CATCTTTAAG GTCGGCGAGA TGATAGGTCA CAAGATGGAA 
0061 CTATTGGATG TTGGCGGTGG CTTTCCTGGC ATTGATGATG AGATGTTCGA GGAAATAGCG 
0121 CAGTCGGTAA ACACATCAGT GGAGCTGCGT TTTCCCGATA AGCGTATCCG AATCATTTCC 
0181 GAGCCAGGAC GCTTCTTTGT GGAGGCCGCA TACACATTGA TCTGCAAGGT ACATGCCAAG 
0241 CGGGAAGTCC GCAGCAAGGA TGGCAAGCTG GACACCATGA TGTACTACCT GAATGATGGA 
0301 ATCTTCGGAG CGTTCGCAGG AATGTTTTAC TACCCTGAAG AGGTGGCTCC CGAGCTCTAC 
0361 TTGGACGAAG CCGAGAGCTT GCCAAAACTC AAGTCTGTGA TTTGGGGACC CAGCTGCGAT 
0421 GCTATGGATA AGATATCGGA CTTGCTATTG CCCAACTTGA ATCCGGGCGA TCTATTAGGC 
0481 TTTCGCAATA TGGGAGCCTA C

Assemble Data


8719R-2.IR_full       1   CCNTCGAGAAGGGCGGAAAACATCTTTAAGGTCGGCGAGATGATAGGTCACAAGATGGAA 60
                          || ||||||| |||||||||||||||||||||||||||||||||||||||||||||||||
8719R-2.in silico     1   CCATCGAGAA-GGCGGAAAACATCTTTAAGGTCGGCGAGATGATAGGTCACAAGATGGAA 60


8719R-2.IR_full       61  CTATTGGATGTTGGCGGTGGCTTTCCTGGCATTGATGATGAGATGTTCGAGGAAATAGCG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8719R-2.in silico     61  CTATTGGATGTTGGCGGTGGCTTTCCTGGCATTGATGATGAGATGTTCGAGGAAATAGCG 120


8719R-2.IR_full       121 CAGTCGGTAAACACATCAGTGGAGCTGCGTTTTCCCGATAAGCGTATCCGAATCATTTCC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8719R-2.in silico     121 CAGTCGGTAAACACATCAGTGGAGCTGCGTTTTCCCGATAAGCGTATCCGAATCATTTCC 180


8719R-2.IR_full       181 GAGCCAGGACGCTTCTTTGTGGAGTCCGCATACACATTGATCTGCAAGGTACATGCCAAG 240
                          |||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||
8719R-2.in silico     181 GAGCCAGGACGCTTCTTTGTGGAGGCCGCATACACATTGATCTGCAAGGTACATGCCAAG 240


8719R-2.IR_full       241 CGGGAAGTCCGCAGCAAGGATGGCAAGCTGGACACCATGATGTACTACCTGAATGATGGA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8719R-2.in silico     241 CGGGAAGTCCGCAGCAAGGATGGCAAGCTGGACACCATGATGTACTACCTGAATGATGGA 300


8719R-2.IR_full       301 ATCTTCGGAGCGTTCGCAGGAATGTTTTACTACCCTGAGGAGGTGGCTCCAGAGCTTTAC 360
                          |||||||||||||||||||||||||||||||||||||| ||||||||||| ||||| |||
8719R-2.in silico     301 ATCTTCGGAGCGTTCGCAGGAATGTTTTACTACCCTGAAGAGGTGGCTCCCGAGCTCTAC 360


8719R-2.IR_full       361 TTGGACGAAGCCGAGAGCTTGCCAAAACTCAAGTCTGTGATTTGGGGACCCAGCTGCGAT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8719R-2.in silico     361 TTGGACGAAGCCGAGAGCTTGCCAAAACTCAAGTCTGTGATTTGGGGACCCAGCTGCGAT 420


8719R-2.IR_full       421 GCTTTGGATAAGATATCGGACTTGCTATTGCCCAACTTGAATCCGGGCGATCTATTAGGC 480
                          ||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8719R-2.in silico     421 GCTATGGATAAGATATCGGACTTGCTATTGCCCAACTTGAATCCGGGCGATCTATTAGGC 480


8719R-2.IR_full       481 TTTCGCAATATGGGAGCCTNC 501
                          ||||||||||||||||||| |
8719R-2.in silico     481 TTTCGCAATATGGGAGCCTAC 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_057705.2	CG8719-RA (Odc2), mRNA
0.2	1	9	12	41	NM_057704.3	CG8721-RA (Odc1), mRNA
0	0	3	3	2	NM_144487.1	CG5518-RA (sda), mRNA
0	0	1	3	3	NM_130545.2	CG11411-RA (fs(1)N), mRNA
0	0	1	3	2	NM_166238.2	CG4853-RA, transcript variant A (CG4853), mRNA
0	0	1	3	2	NM_137378.2	CG4853-RB, transcript variant B (CG4853), mRNA
0	0	1	2	5	NM_132440.2	CG2145-RA (CG2145), mRNA
0	0	1	2	2	NM_137288.2	CG4439-RA (CG4439), mRNA
0	0	0	5	5	NM_079211.2	CG10583-RA (Sse), mRNA
0	0	0	5	5	NM_140773.1	CG5137-RA (Cyp312a1), mRNA
0	0	0	5	2	NM_141378.2	CG1155-RA (Osi14), mRNA
0	0	0	4	10	NM_164454.1	CG31671-RA (tho2), mRNA
0	0	0	4	6	NM_139490.1	CG12093-RA (CG12093), mRNA
0	0	0	4	5	NM_141419.3	CG2358-RA (Spase18-21), mRNA
0	0	0	4	4	NM_137078.2	CG8067-RA (CG8067), mRNA
0	0	0	4	3	NM_080046.2	CG10986-RB (g), mRNA
0	0	0	4	2	NM_132960.2	CG4949-RA, transcript variant A (CG4949), mRNA
0	0	0	4	2	NM_167554.1	CG4949-RB, transcript variant B (CG4949), mRNA
0	0	0	4	2	NM_134943.1	CG3213-RA (CG3213), mRNA
0	0	0	4	2	NM_137861.2	CG12190-RA (CG12190), mRNA
0	0	0	4	2	NM_140422.2	CG17359-RA (CG17359), mRNA
0	0	0	4	2	NM_164836.1	CG12437-RA, transcript variant A (raw), mRNA
0	0	0	3	8	NM_175960.3	CG33196-RB (dp), mRNA
0	0	0	3	8	NM_078592.2	CG11172-RA (NFAT), mRNA
0	0	0	3	7	NM_137759.3	CG30263-RA (CG30263), mRNA
0	0	0	3	6	NM_135341.1	CG8552-RA (CG8552), mRNA
0	0	0	3	5	NM_205928.1	CG8086-RD, transcript variant D (CG8086), mRNA
0	0	0	3	5	NM_143571.2	CG15544-RA (CG15544), mRNA
0	0	0	3	4	NM_166610.1	CG5393-RD, transcript variant D (apt), mRNA
0	0	0	3	4	NM_057832.3	CG5393-RA, transcript variant A (apt), mRNA

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