NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 7045R-2

Symbol CG7045 Full Name CG7045

CG No CG7045 Old CG No CG7045

Synonyms CG7045

Accession No (Link to NCBI) NM_142798.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCGCGCCCCAAAAAACCCATGA

Primer Seq. 3'

aatctagaggtaccGTGAAGGCACCTGGTCTTTGA

Predicted Fragment Size

IR fragment full Seq

0001 CGCGCCCCAA AAAACCCATG AGCGCCTTCA TGTTGTGGAT GAATTCCACC GGGCGCAAGC 
0061 ACATAAAAGC GGAGCATCCC GATTTTAGTG TCCAAGAAGT GTCTGTGAAG GGCGGAGAGA 
0121 TGTGGCGAGC CATGGCCGAT GAGGACAAGA TCGTGTGGCA GGAGTCGGCC ACCACGGCAA 
0181 TGGCCGAGTA CAAGGAGAAG TTGAAGCAGT GGAATTTCCC CAAGGAGCAC CGCTTTTCGG 
0241 ACACGCAATG TATTTGTTCC TCAAATACTA ACCAATGCCC CACCCTTTTT GTGTACGACA 
0301 CCATGGATGA CTCGATGACT CCGATCTGCA GGAAGTGCTT ATCAAAGACC AGGTGCCTTC 
0361 AC

in silico PCR Fragment

0001 CGCGCCCCAA AAAACCCATG AGCGCCTTCA TGTTGTGGAT GAATTCCACC GGGCGGAAGC 
0061 ACATAAAAGC GGAGCATCCC GATTTTAGTG TCCAAGAAGT GTCTGTGAAG GGCGGAGAGA 
0121 TGTGGCGAGC CATGGCCGAT GAGGACAAGA TCGTGTGGCA GGAGTCGGCC ACCACGGCAA 
0181 TGGCCGAGTA CAAGGAGAAG TTGAAGCAGT GGAATTTCCC CAAGGAGCAC CGCTTTTCGG 
0241 ACACGCAATG TATTTGTTCC TCAAATACTA ACCAATGCCC CACCCTTTTT GTGTACGACA 
0301 CCATGGATGA CTCGATGACT CCGATCTGCA GGAAGTGCTT ATCAAAGACC AGGTGCCTTC 
0361 AC

Assemble Data


7045R-2.IR_full       1   CGCGCCCCAAAAAACCCATGAGCGCCTTCATGTTGTGGATGAATTCCACCGGGCGCAAGC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||
7045R-2.in silico     1   CGCGCCCCAAAAAACCCATGAGCGCCTTCATGTTGTGGATGAATTCCACCGGGCGGAAGC 60


7045R-2.IR_full       61  ACATAAAAGCGGAGCATCCCGATTTTAGTGTCCAAGAAGTGTCTGTGAAGGGCGGAGAGA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7045R-2.in silico     61  ACATAAAAGCGGAGCATCCCGATTTTAGTGTCCAAGAAGTGTCTGTGAAGGGCGGAGAGA 120


7045R-2.IR_full       121 TGTGGCGAGCCATGGCCGATGAGGACAAGATCGTGTGGCAGGAGTCGGCCACCACGGCAA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7045R-2.in silico     121 TGTGGCGAGCCATGGCCGATGAGGACAAGATCGTGTGGCAGGAGTCGGCCACCACGGCAA 180


7045R-2.IR_full       181 TGGCCGAGTACAAGGAGAAGTTGAAGCAGTGGAATTTCCCCAAGGAGCACCGCTTTTCGG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7045R-2.in silico     181 TGGCCGAGTACAAGGAGAAGTTGAAGCAGTGGAATTTCCCCAAGGAGCACCGCTTTTCGG 240


7045R-2.IR_full       241 ACACGCAATGTATTTGTTCCTCAAATACTAACCAATGCCCCACCCTTTTTGTGTACGACA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7045R-2.in silico     241 ACACGCAATGTATTTGTTCCTCAAATACTAACCAATGCCCCACCCTTTTTGTGTACGACA 300


7045R-2.IR_full       301 CCATGGATGACTCGATGACTCCGATCTGCAGGAAGTGCTTATCAAAGACCAGGTGCCTTC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
7045R-2.in silico     301 CCATGGATGACTCGATGACTCCGATCTGCAGGAAGTGCTTATCAAAGACCAGGTGCCTTC 360


7045R-2.IR_full       361 AC 362
                          ||
7045R-2.in silico     361 AC 362

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	344	0	0	0	NM_142798.1	CG7045-RA (CG7045), mRNA
27.32	94	71	57	39	NM_142799.1	CG7046-RA (CG7046), mRNA
0.29	1	2	2	5	NM_135688.2	CG5202-RB, transcript variant B (escl), mRNA
0.29	1	2	2	5	NM_164979.1	CG5202-RA, transcript variant A (escl), mRNA
0	0	3	3	3	NM_140665.1	CG9674-RA, transcript variant A (CG9674), mRNA
0	0	3	3	3	NM_176339.1	CG9674-RD, transcript variant D (CG9674), mRNA
0	0	3	3	2	NM_169172.1	CG1070-RB, transcript variant B (Alh), mRNA
0	0	3	3	2	NM_169170.1	CG1070-RC, transcript variant C (Alh), mRNA
0	0	2	5	3	NM_057691.3	CG4654-RA, transcript variant A (Dp), mRNA
0	0	2	5	3	NM_165974.2	CG4654-RB, transcript variant B (Dp), mRNA
0	0	2	4	4	NM_141524.1	CG9626-RA (CG9626), mRNA
0	0	2	4	2	NM_144022.2	CG32428-RA (CG32428), mRNA
0	0	2	3	4	NM_169277.1	CG9354-RB, transcript variant B (RpL34b), mRNA
0	0	2	3	4	NM_141630.1	CG9354-RA, transcript variant A (RpL34b), mRNA
0	0	2	2	4	NM_138220.1	CG13889-RA (CG13889), mRNA
0	0	2	2	2	NM_142322.1	CG18622-RA (CG18622), mRNA
0	0	2	2	1	NM_142714.2	CG7922-RA (CG7922), mRNA
0	0	1	4	3	NM_001043246.1	CG4154-RD, transcript variant D (Gyc88E), mRNA
0	0	1	4	3	NM_142167.2	CG4154-RC, transcript variant C (Gyc88E), mRNA
0	0	1	4	3	NM_169616.2	CG4154-RA, transcript variant A (Gyc88E), mRNA
0	0	1	3	6	NM_057546.2	CG3090-RA, transcript variant A (Sox14), mRNA
0	0	1	3	6	NM_134290.1	CG3090-RB, transcript variant B (Sox14), mRNA
0	0	1	3	2	NM_078983.2	CG8439-RA, transcript variant A (Cct5), mRNA
0	0	1	2	7	NM_135051.3	CG31917-RA (CG31917), mRNA
0	0	1	2	6	NM_165844.1	CG10897-RB, transcript variant B (tou), mRNA
0	0	1	2	6	NM_165843.1	CG10897-RD, transcript variant D (tou), mRNA
0	0	1	2	6	NM_078977.2	CG10897-RA, transcript variant A (tou), mRNA
0	0	1	2	5	NM_165842.1	CG10897-RC, transcript variant C (tou), mRNA
0	0	1	2	4	NM_080349.2	CG7107-RA, transcript variant A (up), mRNA
0	0	1	2	4	NM_167376.1	CG7107-RD, transcript variant D (up), mRNA

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