NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 8137R-1

Symbol Spn2 Full Name Serine protease inhibitor 2

CG No CG8137 Old CG No CG8137

Synonyms CG8137, sp2, Sp2, Spn2

Accession No (Link to NCBI) NM_080218.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCTGAAGCGGAGGCAATCGACA

Primer Seq. 3'

aatctagaggtaccTATGCCCATCGCCACAAGAAC

Predicted Fragment Size

IR fragment full Seq

0001 CTGAAGCGGA NGCAATCGAC ATTGTCgNTC CCAATAAGGC TTCTTCAATT GTCAATAATT 
0061 GGGTAGATAA TCAGACGCGT GGTAAAATCA AAGACTTAGT TTCATCCAAT GACATGTCGA 
0121 AAATGGAATT GATCGTCTTA AATGCCATAT ACTTTAAAGG CCAGTGGGAG TACAAATTTA 
0181 ATCCCAAGTT AACGAAGAAG AGGAATTTCC GTGTTTCGGA TCAAAAGAGT GTTCCTGTCG 
0241 AGATGCATGT CACTATTTCA ATCATTTAGG GCCGCACATG ATAGTGAACT GGGTGCCAAA 
0301 ATTATCGAGC TGCCCTACAG AAATTCAAGC CTGTCCATGC TAATTTTTCT ACCGGACCAG 
0361 GTCGATGGAC TATCAGAGCT GGAGAAAAAG ATTGTAGGAT TCAAACCGAA ACTTTCCAAA 
0421 ATGGATGTAA CTTTAAGGCT TCCCAAGTTT AAAATCGAAT TCTTCGCTCA ACTAAACAAA 
0481 GTTCTTGTGG CGATGGGCAT A

in silico PCR Fragment

0001 CTGAAGCGGA GGCAATCGAC ATTGTCGATC CCAATAAGGC TTCTTCAATT GTCAATAATT 
0061 GGGTAGATAA TCAGACGCGT GGTAAAATCA AAGACTTAGT TTCATCCAAT GACATGTCGA 
0121 AAATGGAATT GATCGTCTTA AATGCCATAT ACTTTAAAGG CCAGTGGGAG TACAAATTTA 
0181 ATCCCAAGTT AACGAAGAAG AGGAATTTCC GTGTTTCGGA TCAAAAGAGT GTTCCTGTCG 
0241 AGATG-ATGT CACTATTTCA ATCATTTAGG GCCGCACATG ATAGTGAACT GGGTGCCAAA 
0301 ATTATCGAGC TGCCCTACAG AAATTCAAGC CTGTCCATGC TAATTTTTCT ACCGGACCAG 
0361 GTCGATGGAC TATCAGAGCT GGAGAAAAAG ATTGTAGGAT TCAAACCGAA ACTTTCCAAA 
0421 ATGGATGTAA CTTTAAGGCT TCCCAAGTTT AAAATCGAAT TCTTCGCTCA ACTAAACAAA 
0481 GTTCTTGTGG CGATGGGCAT A

Assemble Data


8137R-1.IR_full       1   CTGAAGCGGANGCAATCGACATTGTCGNTCCCAATAAGGCTTCTTCAATTGTCAATAATT 60
                          |||||||||| |||||||||||||||| ||||||||||||||||||||||||||||||||
8137R-1.in silico     1   CTGAAGCGGAGGCAATCGACATTGTCGATCCCAATAAGGCTTCTTCAATTGTCAATAATT 60


8137R-1.IR_full       61  GGGTAGATAATCAGACGCGTGGTAAAATCAAAGACTTAGTTTCATCCAATGACATGTCGA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8137R-1.in silico     61  GGGTAGATAATCAGACGCGTGGTAAAATCAAAGACTTAGTTTCATCCAATGACATGTCGA 120


8137R-1.IR_full       121 AAATGGAATTGATCGTCTTAAATGCCATATACTTTAAAGGCCAGTGGGAGTACAAATTTA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8137R-1.in silico     121 AAATGGAATTGATCGTCTTAAATGCCATATACTTTAAAGGCCAGTGGGAGTACAAATTTA 180


8137R-1.IR_full       181 ATCCCAAGTTAACGAAGAAGAGGAATTTCCGTGTTTCGGATCAAAAGAGTGTTCCTGTCG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8137R-1.in silico     181 ATCCCAAGTTAACGAAGAAGAGGAATTTCCGTGTTTCGGATCAAAAGAGTGTTCCTGTCG 240


8137R-1.IR_full       241 AGATGCATGTCACTATTTCAATCATTTAGGGCCGCACATGATAGTGAACTGGGTGCCAAA 300
                          ||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
8137R-1.in silico     241 AGATG-ATGTCACTATTTCAATCATTTAGGGCCGCACATGATAGTGAACTGGGTGCCAAA 300


8137R-1.IR_full       301 ATTATCGAGCTGCCCTACAGAAATTCAAGCCTGTCCATGCTAATTTTTCTACCGGACCAG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8137R-1.in silico     301 ATTATCGAGCTGCCCTACAGAAATTCAAGCCTGTCCATGCTAATTTTTCTACCGGACCAG 360


8137R-1.IR_full       361 GTCGATGGACTATCAGAGCTGGAGAAAAAGATTGTAGGATTCAAACCGAAACTTTCCAAA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8137R-1.in silico     361 GTCGATGGACTATCAGAGCTGGAGAAAAAGATTGTAGGATTCAAACCGAAACTTTCCAAA 420


8137R-1.IR_full       421 ATGGATGTAACTTTAAGGCTTCCCAAGTTTAAAATCGAATTCTTCGCTCAACTAAACAAA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
8137R-1.in silico     421 ATGGATGTAACTTTAAGGCTTCCCAAGTTTAAAATCGAATTCTTCGCTCAACTAAACAAA 480


8137R-1.IR_full       481 GTTCTTGTGGCGATGGGCATA 501
                          |||||||||||||||||||||
8137R-1.in silico     481 GTTCTTGTGGCGATGGGCATA 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_080218.2	CG8137-RA (Spn2), mRNA
3.52	17	72	103	62	NM_080217.2	CG9334-RA (Spn3), mRNA
0.41	2	9	14	28	NM_080219.3	CG9456-RA (Spn1), mRNA
0	0	3	4	3	NM_132125.1	CG4536-RA (CG4536), mRNA
0	0	1	5	4	NM_135716.2	CG17010-RA, transcript variant A (CG17010), mRNA
0	0	1	5	4	NM_165008.1	CG17010-RB, transcript variant B (CG17010), mRNA
0	0	1	4	11	NM_135284.1	CG6717-RA (Spn7), mRNA
0	0	1	4	2	NM_137050.1	CG6209-RA (CG6209), mRNA
0	0	1	2	5	NM_167487.1	CG32577-RA (disco-r), mRNA
0	0	1	2	2	NM_136826.1	CG7741-RA (CG7741), mRNA
0	0	0	7	4	NM_142710.2	CG16791-RA (CG16791), mRNA
0	0	0	5	7	NM_132793.2	CG9164-RA, transcript variant A (CG9164), mRNA
0	0	0	5	2	NM_170556.1	CG11525-RC, transcript variant C (CycG), mRNA
0	0	0	4	4	NM_137773.1	CG13493-RA (comr), mRNA
0	0	0	4	2	NM_167168.1	CG10701-RB, transcript variant B (Moe), mRNA
0	0	0	4	2	NM_167169.1	CG10701-RA, transcript variant A (Moe), mRNA
0	0	0	4	2	NM_135186.1	CG16947-RA (CG16947), mRNA
0	0	0	3	6	NM_001042880.1	CG31902-RB (CG31902), mRNA
0	0	0	3	5	NM_176539.1	CG33110-RA (CG33110), mRNA
0	0	0	3	5	NM_167886.1	CG1009-RD, transcript variant D (Psa), mRNA
0	0	0	3	4	NM_136054.2	CG10341-RA (CG10341), mRNA
0	0	0	3	4	NM_166100.1	CG30471-RA (CG30471), mRNA
0	0	0	3	3	NM_170430.2	CG31037-RA (ca), mRNA
0	0	0	3	3	NM_167965.1	CG32296-RA (Mrtf), mRNA
0	0	0	3	3	NM_136879.2	CG8364-RA, transcript variant A (Rep3), mRNA
0	0	0	3	2	NM_165178.1	CG17332-RD, transcript variant D (VhaSFD), mRNA
0	0	0	3	2	NM_078861.2	CG17332-RA, transcript variant A (VhaSFD), mRNA
0	0	0	3	2	NM_001032247.1	CG33155-RC, transcript variant C (CG33155), mRNA
0	0	0	3	2	NM_165177.1	CG17332-RB, transcript variant B (VhaSFD), mRNA
0	0	0	3	2	NM_166022.1	CG30481-RA, transcript variant A (mRpL53), mRNA

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