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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 7824R-2 
 Symbol CG7824  Full Name CG7824 
 CG No CG7824  Old CG No CG7824 
 Synonyms CG7824 
 Accession No (Link to NCBI) NM_143481.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGTAAAATCCGAGCCCCCTGAA 
 Primer Seq. 3' 
aatctagaggtaccACTACGTCTGAGCTATCGAAG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GTAAAATCCG AGCCCCCTGA AGAAAAGCCC CGCTTATCTC CACAGGACGC TAATCAAAAA 
0061 CGTGCAGTAA AGGAGGAGAA GGAAATGTCC CAGGCGCGCA CGCGCTCGAG CCAAGAAAAT 
0121 ACGGAATACA GAGGCAAGAG CAGCGAAATC GGTGGTTACC AGGGGTGCGG GTAAAAAAGC 
0181 AAGGAAGCGT AAGAAGCAAA ATAAAGCGGC ACTCAAGAAG GCTAGTGAAC TTGAACCCAA 
0241 AAAGGCAAAG GGCTCCAAGG AGGCTAGTGC CCACAAGAAG GCAAAGGGAC CCAAGAATAC 
0301 AAGTCCTCCT ATGGCTGCGA AGCCCAATAT GGCAAAGAGT GACAACCTGA CGATGGCCCT 
0361 GGTGGACGAG CTCCACGAAG ACGGTCGTCT GCTCAACGAA AGGTGGCAGG AGGTCGAGGC 
0421 CCAGCTGGCG TACATGGTGA CTGACAGAGT CAGTGGCCAT GCCGGGGGGT CCCGTCCCGT 
0481 CCTTCGATAG CTCAGACGTA GT 
 in silico PCR Fragment 
0001 GTAAAATCCG AGCCCCCTGA AGAAAAGCCC CGCTTATCTC CACAGGACGC TAATCAAAAA 
0061 CGTGCAGTAA AGGAGGAGAA GGAAATGT-C CAGGCGCGCA CGCGCTCGAG CCAAGAAAAT 
0121 ACGGAATACA GAGGCAAGAG CAGCGAAATC GGTGGTTACC AGGGGTGCGG GTAAAAAAGC 
0181 AAGGAAGCGT AAGAAGCAAA ATAAAGCGGC ACTCAAGAAG GCTAGTGAAC TTGAACCCAA 
0241 AAAGGCAAAG GGCTCCAAGG AGGCTAGTGC CCACAAGAAG GCAAAGGGAC CCAAGAATAC 
0301 AAGTCCTCCT ATGGCTGCGA AGCCCAATTT GGCAAAGAGT GACAACCTGA CGATGGCCCT 
0361 GGTGGACGAG CTCCACGAAG ACGGCCGTCT GCTTAACGAA AGGTGGCAGG AGGTCGAGGC 
0421 CCAGCTGGCG TACATGGTGA CTGACAGAGT CA-TGGCCAT GCCGGGGGGT CCCGTCCCGT 
0481 CCTTCGATAG CTCAGACGTA GT 
 Assemble Data 

7824R-2.IR_full       1   GTAAAATCCGAGCCCCCTGAAGAAAAGCCCCGCTTATCTCCACAGGACGCTAATCAAAAA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7824R-2.in silico     1   GTAAAATCCGAGCCCCCTGAAGAAAAGCCCCGCTTATCTCCACAGGACGCTAATCAAAAA 60


7824R-2.IR_full       61  CGTGCAGTAAAGGAGGAGAAGGAAATGTCCCAGGCGCGCACGCGCTCGAGCCAAGAAAAT 120
                          |||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||
7824R-2.in silico     61  CGTGCAGTAAAGGAGGAGAAGGAAATGT-CCAGGCGCGCACGCGCTCGAGCCAAGAAAAT 120


7824R-2.IR_full       121 ACGGAATACAGAGGCAAGAGCAGCGAAATCGGTGGTTACCAGGGGTGCGGGTAAAAAAGC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7824R-2.in silico     121 ACGGAATACAGAGGCAAGAGCAGCGAAATCGGTGGTTACCAGGGGTGCGGGTAAAAAAGC 180


7824R-2.IR_full       181 AAGGAAGCGTAAGAAGCAAAATAAAGCGGCACTCAAGAAGGCTAGTGAACTTGAACCCAA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7824R-2.in silico     181 AAGGAAGCGTAAGAAGCAAAATAAAGCGGCACTCAAGAAGGCTAGTGAACTTGAACCCAA 240


7824R-2.IR_full       241 AAAGGCAAAGGGCTCCAAGGAGGCTAGTGCCCACAAGAAGGCAAAGGGACCCAAGAATAC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7824R-2.in silico     241 AAAGGCAAAGGGCTCCAAGGAGGCTAGTGCCCACAAGAAGGCAAAGGGACCCAAGAATAC 300


7824R-2.IR_full       301 AAGTCCTCCTATGGCTGCGAAGCCCAATATGGCAAAGAGTGACAACCTGACGATGGCCCT 360
                          |||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||
7824R-2.in silico     301 AAGTCCTCCTATGGCTGCGAAGCCCAATTTGGCAAAGAGTGACAACCTGACGATGGCCCT 360


7824R-2.IR_full       361 GGTGGACGAGCTCCACGAAGACGGTCGTCTGCTCAACGAAAGGTGGCAGGAGGTCGAGGC 420
                          |||||||||||||||||||||||| |||||||| ||||||||||||||||||||||||||
7824R-2.in silico     361 GGTGGACGAGCTCCACGAAGACGGCCGTCTGCTTAACGAAAGGTGGCAGGAGGTCGAGGC 420


7824R-2.IR_full       421 CCAGCTGGCGTACATGGTGACTGACAGAGTCAGTGGCCATGCCGGGGGGTCCCGTCCCGT 480
                          |||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||
7824R-2.in silico     421 CCAGCTGGCGTACATGGTGACTGACAGAGTCA-TGGCCATGCCGGGGGGTCCCGTCCCGT 480


7824R-2.IR_full       481 CCTTCGATAGCTCAGACGTAGT 502
                          ||||||||||||||||||||||
7824R-2.in silico     481 CCTTCGATAGCTCAGACGTAGT 502



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  12  NM_143481.1  CG7824-RA, transcript variant A (CG7824), mRNA 
90.87   438  1  3  13  NM_170436.1  CG7824-RB, transcript variant B (CG7824), mRNA 
0   0  8  4  2  NM_079428.2  CG3889-RA (CSN1b), mRNA 
0   0  1  18  11  NM_170437.1  CG31032-RA (CG31032), mRNA 
0   0  1  4  2  NM_167497.2  CG3632-RA, transcript variant A (CG3632), mRNA 
0   0  1  4  2  NM_167499.2  CG3632-RC, transcript variant C (CG3632), mRNA 
0   0  1  4  2  NM_132884.2  CG3632-RD, transcript variant D (CG3632), mRNA 
0   0  1  2  4  NR_001754.1  CG3632-RD, transcript variant D (CG3632), mRNA, miscRNA 
0   0  1  2  2  NM_057224.3  CG4694-RA (her), mRNA 
0   0  0  4  11  NM_136653.3  CG1884-RA, transcript variant A (Not1), mRNA 
0   0  0  4  11  NM_165678.2  CG1884-RB, transcript variant B (Not1), mRNA 
0   0  0  4  6  NM_205982.1  CG6214-RM, transcript variant M (MRP), mRNA 
0   0  0  4  6  NM_205978.1  CG6214-RQ, transcript variant Q (MRP), mRNA 
0   0  0  4  6  NM_205968.1  CG6214-RL, transcript variant L (MRP), mRNA 
0   0  0  4  6  NM_205980.1  CG6214-RO, transcript variant O (MRP), mRNA 
0   0  0  4  6  NM_205971.1  CG6214-RI, transcript variant I (MRP), mRNA 
0   0  0  4  6  NM_135747.3  CG6214-RB, transcript variant B (MRP), mRNA 
0   0  0  4  6  NM_205976.1  CG6214-RD, transcript variant D (MRP), mRNA 
0   0  0  4  6  NM_205975.1  CG6214-RE, transcript variant E (MRP), mRNA 
0   0  0  4  6  NM_205969.1  CG6214-RK, transcript variant K (MRP), mRNA 
0   0  0  4  6  NM_205974.1  CG6214-RF, transcript variant F (MRP), mRNA 
0   0  0  4  6  NM_205981.1  CG6214-RN, transcript variant N (MRP), mRNA 
0   0  0  4  6  NM_205970.1  CG6214-RJ, transcript variant J (MRP), mRNA 
0   0  0  4  6  NM_205972.1  CG6214-RH, transcript variant H (MRP), mRNA 
0   0  0  4  6  NM_205979.1  CG6214-RP, transcript variant P (MRP), mRNA 
0   0  0  4  6  NM_205973.1  CG6214-RG, transcript variant G (MRP), mRNA 
0   0  0  4  6  NM_165020.2  CG6214-RA, transcript variant A (MRP), mRNA 
0   0  0  4  6  NM_205977.1  CG6214-RC, transcript variant C (MRP), mRNA 
0   0  0  4  3  NM_170360.1  CG4976-RA (Mes-4), mRNA 
0   0  0  4  2  NM_144103.1  CG13946-RA (CG13946), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Caspase-3 signal
N
+ 
DB
+ 
DC
+ 
DM
+ 
VM
+ 
VB
 
Dcompartment   Penetrance
     

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