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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 7815R-2 
 Symbol ran-like  Full Name ran-like 
 CG No CG7815  Old CG No CG7815 
 Synonyms CG7815, BcDNA:GH25818, anon-WO0140519.150, ran-like, Ran-like 
 Accession No (Link to NCBI) NM_079358.2 
 Inserted Chr. ll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		 
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATGCAACCTCAAGAGGAAGTG 
 Primer Seq. 3' 
aatctagaggtaccCGCAATAGATAGACGAATGGC 
 Predicted Fragment Size 
497 
 IR fragment full Seq 
0001 ATGCAACCTC AAGAGGAAGT GAAGGCCATT TTCAAGCTGA TTCTAATCGG AGACGGGGGA 
0061 ACTGGGAAAA CCACATTGGT CAAGCGACAT CTGACCGGCG AGTTCAAGAT GCAATACAAT 
0121 GCGACCTTGG GTGTGGAGGT CGAGCAATTA CTGTTTAACA CCAACAGAGG AGTTTTCCGC 
0181 ATCGATGTTT GGGACACTGC CGGTCAGGAG AGGTACGGTG GCCTGCGCGA TGGGTACTTC 
0241 GTCCAATCAC AATGTGCCAT AATAATGTTC GATGTGGCCT CGTCAAATAC ATATAATAAT 
0301 GTGAAAANGA TGGCACCGCG ACTTGGTGAG AGTATGCGGC AACATACCGA TTGTCATTTG 
0361 TGGCAACAAG GTGGATATCA TGCATAAAAA GACTTGGAAA AAGGGTGTTG ACTTTGATCG 
0421 CAAGACAAAC ATTTACCTCA TTGAAATGTC CGCAAAGTCG AACTATAACG TGGAGAAGCC 
0481 ATTCGTCTAT CTATTGCG 
 in silico PCR Fragment 
0001 ATGCAACCTC AAGAGGAAGT GAAGGCCATT TTCAAGCTGA TTCTAATCGG AGACGGGGGA 
0061 ACTGGGAAAA CCACATTGGT CAAGCGACAT CTGACCGGCG AGTTCAAGAT GCAATACAAT 
0121 GCGACCTTGG GTGTGGAGGT CGAGCAATTA CTGTTTAACA CCAACAGAGG AGTTTTCCGC 
0181 ATCGATGTTT GGGACACTGC CGGTCAGGAG AGGTACGGTG GCCTGCGCGA TGGGTACTTC 
0241 GTCCAATCAC AATGTGCCAT AATAATGTTC GATGTGGCCT CGTCAAATAC ATATAATAAT 
0301 GTGAAAA-GA TGGCACCGCG ACTTGGTGAG AGTATGCGGC AACATACCGA TTGTCATTTG 
0361 TGGCAACAAG GTGGATATCA TGCATAAAAA GACTTGGAAA AAGGGTGTTG ACTTTGATCG 
0421 CAAGACAAAC ATTTACCTCA TTGAAATGTC CGCAAAGTCG AACTATAACG TGGAGAAGCC 
0481 ATTCGTCTAT CTATTGCG 
 Assemble Data 

7815R-2.IR_full       1   ATGCAACCTCAAGAGGAAGTGAAGGCCATTTTCAAGCTGATTCTAATCGGAGACGGGGGA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7815R-2.in silico     1   ATGCAACCTCAAGAGGAAGTGAAGGCCATTTTCAAGCTGATTCTAATCGGAGACGGGGGA 60


7815R-2.IR_full       61  ACTGGGAAAACCACATTGGTCAAGCGACATCTGACCGGCGAGTTCAAGATGCAATACAAT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7815R-2.in silico     61  ACTGGGAAAACCACATTGGTCAAGCGACATCTGACCGGCGAGTTCAAGATGCAATACAAT 120


7815R-2.IR_full       121 GCGACCTTGGGTGTGGAGGTCGAGCAATTACTGTTTAACACCAACAGAGGAGTTTTCCGC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7815R-2.in silico     121 GCGACCTTGGGTGTGGAGGTCGAGCAATTACTGTTTAACACCAACAGAGGAGTTTTCCGC 180


7815R-2.IR_full       181 ATCGATGTTTGGGACACTGCCGGTCAGGAGAGGTACGGTGGCCTGCGCGATGGGTACTTC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7815R-2.in silico     181 ATCGATGTTTGGGACACTGCCGGTCAGGAGAGGTACGGTGGCCTGCGCGATGGGTACTTC 240


7815R-2.IR_full       241 GTCCAATCACAATGTGCCATAATAATGTTCGATGTGGCCTCGTCAAATACATATAATAAT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7815R-2.in silico     241 GTCCAATCACAATGTGCCATAATAATGTTCGATGTGGCCTCGTCAAATACATATAATAAT 300


7815R-2.IR_full       301 GTGAAAANGATGGCACCGCGACTTGGTGAGAGTATGCGGCAACATACCGATTGTCATTTG 360
                          ||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||
7815R-2.in silico     301 GTGAAAA-GATGGCACCGCGACTTGGTGAGAGTATGCGGCAACATACCGATTGTCATTTG 360


7815R-2.IR_full       361 TGGCAACAAGGTGGATATCATGCATAAAAAGACTTGGAAAAAGGGTGTTGACTTTGATCG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7815R-2.in silico     361 TGGCAACAAGGTGGATATCATGCATAAAAAGACTTGGAAAAAGGGTGTTGACTTTGATCG 420


7815R-2.IR_full       421 CAAGACAAACATTTACCTCATTGAAATGTCCGCAAAGTCGAACTATAACGTGGAGAAGCC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7815R-2.in silico     421 CAAGACAAACATTTACCTCATTGAAATGTCCGCAAAGTCGAACTATAACGTGGAGAAGCC 480


7815R-2.IR_full       481 ATTCGTCTATCTATTGCG 498
                          ||||||||||||||||||
7815R-2.in silico     481 ATTCGTCTATCTATTGCG 498



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   479  0  0  0  NM_079358.2  CG7815-RA (ran-like), mRNA 
0   0  7  3  2  NM_079246.3  CG7062-RA (Rab-RP3), mRNA 
0   0  5  4  5  NM_206155.1  CG4921-RC, transcript variant C (Rab4), mRNA 
0   0  5  4  5  NM_166240.1  CG4921-RA, transcript variant A (Rab4), mRNA 
0   0  5  4  5  NM_079053.2  CG4921-RB, transcript variant B (Rab4), mRNA 
0   0  3  4  4  NM_079448.2  CG8287-RA (Rab8), mRNA 
0   0  3  4  3  NM_139387.1  CG7980-RA (RabX5), mRNA 
0   0  3  3  2  NM_079568.2  CG9366-RA (RhoL), mRNA 
0   0  2  3  3  NM_079748.2  CG5915-RA (Rab7), mRNA 
0   0  1  5  4  NM_143288.2  CG31064-RE, transcript variant E (CG31064), mRNA 
0   0  1  5  4  NM_170325.1  CG31064-RA, transcript variant A (CG31064), mRNA 
0   0  1  2  2  NM_142497.2  CG18208-RA (CG18208), mRNA 
0   0  1  2  2  NM_164430.2  CG31666-RD, transcript variant D (CG31666), mRNA 
0   0  1  2  2  NM_134766.3  CG31666-RA, transcript variant A (CG31666), mRNA 
0   0  1  2  2  NM_164428.2  CG31666-RB, transcript variant B (CG31666), mRNA 
0   0  1  2  2  NM_164429.1  CG31666-RC, transcript variant C (CG31666), mRNA 
0   0  0  7  16  NM_144448.2  CG1915-RC, transcript variant C (sls), mRNA 
0   0  0  5  39  NM_143712.2  CG1404-RA, transcript variant A (ran), mRNA 
0   0  0  5  39  NM_167272.1  CG1404-RB, transcript variant B (ran), mRNA 
0   0  0  5  7  NM_001042856.1  CG17515-RB, transcript variant B (Rab21), mRNA 
0   0  0  5  7  NM_001042857.1  CG17515-RA, transcript variant A (Rab21), mRNA 
0   0  0  5  2  NM_138260.4  CG2199-RA, transcript variant A (CG2199), mRNA 
0   0  0  5  2  NM_167873.1  CG2199-RB, transcript variant B (CG2199), mRNA 
0   0  0  4  7  NM_079494.3  CG8779-RA, transcript variant A (nrm), mRNA 
0   0  0  4  7  NM_137622.2  CG11788-RA (CG11788), mRNA 
0   0  0  4  7  NM_001043156.1  CG8779-RB, transcript variant B (nrm), mRNA 
0   0  0  4  5  NM_139797.2  CG8519-RA (CG8519), mRNA 
0   0  0  4  5  NM_137759.3  CG30263-RA (CG30263), mRNA 
0   0  0  4  4  NM_169059.1  CG2902-RA (Nmdar1), mRNA 
0   0  0  3  12  NM_080370.3  CG8024-RB, transcript variant B (ltd), mRNA 

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