NIG-Fly - Fly Stocks of National Institute of Genetics -

Notice:
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.

NIG-RNAi Strains Detail

Request : Click the "Order" button to request for this stock

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

8534R-4

Symbol

CG8534

Full Name

CG8534

CG No

CG8534

Old CG No

CG8534

Synonyms

CG8534

Accession No (Link to NCBI)

NM_141698.1

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCATCGGAAGTCCATGGCCATC

Primer Seq. 3'

aatctagaggtaccTGCTGACGGAGGACAGGTAGT

Predicted Fragment Size

IR fragment full Seq

0001 CATCGGAAGT CCATGGCCAT CGCTGACCAT CGTTTCGCTC TATCTGCTGT TTGTCTTGAA 
0061 GTTAGGCAGG AAGTTCATGG AGAACAGAAA GCCCTACGAT CTCCGCAGAG TCATCAGGGC 
0121 ATACAACATT ATGCAGATTG TCTACAACGG CGTTATTCTG ATAGCGGGTC TTCACTTTTT 
0181 GTTCGTCCTG AAGGCCTACG ACCTGCGATG CATCACCAAA CTGCCGCTGG ATCACGAGCT 
0241 GAAGAGCCGA GAAAGATGGT TGACCTACTC GTACTTCTTC AACAAGTTCA TGGATCTGCT 
0301 GGAGACTGTG TTCTTTGTGC TGCGCAAGAA GGATCGCCAG ATATCCTTCC TGCACGTCTT 
0361 CCACCACCTG GTGATGTCCT TTGGCGGATA CCTGCACATT ACCTTCAACG GCTATGGAGG 
0421 CACTCTGTTC CCATTATGCC TGCTCAATGT GGCGGTCCAT GTGATCATGT ACGCCTACTA 
0481 CTACCTGTCC TCCGTCAGCA

in silico PCR Fragment

0001 CATCGGAAGT CCATGGCCAT CGCTGACCAT CGTTTCGCTC TATCTGCTGT TTGTCTTGAA 
0061 GTTAGGCAGG AAGTTCATGG AGAACAGAAA GCCCTACGAT CTCCGCAGAG TCATCAGGGC 
0121 ATACAACATT ATGCAGATTG TCTACAACGG CGTTATTCTG ATAGCGGGTC TTCACTTTTT 
0181 GTTCGTCCTG AAGGCCTACG ACCTGCGATG CATCACCAAA CTGCCGCTGG ATCACGAGCT 
0241 GAAGAGCCGA GAAAGATGGT TGACCTACTC GTACTTCTTC AACAAGTTCA TGGATCTGCT 
0301 GGAGACTGTG TTCTTTGTGC TGCGCAAGAA GGATCGCCAG ATATCCTTCC TGCACGTCTT 
0361 CCACCACCTG GTGATGTCCT TTGGCGGATA CCTGCACATT ACCTTCAACG GCTATGGAGG 
0421 CACTCTGTTC CCATTATGCC TGCTCAATGT GGCGGTCCAT GTGATCATGT ACGCCTACTA 
0481 CTACCTGTCC TCCGTCAGCA

Assemble Data


8534R-4.IR_full       1   CATCGGAAGTCCATGGCCATCGCTGACCATCGTTTCGCTCTATCTGCTGTTTGTCTTGAA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8534R-4.in silico     1   CATCGGAAGTCCATGGCCATCGCTGACCATCGTTTCGCTCTATCTGCTGTTTGTCTTGAA 60


8534R-4.IR_full       61  GTTAGGCAGGAAGTTCATGGAGAACAGAAAGCCCTACGATCTCCGCAGAGTCATCAGGGC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8534R-4.in silico     61  GTTAGGCAGGAAGTTCATGGAGAACAGAAAGCCCTACGATCTCCGCAGAGTCATCAGGGC 120


8534R-4.IR_full       121 ATACAACATTATGCAGATTGTCTACAACGGCGTTATTCTGATAGCGGGTCTTCACTTTTT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8534R-4.in silico     121 ATACAACATTATGCAGATTGTCTACAACGGCGTTATTCTGATAGCGGGTCTTCACTTTTT 180


8534R-4.IR_full       181 GTTCGTCCTGAAGGCCTACGACCTGCGATGCATCACCAAACTGCCGCTGGATCACGAGCT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8534R-4.in silico     181 GTTCGTCCTGAAGGCCTACGACCTGCGATGCATCACCAAACTGCCGCTGGATCACGAGCT 240


8534R-4.IR_full       241 GAAGAGCCGAGAAAGATGGTTGACCTACTCGTACTTCTTCAACAAGTTCATGGATCTGCT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8534R-4.in silico     241 GAAGAGCCGAGAAAGATGGTTGACCTACTCGTACTTCTTCAACAAGTTCATGGATCTGCT 300


8534R-4.IR_full       301 GGAGACTGTGTTCTTTGTGCTGCGCAAGAAGGATCGCCAGATATCCTTCCTGCACGTCTT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8534R-4.in silico     301 GGAGACTGTGTTCTTTGTGCTGCGCAAGAAGGATCGCCAGATATCCTTCCTGCACGTCTT 360


8534R-4.IR_full       361 CCACCACCTGGTGATGTCCTTTGGCGGATACCTGCACATTACCTTCAACGGCTATGGAGG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8534R-4.in silico     361 CCACCACCTGGTGATGTCCTTTGGCGGATACCTGCACATTACCTTCAACGGCTATGGAGG 420


8534R-4.IR_full       421 CACTCTGTTCCCATTATGCCTGCTCAATGTGGCGGTCCATGTGATCATGTACGCCTACTA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8534R-4.in silico     421 CACTCTGTTCCCATTATGCCTGCTCAATGTGGCGGTCCATGTGATCATGTACGCCTACTA 480


8534R-4.IR_full       481 CTACCTGTCCTCCGTCAGCA 500
                          ||||||||||||||||||||
8534R-4.in silico     481 CTACCTGTCCTCCGTCAGCA 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_141698.1	CG8534-RA (CG8534), mRNA
2.28	11	34	31	19	NM_141701.2	CG9459-RA (CG9459), mRNA
1.65	8	12	26	32	NM_141699.1	CG16905-RA (CG16905), mRNA
1.24	6	2	5	23	NM_170023.1	CG6921-RC, transcript variant C (CG6921), mRNA
1.24	6	2	5	23	NM_170022.1	CG6921-RA, transcript variant A (CG6921), mRNA
1.24	6	2	5	23	NM_142805.3	CG6921-RB, transcript variant B (CG6921), mRNA
1.03	5	35	35	28	NM_169312.1	CG9458-RA (CG9458), mRNA
1.03	5	4	14	22	NM_142803.1	CG5326-RA, transcript variant A (CG5326), mRNA
0.82	4	2	4	7	NM_176539.1	CG33110-RA (CG33110), mRNA
0.62	3	6	14	15	NM_141700.1	CG16904-RA (CG16904), mRNA
0.41	2	6	11	20	NM_142806.1	CG5278-RA (CG5278), mRNA
0	0	6	7	12	NM_137521.1	CG18609-RA (CG18609), mRNA
0	0	3	7	9	NM_132075.3	CG3781-RA (CG3781), mRNA
0	0	3	3	5	NM_142389.2	CG7655-RA (CG7655), mRNA
0	0	3	2	2	NM_139990.2	CG5971-RA (CG5971), mRNA
0	0	1	10	20	NM_141497.1	CG2781-RA (CG2781), mRNA
0	0	1	6	11	NM_165718.1	CG30008-RA (CG30008), mRNA
0	0	1	5	4	NM_134500.2	CG14224-RA (CG14224), mRNA
0	0	1	3	3	NM_139801.2	CG10103-RA (CG10103), mRNA
0	0	1	3	2	NM_166498.1	CG13499-RC, transcript variant C (CG13499), mRNA
0	0	1	3	2	NM_137784.1	CG13499-RB, transcript variant B (CG13499), mRNA
0	0	1	3	2	NM_166499.1	CG13499-RD, transcript variant D (CG13499), mRNA
0	0	1	3	2	NM_166497.1	CG13499-RA, transcript variant A (CG13499), mRNA
0	0	1	2	5	NM_139441.2	CG8960-RA, transcript variant A (CG8960), mRNA
0	0	1	2	2	NM_001032029.1	CG33934-RA, transcript variant A (CG33934), mRNA
0	0	1	2	2	NM_001032028.1	CG33934-RB, transcript variant B (CG33934), mRNA
0	0	1	2	2	NM_001032031.1	CG33933-RA, transcript variant A (Indy-2), mRNA
0	0	1	2	2	NM_143616.3	CG1635-RA (CG1635), mRNA
0	0	1	2	2	NM_142367.1	CG5866-RA (CG5866), mRNA
0	0	0	8	16	NM_170095.1	CG31141-RA (CG31141), mRNA

Back